Bioremediation of Chromium Contaminated Soil by a Brown-rot Fungus, Gloeophyllum sepiarium

2011 ◽  
Vol 6 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Varenyam Achal ◽  
Deepika Kumari ◽  
Xiangliang Pan
Keyword(s):  
Contaminated Soil ◽  
Brown Rot ◽  
Brown Rot Fungus

Bioremediation of DDT contaminated soil using brown-rot fungi

2011 ◽  
Vol 65 (5) ◽  
pp. 691-695 ◽  
Author(s):  
Adi Setyo Purnomo ◽  
Toshio Mori ◽  
Kazuhiro Takagi ◽  
Ryuichiro Kondo
Keyword(s):  
Contaminated Soil ◽  
Brown Rot ◽  
Brown Rot Fungi

scholarly journals A Lytic Polysaccharide Monooxygenase with Broad Xyloglucan Specificity from the Brown-Rot Fungus Gloeophyllum trabeum and Its Action on Cellulose-Xyloglucan Complexes

2016 ◽  
Vol 82 (22) ◽  
pp. 6557-6572 ◽  
Author(s):  
Yuka Kojima ◽  
Anikó Várnai ◽  
Takuya Ishida ◽  
Naoki Sunagawa ◽  
Dejan M. Petrovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dynamic Viscosity ◽  
Brown Rot ◽  
Primary Cell Wall ◽  
Gloeophyllum Trabeum ◽  
Lytic Polysaccharide Monooxygenase ◽  
Content Type ◽  
Brown Rot Fungus ◽  
Sensitive Method ◽  
Polysaccharide Monooxygenase

ABSTRACTFungi secrete a set of glycoside hydrolases and lytic polysaccharide monooxygenases (LPMOs) to degrade plant polysaccharides. Brown-rot fungi, such asGloeophyllum trabeum, tend to have few LPMOs, and information on these enzymes is scarce. The genome ofG. trabeumencodes four auxiliary activity 9 (AA9) LPMOs (GtLPMO9s), whose coding sequences were amplified from cDNA. Due to alternative splicing, two variants ofGtLPMO9A seem to be produced, a single-domain variant,GtLPMO9A-1, and a longer variant,GtLPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function. We have overexpressed the phylogenetically distinctGtLPMO9A-2 inPichia pastorisand investigated its properties. Standard analyses using high-performance anion-exchange chromatography–pulsed amperometric detection (HPAEC-PAD) and mass spectrometry (MS) showed thatGtLPMO9A-2 is active on cellulose, carboxymethyl cellulose, and xyloglucan. Importantly, compared to other known xyloglucan-active LPMOs,GtLPMO9A-2 has broad specificity, cleaving at any position along the β-glucan backbone of xyloglucan, regardless of substitutions. Using dynamic viscosity measurements to compare the hemicellulolytic action ofGtLPMO9A-2 to that of a well-characterized hemicellulolytic LPMO,NcLPMO9C fromNeurospora crassarevealed thatGtLPMO9A-2 is more efficient in depolymerizing xyloglucan. These measurements also revealed minor activity on glucomannan that could not be detected by the analysis of soluble products by HPAEC-PAD and MS and that was lower than the activity ofNcLPMO9C. Experiments with copolymeric substrates showed an inhibitory effect of hemicellulose coating on cellulolytic LPMO activity and did not reveal additional activities ofGtLPMO9A-2. These results provide insight into the LPMO potential ofG. trabeumand provide a novel sensitive method, a measurement of dynamic viscosity, for monitoring LPMO activity.IMPORTANCECurrently, there are only a few methods available to analyze end products of lytic polysaccharide monooxygenase (LPMO) activity, the most common ones being liquid chromatography and mass spectrometry. Here, we present an alternative and sensitive method based on measurement of dynamic viscosity for real-time continuous monitoring of LPMO activity in the presence of water-soluble hemicelluloses, such as xyloglucan. We have used both these novel and existing analytical methods to characterize a xyloglucan-active LPMO from a brown-rot fungus. This enzyme,GtLPMO9A-2, differs from previously characterized LPMOs in having broad substrate specificity, enabling almost random cleavage of the xyloglucan backbone.GtLPMO9A-2 acts preferentially on free xyloglucan, suggesting a preference for xyloglucan chains that tether cellulose fibers together. The xyloglucan-degrading potential ofGtLPMO9A-2 suggests a role in decreasing wood strength at the initial stage of brown rot through degradation of the primary cell wall.

scholarly journals Methoxy-deficient Structural Elements in Lignin of Sweetgum Decayed by a Brown-rot Fungus.

1970 ◽  
Vol 24 ◽  
pp. 3379-3390 ◽  
Author(s):  
T. Kent Kirk ◽  
Erich Adler ◽  
Olof Wahlberg ◽  
Erik Larsen ◽  
Akira Shimizu
Keyword(s):  
Brown Rot ◽  
Structural Elements ◽  
Brown Rot Fungus

Bioorganosolv pretreatments of P. radiata by a brown rot fungus (Gloephyllum trabeum) and ethanolysis

2010 ◽  
Vol 47 (1-2) ◽  
pp. 11-16 ◽  
Author(s):  
Mariel Monrroy ◽  
Jeniffer Ibañez ◽  
Victoria Melin ◽  
Jaime Baeza ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Brown Rot ◽  
Brown Rot Fungus

Micromorphological characteristics of decayed wood and laccase produced by the brown-rot fungus Coniophora puteana

2004 ◽  
Vol 50 (3) ◽  
pp. 281-284 ◽  
Author(s):  
Kwang Ho Lee ◽  
Seung Gon Wi ◽  
Adya P. Singh ◽  
Yoon Soo Kim
Keyword(s):  
Brown Rot ◽  
Decayed Wood ◽  
Coniophora Puteana ◽  
Brown Rot Fungus
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