High Vero Cell Density and Rabies Virus Proliferation on Fibracel Disks Versus Cytodex-1 in Spinner Flask

2011 ◽  
Vol 14 (7) ◽  
pp. 441-448 ◽  
Author(s):  
S. Mehdi Hassanzade ◽  
Ali Zavareh ◽  
M. Ali Shokrgozar ◽  
Ali Ramezani ◽  
Ahmad Fayaz
Keyword(s):  
Vero Cell ◽  
Cell Density ◽  
Rabies Virus ◽  
Spinner Flask

Rabies virus production in high vero cell density cultures on macroporous microcarriers

2004 ◽  
Vol 85 (5) ◽  
pp. 506-515 ◽  
Author(s):  
A. Y. Yokomizo ◽  
M. M. Antoniazzi ◽  
P. L. Galdino ◽  
N. Azambuja ◽  
S. A. C. Jorge ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Density ◽  
Rabies Virus ◽  
Virus Production ◽  
Macroporous Microcarriers

Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

2020 ◽  
Vol 35 (4) ◽  
pp. 237-242
Author(s):  
Ya. M. Krasnov ◽  
Zh. V. Alkhova ◽  
S. V. Generalov ◽  
I. V. Tuchkov ◽  
E. A. Naryshkina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Vero Cell ◽  
Whole Genome Sequencing ◽  
Cell Line ◽  
Rabies Virus ◽  
Genome Sequencing ◽  
Virus Strain ◽  
Whole Genome ◽  
Vero Cell Line ◽  
Rabies Virus Strain

scholarly journals Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation (“Moscow 3253” Strain) in Vero Cell Culture

2014 ◽  
pp. 101-103
Author(s):  
S. V. Generalov ◽  
E. G. Abramova ◽  
Zh. V. Matveeva ◽  
I. M. Zhulidov ◽  
L. V. Savitskaya ◽  
...  
Keyword(s):  
Cell Culture ◽  
Vero Cell ◽  
Rabies Virus ◽  
Vero Cell Culture

scholarly journals Optimization of environment for high density Vero cell culture: effect of dissolved oxygen and nutrient supply on cell growth and changes in metabolites

1986 ◽  
Vol 81 (1) ◽  
pp. 65-103
Author(s):  
A.T. Nahapetian ◽  
J.N. Thomas ◽  
W.G. Thilly
Keyword(s):  
Cell Growth ◽  
Dissolved Oxygen ◽  
Vero Cell ◽  
Cell Density ◽  
Lactate Production ◽  
Nutrient Supply ◽  
High Density ◽  
Limiting Factors ◽  
Utilization Rate ◽  
Maximum Cell Density

This study was initiated for optimization of the environment of a technologically useful mammalian cell line for high density production. Cultures of Vero cells on microcarriers were perfused with 100%, 50%, 25% and 12.5% modified L15 media (galactose was replaced with 10 mM-fructose, with 4 mM-glutamine and 5% foetal bovine serum) in phosphate-buffered saline at either 4 or 8 vol. day-1. Cell growth, pH, dissolved oxygen, and changes in the metabolites, lactate to pyruvate and lactate to ammonia indices, demonstrated that under the conditions used in the present study, perfusion of cultures with 50% L15 medium in PBS at 8 vol. day-1 provided the optimum microenvironment for Vero cell growth. The highest cell density in the perfused cultures was 3 X 10(7) cells ml-1, which at these conditions was ten times higher than the maximum cell density (3 X 10(6) cells ml-1) obtained in a batch culture. Nutrient supply and conditioning factors were the most probable growth-limiting factors in cultures that were perfused with 12.5% and 25% L15 media, while multilayering, limitation of available oxygen, and accumulation of metabolic end products in the cellular microenvironment were the most probable causes of a density-dependent inhibition of cell growth observed under the optimized and overfed (supply of 100% L15 medium at the rate of 8 vol. day-1) culture conditions. Under the optimized environmental condition, the major source of energy was probably glutamine during the first week. However, significant utilization of fructose became evident at higher cell densities during the second week, when lactate production dramatically declined and reached an almost undetectable level, while respiration progressively assumed the predominant role in energy production. It is postulated that ‘available’ oxygen in the multicell-layered microenvironment of the optimized cultures was higher than in the overfed culture due to the greater utilization rate of oxygen for oxidation of excess nutrients in the overfed culture.

scholarly journals Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes

2012 ◽  
Vol 64 (4) ◽  
pp. 429-441 ◽  
Author(s):  
Emma Petiot ◽  
Amal El-Wajgali ◽  
Geoffrey Esteban ◽  
Cécile Gény ◽  
Hervé Pinton ◽  
...  
Keyword(s):  
Vero Cell ◽  
Real Time ◽  
Cell Density ◽  
Real Time Monitoring

scholarly journals Purified Vero Cell Rabies Vaccine (PVRV, Verorab®): A Systematic Review of Intradermal Use Between 1985 and 2019

