Antioxidant Effects of Aqueous Extract of Sweet Potato and Ascorbic Acid on Paracetamol Induced Damage

2005 ◽  
Vol 6 (1) ◽  
pp. 87-92
Author(s):  
A.O. Lawal . ◽  
A. Ologundudu . ◽  
F.O. Obi . ◽  
I.V. Ogungbe . ◽  
A.O. Olakanye . ◽  
...  
Author(s):  
O. O. Otusanya ◽  
A. A. Ogunwole ◽  
M. O. Tijani

The present study evaluated the allelopathic potential of fresh shoot aqueous extracts of Tithonia rotundifolia (FSET) and Murraya koenigii (FSEM) on the germination of seeds, growth, chlorophyll, ascorbic acid and percentage crude protein accumulation of Capsicum annuum. The laboratory experimental results showed that seed germination and juvenile seedling growth of C. annuum were significantly retarded by both FSEM and FSET. The radicle growth was more susceptible to the phytotoxicity of both extracts than the plumule growth. Both plants extract had a concentration-dependent inhibitory effect on the seedling growth of the recipient crop while the FSET was more phytotoxic than FSEM. However, this retardatory effect of aqueous extracts on growth observed in the laboratory was reversed in the soil-cultured experiments such that application of FSEM significantly enhanced all the studied growth parameters (shoot height, number of leaves, leaf area, leaf area ratio, shoot fresh and dry weight, root fresh and dry weight, chlorophyll a, chlorophyll b, total chlorophyll, ascorbic acid content in the shoot and percentage crude protein in the shoot and fruits). Likewise, FSET application significantly increased the leaf area, total chlorophyll and percentage crude protein accumulation in the shoot and fruits of the recipient crop at P<.05. This study then emphasizes the fact that T. rotundifolia and M. koenigii are allelopathic plants. The increased ascorbic acid and percentage crude protein accumulation in the aqueous extract-treated crop could, apart from enhancing the nutritional benefits of the test crop, be an adaptive mechanism evolved by the crop to overcome the allelopathic stress posed by the application of the aqueous extracts. These findings therefore suggest that allelochemicals in the aqueous extract of M. koenigii and T. rotundifolia could serve as biofertilizers for boosting the production of C. annuum.


2019 ◽  
Vol 81 (4) ◽  
Author(s):  
Ulfah Anwar ◽  
Siti Pauliena Mohd Bohari

The aim of this research is to investigate the wound healing process in in vitro by combining the Manihot esculenta aqueous extract and therapeutic ultrasound. Firstly, the optimization seeding densities of HSF cell 1184 in six-well plate, and then followed by the scratch assay experiment. The scratched that made was treated with the remedial treatments (Manihot esculenta aqueous extract only; ascorbic acid+ therapeutic ultrasound; Manihot esculenta aqueous extract+ ascorbic acid; Manihot esculenta aqueous extract+ therapeutic ultrasound and also the combination of these three materials). The rate of wound closure was observed and analysed at a time interval of 0, 2, 4, 6, 8, 10 and 24 h by using image J software. Then, the cells viability were analysed using the MTT assay. The result showed that Manihot esculenta aqueous extract coupled with specific dose therapeutic ultrasound represents a significantly high rate of wound closure at 96.10 % with the cell numbers at 5.44×105 cells/mL when compared to the other combination therapy. The finding of this study revealed that Manihot esculenta aqueous extract 200 µg/mL and the therapeutic ultrasound specific dose (3 MHz, 300 mWatt/cm2, 50% in 5 min) have the potential in accelerating wound healing process of cells in in vitro.


2008 ◽  
Author(s):  
Takahiro Orikasa ◽  
Daisuke Nei ◽  
Poritosh Roy ◽  
Nobutaka Nakamura ◽  
Takeo Shiina ◽  
...  

Author(s):  
S. Sujitha

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.


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