Biological Species in the Gibberella fujikuroi Species Complex Isolated from Maize Kernels in Argentina

2006 ◽  
Vol 5 (3) ◽  
pp. 350-355 ◽  
Author(s):  
Maria M. Reynoso . ◽  
Adriana M. Torres . ◽  
Sofia N. Chulze .
Keyword(s):  
Species Complex ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Gibberella Fujikuroi Species Complex ◽  
Maize Kernels

scholarly journals Gibberella xylarioides Sensu Lato from Coffea canephora: a New Mating Population in the Gibberella fujikuroi Species Complex

2005 ◽  
Vol 71 (12) ◽  
pp. 8466-8471 ◽  
Author(s):  
Pascale C. E. Lepoint ◽  
Françoise T. J. Munaut ◽  
Henri M. M. Maraite
Keyword(s):  
Mating Type ◽  
Species Complex ◽  
Sequence Data ◽  
Coffea Canephora ◽  
Biological Species ◽  
West African ◽  
Gibberella Fujikuroi ◽  
Translation Elongation ◽  
Gibberella Fujikuroi Species Complex

ABSTRACT Gibberella xylarioides Heim & Saccas (presumed anamorph, Fusarium xylarioides Steyaert) is the causal agent of coffee wilt disease, an economically important tracheomycosis in Africa. In vitro crosses carried out with Congolese, Ugandan, and Tanzanian single-ascospore/conidial isolates originating from diseased Coffea canephora/excelsa demonstrated a heterothallic mating system, controlled by a single locus with two alleles, MAT-1 and MAT-2. Compatible isolates produced fertile perithecia within 2 to 8 weeks after mating. Mating type (MAT) was characterized by PCR with primer pairs previously developed for the Gibberella fujikuroi species complex (GFC) and for Fusarium oxysporum. All strains analyzed were morphologically identical and corresponded to Booth's description of the “female” F. xylarioides strain. Based on crossing results and MAT-2/translation elongation 1-α (tef) sequence data, G. xylarioides, as currently understood, is demonstrated to encompass at least three “groups”: G. xylarioides sensu strictu Ia, defined hitherto by two “historical” West African strains originating from the severe 1930s to 1950s epidemic (CBS 25852 and CBS 74979); G. xylarioides sensu strictu Ib, defined by two “historical” Central African lowland strains (DSMZ 62457 and ATCC 15664); and G. xylarioides sensu lato II, containing Congolese, Ugandan, and Tanzanian C. canephora/excelsa isolates. Infertility of crosses between the coffee wilt pathogen and known GFC mating populations demonstrates that G. xylarioides sensu lato constitutes a new biological species within the G. fujikuroi complex. MUCL 44532/MUCL 43887 and MUCL 35223/MUCL 44549 are proposed as G. xylarioides sensu lato II MAT-1/MAT-2 reference mating type tester strains.

scholarly journals PCR-Based Identification of MAT-1 andMAT-2 in the Gibberella fujikuroi Species Complex

2000 ◽  
Vol 66 (10) ◽  
pp. 4378-4382 ◽  
Author(s):  
Emma T. Steenkamp ◽  
Brenda D. Wingfield ◽  
Teresa A. Coutinho ◽  
Kurt A. Zeller ◽  
Michael J. Wingfield ◽  
...  
Keyword(s):  
Species Complex ◽  
Fusarium Verticillioides ◽  
Fusarium Culmorum ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Additional Species ◽  
Individual Mating ◽  
Sexual Cross ◽  
Phylogenetic Lineages ◽  
Gibberella Fujikuroi Species Complex

ABSTRACT All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphsMAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an ∼800-bp fragment from MAT-2 or an ∼200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, andFusarium culmorum, which have, or are expected to have,Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and otherFusarium species.

scholarly journals Molecular Standardization of Mating Type Terminology in the Gibberella fujikuroi Species Complex

1999 ◽  
Vol 65 (9) ◽  
pp. 4071-4076 ◽  
Author(s):  
Z. Kerényi ◽  
K. Zeller ◽  
L. Hornok ◽  
J. F. Leslie
Keyword(s):  
Dna Sequence ◽  
Mating Type ◽  
Species Complex ◽  
Pcr Primers ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Gibberella Fujikuroi Species Complex ◽  
Sexual Crosses ◽  
Standard Tester ◽  
Sequence Similarities

ABSTRACT Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and is usually identified following sexual crosses with standard, female-fertile tester isolates. The mating type alleles have been arbitrarily designated “+” and “−” within each biological species, and the nomenclature is tied to the standard tester strains. We developed a pair of PCR primers that can be used to amplify a unique fragment of one of the mating type alleles (MAT-2) from at least seven of the biological species in this species complex. Based on the amplification pattern, we propose a replacement for the existing, arbitrary +/− terminology that is presently in use. The new terminology is based on DNA sequence similarities between the mating type allele fragments from the biological species of the G. fujikuroi species complex and the corresponding fragments from other filamentous ascomycetes.

Molecular Systematics and Phylogeography of the Gibberella fujikuroi Species Complex

Mycologia ◽  
1998 ◽  
Vol 90 (3) ◽  
pp. 465 ◽  
Author(s):  
Kerry O'Donnell ◽  
Elizabeth Cigelnik ◽  
Helgard I. Nirenberg
Keyword(s):  
Molecular Systematics ◽  
Species Complex ◽  
Gibberella Fujikuroi ◽  
Gibberella Fujikuroi Species Complex

scholarly journals Toxigenicity of Fusarium Species in Gibberella fujikuroi Species Complex (GFSC) Associated With Stalk and Ear Rot Disease of Corn

2013 ◽  
Vol 2 (3) ◽  
pp. 147-154 ◽  
Author(s):  
Titi Darnetty ◽  
Baharuddin Salleh
Keyword(s):  
Species Complex ◽  
Fusarium Species ◽  
Fumonisin B1 ◽  
Ultra Performance Liquid Chromatography ◽  
Gibberella Fujikuroi ◽  
Ear Rot ◽  
Fusarium Spp ◽  
Low Levels ◽  
Rot Disease ◽  
Gibberella Fujikuroi Species Complex

Fusarium stalk and ear rot disease did not only cause significant losses of yield but also produced mycotoxins that are harmful to animals and human. This study was conducted to elucidate three major mycotoxins i.e. fumonisin B1 (FUMB1), moniliformin (MON), and beauvericin (BEA) produced by the Fusarium spp. isolated from corn showing typical stalk and ear rot symptoms in Indonesia, Malaysia and Thailand. Twenty selected strains of Fusarium species in Gibberella fujikuroi species complex i.e. F.verticillioides, F. proliferatum, F. subglutinans, and F. konzum were analyzed for production of the three mycotoxins by using an Ultra Performance Liquid Chromatography (UPLC).  All strains of F. verticillioides and F. proliferatum produced FUMB1 at high levelsand MON at low levels. Many strains of F. verticillioides (67%) and F. proliferatum (50%) did not produce BEA while the others produced BEA at low levels. Two strains of F. subglutinans did not produce FUMB1 but produced MON at low levels. One strain of F. subglutinans produced BEA and the other one did not produce the toxin.  Two strains of F. konzum produced both MON and BEA at low levels but only one strain produced FUMB1 at a low level. These mycotoxins have not been reported from Fusarium spp. in Gibberella fujikuroi species complex isolated form stalk and ear rot diseases of corn in these areas. Therefore, concerted efforts must be made to educate all stake holders about the presence and health hazards of these mycotoxins.

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