Management of Cucumber Wilt Disease by Bacillus subtilis B006 Through Suppression of Fusarium oxysporum f. sp. cucumerinum in Rhizosphere

2014 ◽  
Vol 13 (3) ◽  
pp. 160-166 ◽  
Author(s):  
Qi-Yao Yang ◽  
Ke Jia ◽  
Wen-Yi Geng ◽  
Rong-Jun Guo ◽  
Shi-Dong Li
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Wilt Disease

scholarly journals Biopestisida Organik Berbahan Aktif Bacillus subtilis dan Pseudomonas fluorescens untuk Mengendalikan Penyakit Layu Fusarium pada Anyelir

2016 ◽  
Vol 21 (2) ◽  
pp. 152
Author(s):  
Hanudin ◽  
Waqiah Nuryani ◽  
Evi Silvia Yusuf ◽  
Budi Marwoto
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Block Design ◽  
Ornamental Plants ◽  
Wilt Disease ◽  
Control Measures ◽  
Plant Production ◽  
Cut Flowers ◽  
Randomized Block Design

Anyelir (Dianthus caryophillus  L.) merupakan salah satu jenis tanaman hias yang mempunyai nilai ekonomi tinggi. Penyakit utama yang menyerang tanaman ini ialah layu Fusarium yang disebabkan oleh Fusarium oxysporum f. sp. dianthi dapat menurunkan kualitas dan kuantitas produksi tanaman sekitar 20-60%.  Pengendalian yang selama ini dilakukan oleh petani bertumpu pada penggunaan  pestisida kimia sintetik. Namun penggunaan bahan kimia tersebut tidak mampu mengeradikasi patogen secara sempurna, terutama pada lapisan tanah yang agak dalam. Salah satu cara pengendalian berwawasan lingkungan ialah menggunakan musuh alami.  Penelitian ini dilaksanakan di Laboratorium dan Rumah Kaca Balai Penelitian Tanaman Hias (1.100 m dpl.), sejak Mei sampai Desember 2009. Penelitian bertujuan mengetahui pengaruh Bacillus subtilis dan Pseudomonas  fluorescens yang diformulasi dalam bentuk biopestisida organik cair dalam pengendalian layu Fusarium pada tanaman anyelir. Rancangan yang digunakan ialah acak  kelompok dengan 10 perlakuan, yaitu 10% ekstrak kascing + 10% molase + B. subtilils + P. fluarescens (BP) dan 10% ekstrak pupuk kandang kuda + 10% molase + BP masing-masing konsentrasi 0,1, 0,3, 0,5, dan 0,7%, dazomet 0,2%, serta kontrol (air ledeng), dengan empat ulangan. Hasil penelitian menunjukkan bahwa populasi bakteri antagonis setelah dilakukan proses fermentasi selama 3 minggu,  meningkat dibandingkan sebelum fermentasi.  Rerata populasi awal sebelum fermentasi bakteri antagonis  107-109 cfu/ml meningkat menjadi 1010-1012 cfu/ml pada 3 minggu setelah fermentasi. Populasi kedua agens biokontrol tersebut setelah penyimpanan selama 2 bulan cenderung stabil berkisar antara 1010-1011 cfu/ml.  Perlakuan B. subtilis dan P. fluorescens  yang disuspensikan ke dalam ekstrak kascing + molase pada taraf konsentrasi 0,5% kemudian difermentasikan dalam biofermentor selama 3 minggu secara konsisten dapat menekan serangan F. oxysporum f. sp. dianthi pada anyelir. Implikasi hasil penelitian ini dapat meningkatkan daya saing komoditas tanaman hias melalui pemanfaatan sumber daya alam nasional secara optimal berkelanjutan untuk mendukung industri tanaman hias yang berdaya saing tinggi.<br /><br />Carnation (Dianthus caryophillus  L.) is one of the most economically important cut flowers in Indonesia. The crops is commonly cultivated in the highland areas of the country. Cultivations of the crops in the production center areas have faced various problems, especially wilt disease caused by Fusarium oxysporum  f. sp. dianthi as the most important one. Based on the field observation it is known that the disease could reduce plant production and its yield quality up to 20-60%. To control the disease, farmers usually use a synthetic chemical pesticides.  However the control measures are not sufficiently effective to overcome the diseases problems.  Therefore, an alternative control measures which are more environmentally friendly is necessary. The use of biocontrol agents is nowdays bring popular to be recommended to  control the disease.  A study on the control of fusarial wilt disease on carnation was carried out  in the Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute (1,100 m asl.) from May to December 2009, using Bacillus subtilis and Pseudomonas  fluorescens  formulated in the liquid organic pesticide.  The study was arranged in a randomized block design, with 10 treatments i.e. 10% vermi compost + 10% molase + BP and 10% horse manure + 10% molase + BP consentration 0.1, 0.3, 0.5, 0.7% resfectively, dazomet 0,2% and control with four replications. The results showed that population of antagonistic bacterial was increased from  107-109  to 1010-1012 cfu/ml  after 3 weeks fermentation in the organic carrier.  The population of two antagonistic bacteria  was likely stable on 1010-1011 cfu/ml after storing 2 months. The treatments of B. subtilis and P.  fluorescens suspended in the vermi compost extract and molases on the concentration level of  0.5% and formulated in the biofermentor for 3 weeks  were consistenly effective in reducing Fusarium wilt on carnation. The implication of research results could be increase commodity competitive ability of ornamental plants by using national nature resource on a continuity for support the ornamental plants industry with high competitiveness.<br /><br />

