scholarly journals A Pathogenic Isolate of Monopartite PepYLCV DNA A-like Genome Differs Significantly in C1 Gene and CR Sequence, but not in their other Genes

2016 ◽  
Vol 15 (4) ◽  
pp. 124-134 ◽  
Author(s):  
Jamsari Jamsari ◽  
Istino Ferita ◽  
Ade Noverta ◽  
Eko Dharma Husada ◽  
Friedrich W. Herberg ◽  
...  
Keyword(s):  
Pathogenic Isolate

scholarly journals Draft Genome Sequence of Bacillus cereus LA2007, a Human-Pathogenic Isolate Harboring Anthrax-Like Plasmids

2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Angela Pena-Gonzalez ◽  
Chung K. Marston ◽  
Luis M. Rodriguez-R ◽  
Cari B. Kolton ◽  
Julia Garcia-Diaz ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genome Sequence ◽  
Genome Analysis ◽  
Draft Genome ◽  
Anthrax Toxin ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Pneumonia Case ◽  
Pathogenic Isolate

ABSTRACT We present the genome sequence of Bacillus cereus LA2007, a strain isolated in 2007 from a fatal pneumonia case in Louisiana. Sequence-based genome analysis revealed that LA2007 carries a plasmid highly similar to Bacillus anthracis pXO1, including the genes responsible for the production and regulation of anthrax toxin.

scholarly journals Duplicated genes within the variable right end of the genome of a pathogenic isolate of African swine fever virus

1993 ◽  
Vol 74 (10) ◽  
pp. 2125-2130 ◽  
Author(s):  
S. Vydelingum ◽  
S. A. Baylis ◽  
C. Bristow ◽  
G. L. Smith ◽  
L. K. Dixon
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Duplicated Genes ◽  
Pathogenic Isolate

scholarly journals Genomic characterization of a pathogenic isolate of Saccharomyces cerevisiae reveals an extensive and dynamic landscape of structural variation

2021 ◽  
Author(s):  
Lydia R. Heasley ◽  
Juan Lucas Argueso
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Structural Variation ◽  
Genome Structure ◽  
Genomic Variation ◽  
Whole Genome Sequencing Data ◽  
Structural Genomic ◽  
Pathogenic Isolate ◽  
A Genome ◽  
Long Read

The budding yeast Saccharomyces cerevisiae has been extensively characterized for many decades and is a critical resource for the study of numerous facets of eukaryotic biology. Recently, the analysis of whole genome sequencing data from over 1000 natural isolates of S. cerevisiae has provided critical insights into the evolutionary landscape of this species by revealing a population structure comprised of numerous genomically diverse lineages. These survey-level analyses have been largely devoid of structural genomic information, mainly because short read sequencing is not suitable for detailed characterization of genomic architecture. Consequently, we still lack a complete perspective of the genomic variation the exists within the species. Single molecule long read sequencing technologies, such as Oxford Nanopore and PacBio, provide sequencing-based approaches with which to rigorously define the structure of a genome, and have empowered yeast geneticists to explore this poorly described realm of eukaryotic genomics. Here, we present the comprehensive genomic structural analysis of a pathogenic isolate of S. cerevisiae, YJM311. We used long read sequence analysis to construct a haplotype-phased, telomere-to-telomere length assembly of the YJM311 diploid genome and characterized the structural variations (SVs) therein. We discovered that the genome of YJM311 contains significant intragenomic structural variation, some of which imparts notable consequences to the genomic stability and developmental biology of the strain. Collectively, we outline a new methodology for creating accurate haplotype-phased genome assemblies and highlight how such genomic analyses can define the structural architectures of S. cerevisiae isolates. It is our hope that through continued structural characterization of S. cerevisiae genomes, such as we have reported here for YJM311, we will comprehensively advance our understanding of eukaryotic genome structure-function relationships, structural diversity, and evolution.

scholarly journals Comparison of the genome sequences of non-pathogenic and pathogenic African swine fever virus isolates

2008 ◽  
Vol 89 (2) ◽  
pp. 397-408 ◽  
Author(s):  
David A. G. Chapman ◽  
Vasily Tcherepanov ◽  
Chris Upton ◽  
Linda K. Dixon
Keyword(s):  
Virus Assembly ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Open Reading Frames ◽  
Structural Protein ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Pathogenic Isolate ◽  
Virus Isolates

The genomic coding sequences, apart from the inverted terminal repeats and cross-links, have been determined for two African swine fever virus (ASFV) isolates from the same virus genotype, a non-pathogenic isolate from Portugal, OURT88/3, and a highly pathogenic isolate from West Africa, Benin 97/1. These genome sequences were annotated and compared with that of a tissue culture-adapted isolate, BA71V. The genomes range in length between 170 and 182 kbp and encode between 151 and 157 open reading frames (ORFs). Compared to the Benin 97/1 isolate, the OURT88/3 and BA71V isolates have deletions of 8–10 kbp that encode six copies of the multigene family (MGF) 360 and either one MGF 505/530 copy in the BA71V or two copies in the OURT88/3 isolate. The BA71V isolate has a deletion, close to the right end of the genome, of 3 kbp compared with the other isolates. The five ORFs in this region include an additional copy of an ORF similar to that encoding the p22 virus structural protein. The OURT88/3 isolate has interruptions in ORFs that encode a CD2-like and a C-type lectin protein. Variation between the genomes is observed in the number of copies of five different MGFs. The 109 non-duplicated ORFs conserved in the three genomes encode proteins involved in virus replication, virus assembly and modulation of the host's defences. These results provide information concerning the genetic variability of African swine fever virus isolates that differ in pathogenicity.

