Intercellular Adhesion Molecule-1 in Early Diagnosis of Neonatal Infection

2016 ◽  
Vol 11 (3) ◽  
pp. 88-94
Author(s):  
M.I. Abdel-Aal ◽  
M.M. Elmazahi ◽  
S. Abd Rabou ◽  
M.Z. El-Ghannam ◽  
F.M. Elries
Keyword(s):  
Early Diagnosis ◽  
Adhesion Molecule ◽  
Neonatal Infection ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1

Predictive Value of Soluble Immunological Mediators in Neonatal Infection

1994 ◽  
Vol 87 (2) ◽  
pp. 165-171 ◽  
Author(s):  
J. D. M. Edgar ◽  
D. C. Wilson ◽  
S. A. McMillan ◽  
A. D. Crockard ◽  
M. I. Halliday ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Interleukin 6 ◽  
Predictive Value ◽  
Neonatal Infection ◽  
Intercellular Adhesion ◽  
Interleukin 8 ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Reactive Protein ◽  
Factor Α

1. Infection in the neonatal period is difficult to diagnose and is a significant cause of morbidity and mortality in preterm infants. 2. We investigated prospectively the predictive value of plasma measurement of bacterial endotoxin (lipo-polysaccharide), tumour necrosis factor-α, interleukin-6, interleukin-8, intercellular adhesion molecule-1 and C-reactive protein in 60 consecutive newborn infants suspected of having neonatal infection. Plasma samples were taken at the time of acute clinical deterioration. Sixty-two cord blood samples were studied as controls taken at elective Caesarean section. 3. Forty-three infants had confirmed infections, 25 with positive blood cultures. Tumour necrosis factor-α and bacterial endotoxin levels were not significantly elevated over controls, whereas interleukin-6, interleukin-8 and intercellular adhesion molecule-1 levels were all significantly increased in the infected group compared with controls (all P < 0.001). 4. Increased plasma intercellular adhesion molecule-1 levels were a highly sensitive (88%) indicator of clinical infection and were independent of C-reactive protein. Use of these two assays in combination improved the diagnostic sensitivity to 95% and gave a negative predictive value of 97%. Addition of interleukin-6 or interleukin-8 measurements failed to further significantly enhance the prediction of infection. 5. Measurement of intercellular adhesion molecule-1 level may have a clinical role in rapidly confirming, or predicting, the likely diagnosis in cases of suspected neonatal infection.

Control of islet intercellular adhesion molecule-1 expression by interferon-alpha and hypoxia

Diabetes ◽  
1996 ◽  
Vol 45 (10) ◽  
pp. 1336-1343 ◽  
Author(s):  
D. Chakrabarti ◽  
X. Huang ◽  
J. Beck ◽  
J. Henrich ◽  
N. McFarland ◽  
...  
Keyword(s):  
Interferon Alpha ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1

Piperine from Black Pepper Decreased the Expression of Intercellular Adhesion Molecule-1 in Macrophages

2020 ◽  
Vol 19 ◽  
Author(s):  
Nasser Gholijani ◽  
Esmaeil Hashemi ◽  
Zahra Amirghofran
Keyword(s):  
Flow Cytometry ◽  
Adhesion Molecule ◽  
Macrophage Polarization ◽  
Geometric Mean ◽  
Black Pepper ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Therapeutic Benefits ◽  
Relative Fold Change

Background: Macrophages are the main players involved in inflammation. Intercellular adhesion molecule-1 (ICAM-1) facilitates macrophage polarization prior to extravasation into inflamed tissue. Piperine a natural product derived from black pepper possess useful biological and pharmacological activities. In current study, the possible anti-inflammatory effect of piperine on the expression of ICAM-1 on J774.1 murine macrophage cell line was investigated. Methods: Lipopolysaccharide (LPS)-stimulated J774.1 cells were cultured in the presence of different concentrations of piperine to examine the changes in ICAM-1 expression by real-time PCR and flow cytometry. Results: We found that piperine decreased ICAM-1 gene expression level from 2.4 ± 0.25 RFC (relative fold change) in LPS-only treated cells to 0.85 ± 0.525 RFC at 1μg/ml (p<0.05), 0.43 ± 0.27 RFC at 10μg/ml (p<0.01), and 0.26 ± 0.25 RFC at 20μg/ml (p<0.01). In flow cytometry, piperine at all concentrations significantly decreased ICAM-1 surface expressions (P<0.05). The geometric mean fluorescence intensity (g-MFI) in LPS-only treated cells (792 ± 57.3) decreased to 482±70 gMFI at 20 µg/ml piperine. Conclusion: According to the results of this study, by decreasing the expression of ICAM-1, piperine is suggested as a candidate to reduce inflammation and has the potential for therapeutic benefits for immune-mediated diseases.

Sign in / Sign up

Export Citation Format

Share Document