Grape Seed Extract Exerts Abhesive Effect Against Staphylococcus aureus: In vitro Study

2012 ◽  
Vol 7 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Marwan S.M. Al-Ni ◽  
Rana Abd-ul-Karim Rasheed ◽  
Shama Mohamed Jawad Saadaldin
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Vitro Study

scholarly journals EFFECT OF GRAPE SEED EXTRACT ON REMINERALIZATION OF ARTIFICIAL CARIES: AN IN-VITRO STUDY

2016 ◽  
Vol 9 (5) ◽  
pp. 174 ◽  
Author(s):  
Rubel M ◽  
Prashant Gm ◽  
Naveen Kumar Pg ◽  
Sushanth Vh ◽  
Mohamed Imranulla ◽  
...  
Keyword(s):  
Dental Caries ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
T Test ◽  
Vitro Study ◽  
Saline Control ◽  
Group Comparison

ABSTRACTObjective: Grape seeds are gaining more importance because the “Proanthocyanidins” present in them have excellent anticaries, remineralizing, andantibacterial properties. Although many preventive measures have been tried, dental caries remains one of the most prevalent diseases in humans.The ultimate goal of any intervention is the preservation of tooth structure, prevention of lesion progression and inducing remineralization. Thepresent in-vitro study was done to investigate the effect of grape seed extract (GSE) on artificial enamel caries in human teeth.Methods: A total of 50 sound premolars extracted from patients undergoing orthodontic treatment were used in the study. The teeth were cleanedand were immersed in a demineralizing solution for 96 hrs at 37°C and 50% relative humidity to create artificial lesions. The samples were randomlydivided into two groups of 25 each. Half of the samples were immersed in the GSE solution (test) and the other half in saline (control), respectively, for8 days. The samples were measured by microhardness tester to obtain values in Vickers hardness scale. Data were entered into Microsoft Excel andwere analyzed using independent t-test for inter-group comparison and paired t-test for intra-group comparison using SPSS version 21.Results: The mean±SD microhardness values after remineralization for the test and control groups were 357.56±5.53 and 337.60±6.38. GSE groupsignificantly increased the microhardness of the lesions (p≤0.001).Conclusion: GSE is a potential remineralizing agent and can be used to prevent progression of dental caries.Keywords: Grape seed extract, Remineralization, Dental caries.

scholarly journals The Effects of the Grape Seed Extract on Streptococcus mutans and Candida albicans: An In Vitro Study

2020 ◽  
Vol 12 (2) ◽  
pp. 35-39
Author(s):  
Masoumeh Aslanimehr ◽  
Ashraf Alizadeh ◽  
Faezeh Azmoudeh
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Large Population ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Vitis Vinifera L ◽  
Oral Pathogens

Background: Oral pathogens can affect a large population and the general health of individuals. Based on the evidence, the grape seed extract (GSE) contains herbal compounds that have the ability tosuppress oral pathogens associated with caries and fungal diseases. In this regard, the current studyaimed at evaluating the effect of GSE on Streptococcus mutans and Candida albicans. Methods: The grape (Vitis vinifera L.) seed was used in this in-vitro experimental study. After thepreparation of methanolic GSE, its effect on S. mutans and C. albicans was assessed at 0.25-256 mg/mL concentrations. Then, the minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC) were determined by macro broth dilution methods. Finally, data were analyzedby SPSS, version 20. Results: The results showed that GSE inhibits the growth of S. mutans and C. albicans. In addition, theMIC and MBC of the extract against S. mutans and C. albicans were 2 and 8 mg/mL, as well as 32 and64 mg/mL, respectively. Conclusions: In general, GSE had a significant antimicrobial effect on S. mutans, while it only affected C. albicans at high concentrations.

scholarly journals Evaluation of grape seed extract (Vitis vinifera) as a crosslinker on the stability of dentine collagen in total-etch adhesive systems: An in-vitro study

2019 ◽  
Vol 10 (4) ◽  
pp. 2811-2815
Author(s):  
Dennis D ◽  
Fenika Maitari ◽  
Trimurni Abidin ◽  
Wandania Farahanny
Keyword(s):  
In Vitro Study ◽  
Kappa Statistic ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Group Iii ◽  
Bonding Structure ◽  
Group I ◽  
The Stability