scholarly journals Mycotic Pneumonia and Placentitis Caused by Mortierella wolfii I. Experimental Infections in Cattle and Sheep

1972 ◽  
Vol 9 (2) ◽  
pp. 131-141 ◽  
Author(s):  
D. O. Cordes ◽  
Margaret E. di Menna ◽  
Margery E. Carter
Keyword(s):  
Oral Administration ◽  
Aspergillus Fumigatus ◽  
Intravenous Administration ◽  
Intravenous Injections ◽  
Experimental Infections ◽  
Pathogenic Isolate ◽  
Large Numbers ◽  
Pregnant Sheep ◽  
Intravenous Inoculation ◽  
Cattle And Sheep

Five million spores of a pathogenic isolate of Mortierella wolfii, when inoculated intravenously into pregnant and nonpregnant cows, caused an acute mycotic pneumonia that resulted in death in less than 4 days. Doses of between 2.5 × 103 and 1 × 105 spores injected intravenously caused mycotic abortion in 16–34 days at 6–8 months of pregnancy. Doses of this order also caused focal, nonfatal pneumonia. Acute mycotic pneumonia was also induced in 6-month-old calves by intravenous injections of spores. Intratracheal, subcutaneous, and oral administration of M. wolfii spores did not produce lesions in cattle. Intravenous inoculation of cattle with up to 1.2 × 108 spores of Absidia ramosa and with 1.2 × 108 spores of Aspergillus fumigatus did not cause lesions. Intravenous administration of large numbers of spores of M. wolfil, A. fumigatus and A. ramosa to pregnant sheep did not cause lesions.

scholarly journals Identification of a New Iron-Regulated Virulence Gene,ireA, in an Extraintestinal Pathogenic Isolate ofEscherichia coli

2001 ◽  
Vol 69 (10) ◽  
pp. 6209-6216 ◽  
Author(s):  
Thomas A. Russo ◽  
Ulrike B. Carlino ◽  
James R. Johnson
Keyword(s):  
Human Urine ◽  
Ex Vivo ◽  
Iron Acquisition ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Virulence Gene ◽  
Luria Bertani ◽  
Infection Model ◽  
Reading Frame ◽  
Pathogenic Isolate

ABSTRACT Our laboratory is studying an extraintestinal pathogenic isolate ofEscherichia coli (CP9) as a model pathogen. We have been using human urine, ascites, and blood ex vivo to identify genes with increased expression in these media relative to expression in Luria-Bertani (LB) broth. Such genes may represent new or unrecognized virulence traits. In this study, we report the identification of a new gene, ireA (iron-responsive element). This gene has an open reading frame of 2,049 nucleotides, and its peptide has a molecular mass of 75.3 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its expression is increased a mean of 3.6-fold in human urine, 16.2-fold in human ascites, and 6.6-fold in human blood relative to expression in LB medium, and it is Fe repressible. IreA also exhibits peptide similarities (48 to 56%) to previously identified proteins that function as siderophore receptors, suggesting that IreA is involved in iron acquisition. PCR-based analysis ofireA's phylogenetic distribution detectedireA in none (0%) of 14 fecal isolates that represented probable commensal strains, but in 13 (26%) of 50 random urine and blood clinical isolates (P = 0.05) and in 5 (100%) of 5 representatives of the J96-like, clonal group of which CP9 is a member (P < 0.001). In a mouse urinary tract infection model, the presence of ireA contributed significantly to CP9's ability to colonize the bladder (P < 0.02), evidence that IreA is a urovirulence factor. Taken together, these findings demonstrate thatireA encodes a new virulence factor, which is likely involved in Fe acquisition.

Molecular Characterization by LSSP-PCR and DNA Sequencing of a Pathogenic Isolate of Leptospira interrogans from Brazil

2012 ◽  
Vol 59 (6) ◽  
pp. 379-388 ◽  
Author(s):  
M. R. V. Cosate ◽  
A. S. Barouni ◽  
E. C. Moreira ◽  
I. F. Veloso ◽  
M. T. R. Gomes ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Molecular Characterization ◽  
Leptospira Interrogans ◽  
Pathogenic Isolate

scholarly journals Vaccination with an Inactivated Virulent Feline Immunodeficiency Virus Engineered To Express High Levels of Env

2005 ◽  
Vol 79 (3) ◽  
pp. 1954-1957 ◽  
Author(s):  
Margaret J. Hosie ◽  
Dieter Klein ◽  
James Μ. Binley ◽  
Thomas H. Dunsford ◽  
Oswald Jarrett ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Virus Vaccine ◽  
Feline Immunodeficiency Virus ◽  
Envelope Glycoprotein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sorting Signal ◽  
Pathogenic Isolate ◽  
Immunodeficiency Virus ◽  
Endocytic Sorting

ABSTRACT An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein, increasing its expression on virions. Cats immunized with inactivated preparations of this modified virus exhibited strong titers of antibody to Env by enzyme-linked immunosorbent assay. Evidence of protection following challenge demonstrated the potential of this approach to lentiviral vaccination.

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