Biodegradation of Aliphatic Hydrocarbon in Three Types of Crude Oil by Fusarium sp. F092 under Stress with Artificial Sea Water

2011 ◽  
Vol 5 (1) ◽  
pp. 64-73 ◽  
Author(s):  
A. Hidayat ◽  
S. Tachibana
Keyword(s):  
Crude Oil ◽  
Sea Water ◽  
Aliphatic Hydrocarbon ◽  
Fusarium Sp

Bioremediation of Crude Oil Contaminated Sea Water

2021 ◽  
pp. 67-79
Author(s):  
Zohra Chekroud ◽  
Mona Khamees Gouda ◽  
Moussa Houhamdi
Keyword(s):  
Crude Oil ◽  
Sea Water

Influence of Zeolites On The Degradation of Arabian Light Crude Oil

1999 ◽  
Vol 1999 (1) ◽  
pp. 1059-1063
Author(s):  
J. R. Bergueiro ◽  
A. Pita ◽  
M. A. Mayol ◽  
M. Rallo ◽  
J. López-Ruiz
Keyword(s):  
Gas Chromatography ◽  
Crude Oil ◽  
Time Evolution ◽  
Marine Bacteria ◽  
Natural Zeolite ◽  
Sea Water ◽  
Time Intervals ◽  
Light Crude Oil ◽  
Different Types ◽  
Similar Sample

ABSTRACT The authors have studied the biodegradation of an Arabian Light crude oil in sterile marine water by the CUES229 marine bacteria and the BIOLEN IG30 biologic activator, both in the presence and the absence of zeolites. Two different types of zeolites, a natural one (NZ) and an artificial one (Zestek56), were used to degrade the crude in the first experiment, arising a 20% increment in the degradation in the presence of natural zeolite when compared with a similar sample without zeolites, and a 9% in the artificial zeolite. Next set of experiment were made using reactors filled with 250 g of sea water and 0.02 g of zeolite and injecting CUES 229 bacteria up to 108 cells/ml. Samples were introduced in a shaker at 110 rpm and with regulated temperature at 30-C. Time evolution of the biodegradation was deduced from gas chromatography at the time intervals 1; 7 and 15 days. Later, a similar experiment, but with BIOLEN IG30, was also made. Biodegradation of aliphatic hydrocarbons was observed –after 15 days- until the C14 fractions, using zeolites plus Biolen IG30, and until the C18 fractions, using zeolite plus CUES 229 bacteria.

scholarly journals CYP63A2, a Catalytically Versatile Fungal P450 Monooxygenase Capable of Oxidizing Higher-Molecular-Weight Polycyclic Aromatic Hydrocarbons, Alkylphenols, and Alkanes

2013 ◽  
Vol 79 (8) ◽  
pp. 2692-2702 ◽  
Author(s):  
Khajamohiddin Syed ◽  
Aleksey Porollo ◽  
Ying Wai Lam ◽  
Paul E. Grimmett ◽  
Jagjit S. Yadav
Keyword(s):  
Molecular Weight ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Substrate Specificity ◽  
Crude Oil ◽  
Aromatic Hydrocarbons ◽  
Aliphatic Hydrocarbon ◽  
Ligand Docking ◽  
P450 Monooxygenase ◽  
Polycyclic Aromatic ◽  
Long Chain

ABSTRACTCytochrome P450 monooxygenases (P450s) are known to oxidize hydrocarbons, albeit with limited substrate specificity across classes of these compounds. Here we report a P450 monooxygenase (CYP63A2) from the model ligninolytic white rot fungusPhanerochaete chrysosporiumthat was found to possess a broad oxidizing capability toward structurally diverse hydrocarbons belonging to mutagenic/carcinogenic fused-ring higher-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs), endocrine-disrupting long-chain alkylphenols (APs), and crude oil aliphatic hydrocarbonn-alkanes. A homology-based three-dimensional (3D) model revealed the presence of an extraordinarily large active-site cavity in CYP63A2 compared to the mammalian PAH-oxidizing (CYP3A4, CYP1A2, and CYP1B1) and bacterial aliphatic-hydrocarbon-oxidizing (CYP101D and CYP102A1) P450s. This structural feature in conjunction with ligand docking simulations suggested potential versatility of the enzyme. Experimental characterization using recombinantly expressed CYP63A2 revealed its ability to oxidize HMW-PAHs of various ring sizes, including 4 rings (pyrene and fluoranthene), 5 rings [benzo(a)pyrene], and 6 rings [benzo(ghi)perylene], with the highest enzymatic activity being toward the 5-ring PAH followed by the 4-ring and 6-ring PAHs, in that order. Recombinant CYP63A2 activity yielded monohydroxylated PAH metabolites. The enzyme was found to also act as an alkane ω-hydroxylase that oxidizedn-alkanes with various chain lengths (C9to C12and C15to C19), as well as alkyl side chains (C3to C9) in alkylphenols (APs). CYP63A2 showed preferential oxidation of long-chain APs and alkanes. To our knowledge, this is the first P450 identified from any of the biological kingdoms that possesses such broad substrate specificity toward structurally diverse xenobiotics (PAHs, APs, and alkanes), making it a potent enzyme biocatalyst candidate to handle mixed pollution (e.g., crude oil spills).

