Ameliorative Potential of Alcoholic Red Wine Against CCl4-induced Renalotoxicity in Albino Rats

E.O. Ibukun; G.O. Oladipo

doi:10.3923/krj.2016.1.8

Effects of Provinols on Cardiodynamics and Coronary Flow in Islodated Rat Hearts

Serbian Journal of Experimental and Clinical Research ◽

10.1515/sjecr-2015-0060 ◽

2016 ◽

Vol 17 (2) ◽

pp. 99-106

Author(s):

Ana Popovic ◽

Olga Pechanova ◽

Radoslava Rehakova ◽

Vladimir Zivkovic ◽

Ivan Srejovic ◽

...

Keyword(s):

Red Wine ◽

Epidemiological Studies ◽

Albino Rats ◽

Alcohol Content ◽

Experimental Conditions ◽

Positive Effects ◽

Rat Hearts ◽

Wide Range ◽

Wistar Albino Rats ◽

Experimental Group

Abstract Provinols are an alcohol-free extract of red wine that contains a wide range of polyphenols. Polyphenols are a group of chemical compounds found in diverse plants. Polyphenols are considered to protect against cardiovascular disease. Although some older epidemiological studies have indicated that the positive effects of red wine on heart disease can be attributed to the alcohol content alone, there is now powerful evidence that polyphenols present in red wine are responsible for these positive effects. The hearts of male Wistar albino rats (n = 36, 12 in each experimental group, 10 weeks old, body mass 250 ± 30 g) were excised and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure (40-120 cmH2O). Parameters of cardiac function (dp/dt max, dp/dt min, SLVP, DLVP, HR, CF) were measured after perfusion with three different concentrations of provinols (5 μg/ml, 10 μg/ml and 50 μg/ml). Administration of the highest dose (50 μg/ml) induced a significant increase in dp/dt max, dp/dt min, HR and CF compared with control conditions at CPP = 40 cmH2O, while an intermediate dose increased dp/dt max at the same CPP. Generally viewed, the results of the present study suggest that provinols may have a beneficial effect on the intact myocardium and coronary circulation. These findings could constitute an important step in further investigation of these polyphenols under different representative experimental conditions in the heart, as well as providing a good basis for potential clinical studies in this field.

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Effects of renovascular hypertension on rat adrenal zona glomerulosa

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083783 ◽

1978 ◽

Vol 36 (2) ◽

pp. 582-583

Author(s):

G. Mazzocchi ◽

P. Rebuffat ◽

C. Robba ◽

P. Vassanelli ◽

G. G. Nussdorfer

Keyword(s):

Renovascular Hypertension ◽

Left Kidney ◽

Renin Angiotensin System ◽

Plasma Renin ◽

Zona Glomerulosa ◽

Ultrastructural Changes ◽

Albino Rats ◽

Left Renal Artery ◽

Angiotensin System ◽

And Control

It is well known that the rat adrenal zona glomerulosa steroidogenic activity is controlled by the renin-angiotensin system. The ultrastructural changes in the rat zona glomerulosa cells induced by renovascular hypertension were described previously, but as far as we are aware no correlated biochemical and morphometric investigations were performed.Twenty adult male albino rats were divided into 2 experimental groups. One group was subjected to restriction of blood flow to the left kidney by the application of a silver clip about the left renal artery. The other group was sham-operated and served as a control. Renovascular hypertension developed in about 10 days: sistolic blood pressure averaged 165 ± 6. 4 mmHg, whereas it was about 110 ± 3. 8 mmHg in the control animals. The hypertensive and control rats were sacrificed 20 days after the operation. The blood was collected and plasma renin activity was determined by radioimmunological methods. The aldosterone concentration was radioimmunologically assayed both in the plasma and in the homogenate of the left capsular adrenal gland.

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Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051451 ◽

1974 ◽

Vol 32 ◽

pp. 288-289

Author(s):

Alfredo Feria-Velasco ◽

Guadalupe Tapia-Arizmendi

Keyword(s):

Fine Structure ◽

Domestic Fowl ◽

Animal Species ◽

Acinar Cells ◽

Harderian Gland ◽

Myoepithelial Cells ◽

The Other ◽

Albino Rats ◽

Adult Rats ◽

Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

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Cell Fine Structure as Revealed in Dessicator-Dried Resinless Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099532 ◽

1981 ◽

Vol 39 ◽

pp. 622-623

Author(s):

R. P. Becker ◽

J. J. Wolosewick ◽

J. Ross-Stanton

Keyword(s):

Fine Structure ◽

Tannic Acid ◽

Three Dimensional ◽

Albino Rats ◽

Cell Organelles ◽

Vascular Perfusion ◽

Thin Sections ◽

Carbon Coated ◽

Embedding Medium ◽

Polyethylene Glycol 6000

Methodology has been introduced recently which allows transmission and scanning electron microscopy of cell fine structure in semi-thin sections unencumbered by an embedding medium. Images obtained from these “resinless” sections show a three-dimensional lattice of microtrabeculfee contiguous with cytoskeletal structures and membrane-bounded cell organelles. Visualization of these structures, especially of the matiiDra-nous components, can be facilitated by employing tannic acid in the fixation step and dessicator drying, as reported here.Albino rats were fixed by vascular perfusion with 2% glutaraldehyde or 1.5% depolymerized paraformaldehyde plus 2.5% glutaraldehyde in 0.1M sodium cacodylate (pH 7.4). Tissues were removed and minced in the fixative and stored overnight in fixative containing 4% tannic acid. The tissues were rinsed in buffer (0.2M cacodylate), exposed to 1% buffered osmium tetroxide, dehydrated in ethyl alcohol, and embedded in pure polyethylene glycol-6000 (PEG). Sections were cut on glass knives with a Sorvall MT-1 microtome and mounted onto poly-L-lysine, formvar-carbon coated grids while submerged in a solution of 95% ethanol containing 5% PEG.

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