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Biocontrol of Fusarium wilt disease in muskmelon with Bacillus subtilis Y-IVI

BioControl ◽  
2012 ◽  
Vol 58 (2) ◽  
pp. 283-292 ◽  
Author(s):  
Qingyun Zhao ◽  
Wei Ran ◽  
Hui Wang ◽  
Xiang Li ◽  
Qirong Shen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Fusarium Wilt Disease

Vascular Wilt of Common Naranjilla (Solanum quitoense) Caused by Fusarium oxysporum in Ecuador

2001 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
J. B. Ochoa ◽  
B. Yangari ◽  
V. Galarza ◽  
J. Fiallos ◽  
M. A. Ellis
Keyword(s):  
Fusarium Oxysporum ◽  
Wilt Disease ◽  
Vascular Wilt ◽  
Major Constraint ◽  
Vascular Wilt Disease

Farmers have generally abandoned production of “common naranjilla” in many areas mainly due to uncontrollable epidemics of an apparent vascular wilt disease. Naranjilla vascular wilt (NVW) and is currently the major constraint to the production of naranjilla in Ecuador, where losses due to NVW may reach up to 80%. Accepted for publication 13 September 2001. Published 18 September 2001.

scholarly journals Immobilized Bacillus subtilis by ionic gelation as biocontrol alternative of Fusarium oxysporum f.sp. lycopersici

2018 ◽  
Author(s):  
Castañeda Alvarez Estefania ◽  
Sánchez Leal Ligia
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Sodium Alginate ◽  
Ionic Gelation ◽  
Cellular Viability ◽  
Market Demand ◽  
Control Activity ◽  
Pests And Diseases

For farmers the use of agrochemicals is the preferred method to control pests and diseases. Considering the market demand for biological control products, the encapsulation could be a competent alternative to current commercial formulations for cellular viability and controlled release. The purpose of this study was to use ionic gelation with sodium alginate, starch and maltodextrin to immobilize Bacillus subtilis and to evaluate the biocontrol effect against Fusarium oxysporum f. sp. lycopersici in vitro. The matrix with a concentration of 2% sodium alginate, 1% starch, and 1% maltodextrin is a suitable method for cellular viability and biological control activity against Fusarium oxysporum f. sp. lycopersici, with a reduction of mycelial growth of 49.6% and a survival rate for Bacillus subtilis of 98.05% (p less than 0.0001).The use of immobilized bacteria as biological control agents are sustainable and effective bio-inputs that could be used at industrial scale and benefit the tomato crops against attack by Fusarium oxysporum f. sp. lycopersici.

scholarly journals Bacillus spp.; perspectiva de su efecto biocontrolador mediante antibiosis en cultivos afectados por fitopatógenos

Nova ◽  
2011 ◽  
Vol 9 (16) ◽  
pp. 177 ◽  
Author(s):  
Cristian Layton ◽  
Edna Maldonado ◽  
Luisa Monroy ◽  
Lucía Constanza Corrales Ramírez MSC ◽  
Ligia Consuelo Sánchez Leal MSC
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Bacillus Sp ◽  
Bacillus Brevis ◽  
Iturin A ◽  
Bacillus Spp

El presente estudio documental evalúa el efecto biocontrolador del género <em>Bacillus sp </em>contra hongos fitopatógenos de plantas, particularmente, a través de relaciones antagónicas inductoras de muerte celular en términos inminentemente naturales. <em>Fusarium oxysporum </em>se encuentra muy relacionado con casos de marchitez vascular y pudrición de raíz en variedad de plantas, obstrucción de los vasos que permiten la circulación vegetal hasta causar amarillamiento de las hojas por imposibilidad en el transporte de nutrientes, causal de grandes pérdidas económicas en el campo agrícola nacional. Se han establecido varios mecanismos para controlar este hongo micelial dentro de las que se encuentran el uso extensivo y variable de agroquímicos y pesticidas, práctica que por sus efectos nocivos con el medio ambiente se ha comenzado a reemplazar por empleo de especies del género <em>Bacillus. </em>La acción biocontroladora de este género bacteriano esta mediada por su perfil bioquímico ya que son productores de múltiples metabolitos biológicamente activos, en el caso de <em>Bacillus subtilis </em>de Iturin A y fengycin y en <em>Bacillus brevis de gramicidina S </em>(1-5) son capaces de inhibir el desarrollo y crecimiento normal de otros microorganismos, lo que sugiere su utilización para el biocontrol de plagas en aras al fortalecimiento de los actuales estándares de calidad en los procesos ambientales.

scholarly journals Uji masa simpan pelet Trichoderma harzianum dan kemampuannya dalam menghambat perkembangan penyakit Layu Fusarium pada bibit tomat.

2018 ◽  
Vol 3 (2) ◽  
pp. 117-127
Author(s):  
Rizka Musfirah ◽  
Rina Sriwati ◽  
Tjut Chamzurni
Keyword(s):  
Vitamin A ◽  
Shelf Life ◽  
Fusarium Oxysporum ◽  
Solanum Lycopersicum ◽  
Plant Height ◽  
Incubation Period ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Wilt Disease ◽  
Damping Off

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum  4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.

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