Effect of Bisphenol-A on the Sertoli Cell Culture from Prepubertal Male Wistar Rats

2001 ◽  
Vol 2 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Mohammad A. Awal ◽  
Masamichi Kurohmaru . ◽  
Takuo Mizukami . ◽  
Bibin B. Andriana . ◽  
Yoshiakira Kanai . ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bisphenol A ◽  
Sertoli Cell ◽  
Wistar Rats ◽  
Male Wistar Rats ◽  
Sertoli Cell Culture

Biochemical Effects of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) on the Sertoli Cell Culture from Prepubertal Male Wistar Rats

2001 ◽  
Vol 1 (5) ◽  
pp. 311-315
Author(s):  
M. A. Awal ◽  
M. N. H. Siddiqi . ◽  
M. Kurohmaru . ◽  
B. B. Andriana . ◽  
T. Mizukami . ◽  
...  
Keyword(s):  
Cell Culture ◽  
Sertoli Cell ◽  
Wistar Rats ◽  
Biochemical Effects ◽  
Male Wistar Rats ◽  
Tetrachlorodibenzo P Dioxin ◽  
Sertoli Cell Culture

Molecular changes induced by bisphenol-A in rat Sertoli cell culture

2011 ◽  
Vol 57 (5) ◽  
pp. 228-232 ◽  
Author(s):  
Yoko Izumi ◽  
Kohei Yamaguchi ◽  
Tomomoto Ishikawa ◽  
Makoto Ando ◽  
Koji Chiba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bisphenol A ◽  
Sertoli Cell ◽  
Molecular Changes ◽  
Sertoli Cell Culture

scholarly journals Male Sexual Development in the Nonhuman Primate. III. Sertoli Cell Culture and Age-Related Differences

1983 ◽  
Vol 28 (5) ◽  
pp. 1207-1215 ◽  
Author(s):  
Byung C. Lee ◽  
Jose L. Pineda ◽  
Bessie E. Spiliotis ◽  
Teri J. Brown ◽  
Barry B. Bercu
Keyword(s):  
Cell Culture ◽  
Sertoli Cell ◽  
Nonhuman Primate ◽  
Sexual Development ◽  
Age Related ◽  
Sertoli Cell Culture

scholarly journals Influence of prolonged flaxseed ( Linum usitatissimum ) consumption over epididymis and testicle histoarchitecture of Wistar rats

2017 ◽  
Vol 37 (6) ◽  
pp. 650-656 ◽  
Author(s):  
Lanna B.N.S. Corrêa ◽  
Ludmila F.M. de F. Cardozo ◽  
Ilma C. de A. Ribeiro ◽  
Gilson T. Boaventura ◽  
Maurício A. Chagas
Keyword(s):  
Sertoli Cell ◽  
Wistar Rats ◽  
Serum Levels ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Lactation Period ◽  
Luminal Diameter ◽  
Flaxseed Meal ◽  
Male Wistar Rats ◽  
Hormonal Evaluation

ABSTRACT: Flaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantification showed no significant differences in the FG (P=0.98). These results revealed that the continuous and prolonged intake of 25% flaxseed meals from gestation to 250 days of age, even with a significant increase in serum levels of estradiol, does not exert adverse effects on the testicular and epididymal structure or on the cells participating in the spermatogenesis of rats.

scholarly journals Gallic Acid Ameliorates Bisphenol A-Induced Toxicity in Wistar Rats

2018 ◽  
Vol 12 (4) ◽  
pp. 11-18
Author(s):  
Eunice Olufunke Ola-Davies ◽  
◽  
Samuel Gbadebo Olukole ◽  
Damilare Olaniyi Lanipekun ◽  
◽  
...  
Keyword(s):  
Bisphenol A ◽  
Gallic Acid ◽  
Adult Male ◽  
Wistar Rats ◽  
Corn Oil ◽  
Density Lipoprotein ◽  
Concomitant Treatment ◽  
Research Information ◽  
Male Wistar Rats ◽  
Liver And Kidney

Background: Bisphenol A (BPA) has received attention in environmental and toxicological research due to its widespread effects in biological systems. While several anti-oxidants have been used in ameliorating BPA-induced toxicities in experimental animals, there is the scarcity of research information on the use of Gallic acid (GA) in protecting against BPA-induced toxicity. This study investigated the ameliorative effect of Gallic acid in BPA-induced toxicities of the adult male Wistar rats. Methods: Thirty two adult male Wistar rats were randomly assigned into four groups of eight animals each as follows: Group 1 (Control rats): 0.2 ml of corn oil; Group 2 (GA-treated rats): 20 mg/kg/day GA (dissolved in distilled water); Group 3 (BPA-treated rats): 10 mg/kg/day BPA suspended in 0.2 ml corn oil; Group 4 (BPA+GA-treated rats): BPA (10 mg/kg/day) with a concomitant GA (20 mg/kg/day). All treatments were orally administered for 14 days. Results: BPA significantly increased (P<0.05) in the values of liver function enzymes (ALP, AST, ALT, GGT), total globulin, conjugated globulin, triglycerides, total cholesterol, low-density lipoprotein, creatinine, and urea as well as sodium ions. Concomitant treatment with GA ameliorated these elevated values. Moreover, BPA-induced histopathological alterations in the liver and kidney while GA ameliorated them. Conclusion: BPA caused structural and cellular perturbations of the blood, liver, and kidney of rats while concomitant treatment with GA ameliorates the condition. Hence, GA has hepato-protective and nephroprotective actions against BPA-induced toxicity in Wistar rats.

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