The bond strength between resin-dentine is determined by the integrity of collagen, dentine and resin monomers. The susceptibility of the dentine bond results from the instability of the bonding structure that adheres to the bonding material. This can be achieved by using collagen cross-linker, synthetically and naturally in dentine substrates which are effective in protecting collagen fibrils from degradation, as proanthocyanidine. The aim of this study was to see the effect of grape seed extract as a crosslinker on the stability of dentine collagen and see the differences between groups. This study used 27 fresh premolars or third molars which were divided into 3 groups, namely group I giving grape seed extract 6.5%, group II giving chlorhexidine 2%, and group III only giving bonding ingredients. Samples were analyzed using SEM with 1000x magnification.  Data was analyzed using the kappa statistic, Kruskal-Wallis, dan Mann-Whitney. The result of the study significant differences between treatment groups (P <0.05), the 2% chlorhexidine group showed the highest value compared to the grape seed extract group and without treatment. Grape seed extract can be used as an alternative to chlorhexidine as a crosslinker to maintain bone strength of the composite restoration

scholarly journals EFFECT OF GRAPE SEED EXTRACT SOLUTION ON THE MICROHARDNESS OF THE ROOT CANAL DENTIN: AN IN VITRO STUDY

2020 ◽  
pp. 62-65
Author(s):  
SYLVA ALINDA ◽  
ANGGRAINI MARGONO ◽  
DINI ASRIANTI
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Distilled Water ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Root Canal Dentin

Objective: Grape seed extract (GSE) containing proanthocyanidin as a root canal irrigation solution has its antibacterial effects and ability to eliminatethe smear layer. In addition, proanthocyanidin acts as a cross-linking agent of collagen, which adds to dentin’s mechanical properties. This studyanalyzed the effect of GSE containing 2.9% proanthocyanidin on the microhardness of the dentin in the root canal.Methods: Fifty teeth were divided into three groups and immersed in GSE solution, 3% NaOCl solution, or distilled water (controls) for 30 min for 3consecutive days. The microhardness was measured using the Vickers method. Data were analyzed using the Kruskal–Wallis test.Results: The GSE group presented the highest microhardness values, whereas the lowest values in the NaOCl group. No significant difference inmicrohardness observed between the GSE and distilled water groups.Conclusion: The GSE solution maintains the microhardness of the root canal dentin.

scholarly journals EVALUATION OF GRAPE SEED EXTRACT (VITIS VINIFERA) AS A CROSSLINKER ON THE STABILITY OF DENTINE COLLAGEN IN TOTAL-ETCH ADHESIVE SYSTEMS: AN IN VITRO STUDY

2019 ◽  
pp. 164-166
Author(s):  
D DENNIS ◽  
FENIKA MAITARI
Keyword(s):  
In Vitro Study ◽  
Kappa Statistic ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Group Iii ◽  
Bonding Structure ◽  
Group I ◽  
The Stability

Objective: The bond strength between resin and dentine is determined by the integrity of collagen, dentine, and resin monomers. The susceptibility of the dentine bond results from the instability of the bonding structure that adheres to the bonding material. This can be achieved using collagen cross-linker, synthetically and naturally in dentine substrates which are effective in protecting collagen fibrils from degradation, as proanthrocyanidin. The aim of this study was to see the effect of grape seed extract as a crosslinker on the stability of dentine collagen and see the differences between groups. Methods: This study used 27 fresh premolars or third molars which were divided into three Groups, namely Group I giving grape seed extract 6.5%, Group II giving chlorhexidine 2%, and Group III only giving bonding ingredients. Samples were analyzed using scanning electron microscopy with ×1000 magnification. Results: Data were analyzed using kappa statistic, Kruskal–Wallis, and Mann–Whitney. The result of the study significant differences between treatment groups (p<0.05) and the 2% chlorhexidine group showed the highest value compared to the grape seed extract group and without treatment. Conclusion: Grape seed extract can be used as an alternative to chlorhexidine as a crosslinker to maintain bone strength of the composite restoration.

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