2020 ◽  
Vol 5 (1) ◽  
pp. 40 ◽  
Author(s):  
Thomas Moulenat ◽  
Céline Petit ◽  
Valérie Bosch Castells ◽  
Guy Houillon
Keyword(s):  
Systematic Review ◽  
Vero Cell ◽  
Rabies Virus ◽  
Neutralizing Antibodies ◽  
Rabies Vaccine ◽  
Post Exposure Prophylaxis ◽  
Post Exposure ◽  
Exposure Prophylaxis ◽  
Rabies Prevention ◽  
Rabies Vaccination

The purified Vero cell rabies vaccine (PVRV; Verorab®, Sanofi Pasteur) has been used in rabies prevention since 1985. Evolving rabies vaccination trends, including shorter intradermal (ID) regimens with reduced volume, along with WHO recommendation for ID administration has driven recent ID PVRV regimen assessments. Thus, a consolidated review comparing immunogenicity of PVRV ID regimens during pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) is timely and beneficial in identifying gaps in current research. A search of seven databases for studies published from 1985 to November 2019 identified 35 studies. PrEP was assessed in 10 studies (n = 926) with 1–3-site, 1–3-visit regimens of up to 3-months duration. Seroconversion (rabies virus neutralizing antibodies [RVNA] ≥ 0.5 IU/mL) rates of 90–100% were reported within weeks, irrespective of regimen, with robust booster responses at 1 year (100% seroconversion rates by day 14 post-booster). However, data are lacking for the current WHO-recommended, 2-site, 1-week ID PrEP regimen. PEP was assessed in 25 studies (n = 2136) across regimens of 1-week to 90-day duration. All ID PEP regimens assessed induced ≥ 99% seroconversion rates (except in HIV participants) by day 14–28. This review confirms ID PVRV suitability for rabies prophylaxis and highlights the heterogeneity of use in the field.

scholarly journals Serological profiling of rabies antibodies by enzyme-linked immunosorbent assay and its comparative analysis with rapid fluorescent focus inhibition test in mouse model

2019 ◽  
Vol 12 (1) ◽  
pp. 126-130 ◽  
Author(s):  
Ashis Debnath ◽  
Dinesh C. Pathak ◽  
Narayan Ramamurthy ◽  
Gulam Mohd ◽  
A. B. Pandey ◽  
...  
Keyword(s):  
Vero Cell ◽  
Rabies Virus ◽  
Immunosorbent Assay ◽  
Disease Transmission ◽  
Regression Line ◽  
Rabies Vaccine ◽  
Inhibition Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mice Model ◽  
Fluorescent Focus

Aim: In this study, we have used enzyme-linked immunosorbent assay (ELISA) as an alternative test to replace the cumbersome rapid fluorescent focus inhibition test (RFFIT) to ascertain the immune status of immunized mice against rabies virus. Materials and Methods: Rabies is a devastating disease worldwide caused by rabies virus. Proper usage of pre- or post-exposure rabies vaccine can prevent the disease transmission. In this study, mice were immunized with Vero cell-adapted inactivated rabies vaccine. RFFIT was used as a test to determine the serum neutralizing titers in infected/vaccinated mice. Seroprofiling of mice sera was done in vitro by ELISA. Results: Twenty-one days post-immunization, both ELISA and RFFIT assays indicated similar antibody levels in mice sera that were immunized with Vero cell-adapted inactivated rabies vaccine. Both the tests were correlated, and the linearity was verified by the regression line (R2=0.979). Conclusion: In this study, we profiled the serological status of Vero cell-adapted inactivated rabies vaccine through ELISA in mice model that correlated well with the OIE gold standard test RFFIT.

scholarly journals Study on Preparation of New Purified Vero Cell Rabies Vaccine

2019 ◽  
Vol 4 (4) ◽  
Keyword(s):  
Vero Cell ◽  
Rabies Virus ◽  
Rabies Vaccine ◽  
Mass Vaccination ◽  
Vaccine Preventable Disease ◽  
Preventable Disease ◽  
Endemic Areas ◽  
Vaccination Campaigns ◽  
Negative Sense ◽  
Family Rhabdoviridae

Rabies is a 100% vaccine-preventable disease. A cheap, rabies vaccine for humans that could be used in mass vaccination campaigns would be a valuable weapon against rabies. Despite the existence of safe and effective vaccines, rabies disease still causes an estimated more human deaths a year in the endemic areas in Asia and Africa. Rabies virus is single stranded negative sense RNA from genus lyssavirus and _ family rhabdoviridae.

scholarly journals In-situ cell density monitoring and apoptosis detection in adherent Vero cell bioreactor cultures

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Emma Petiot ◽  
Amal El-Wajgali ◽  
Geoffrey Esteban ◽  
Cécile Gény ◽  
Hervé Pinton ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Density ◽  
Bioreactor Cultures ◽  
Apoptosis Detection
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