scholarly journals SIMPLE SCREENING FOR POTENTIAL CHRYSENE DEGRADING FUNGI

2015 ◽  
Vol 2 (1) ◽  
pp. 364 ◽  
Author(s):  
Asep Hidayat ◽  
Sanro Tachibana
Keyword(s):  
Liquid Culture ◽  
Sea Water ◽  
Soil Samples ◽  
Malt Extract ◽  
Benzene Rings ◽  
Polycyclic Aromatic ◽  
Degradation Activity ◽  
Potential Activity ◽  
Fusarium Sp ◽  
Simple Screening

<p>Chrysene is a class of organic compounds, arranged in four benzene rings, and a polycyclic aromatic hydrocarbon (PAH), It has been found to have a variety of toxicity, mutagenicity, teratogenicity, and carcinogenicity on microorganisms, plants and animals in environment. Nowadays, the most attention on degradation of PAHs is investigating degradation of high-molecular-weight molecules. However, microbes which have ability to degrade PAHs containing more than three benzene rings are more difficult to be obtained. In order to provide chrysene degrading fungi, this study was conducted for screening, and isolating the fungi from soil, and hence investigating the selected fungi having high chrysene degradation activity. From the 62 soil samples collected from Matsuyama-Japan, 92 isolates were found and 20 isolates of them grew well in Malt extract media contaminated with chrysene (covered up 90%). Among them, a fungus, Fusarium sp. has the highest activity to degrade chrysene compared to others screened fungi. This fungus was evaluated further on liquid medium from distilled water and sea water to confirm their validity in degrading chrysene. The result showed that Fusarium sp. F092 degraded 48% of chrysene, where the chrysene degradation showed no differences at salinity of 35o/oo. The effect of variation of enzymes activities on incubation times was evaluated simultaneously. When the fungus was grown in a liquid culture, the highest activity of 1,2-dioxygenase reached 203.5 UL-1 were observed on 30 days incubation and 29.7 Ul-1 for 2,3-dioxygenase on 40 days incubation. The products of chrysene degradation by Fusarium sp. F092 are, 1-hydroxy 2-napthoic acid and catechol. In conclusion, Fusarium sp. F092 shows a high potential activity degrade PAHs contamination .</p><p><br /><strong>Keywords</strong>: Screening, chrysene, bioegradation, Fusarium sp. F092</p>

scholarly journals Isolation and characterization of hydrocarbon-degrading fungi from Ogbe-Ijoh oil creek, Warri, Delta State, Nigeria

2018 ◽  
Vol 29 (2) ◽  
pp. 72-76
Author(s):  
Faith Iguodala Akinnibosun ◽  
Abigail Ashegbare
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Ph Value ◽  
Total Carbon ◽  
Isolation And Characterization ◽  
Fungal Isolates ◽  
Hydrocarbon Content ◽  
Biodegradation Study ◽  
Fusarium Sp ◽  
Aspergillus Sp

Abstract This study was aimed at isolating and characterizing hydrocarbon-degrading fungi from hydrocarbon-contaminated soil in Ogbe-Ijoh oil creek in Warri, Delta State, Nigeria. Isolation and characterization were carried out using standard methods. Biodegradation assay was carried out over 25 days and monitored using the following parameters; pH, total organic carbon, and total hydrocarbon content. The fungi isolated were: Penicillium sp., Aspergillus niger and Fusarium sp., and these were used for the biodegradation study. Physico-chemical analysis of the crude oil contaminated soil during degradation showed that there was significant decrease in pH during degradation from day 10 - 25 (p ≥ 0.05). This showed the ability of the fungal isolates to utilize crude oil as carbon source, producing acid during its metabolism. The highest pH value during degradation by the fungal isolates was recorded in Fusarium sp. and the lowest was recorded in Aspergillus sp. The fungal isolates were able to reduce the total carbon and hydrocarbon content during degradation. This was probably due to the utilization of the nutrients in the soil sample as energy source. The results from this study indicate the potential of the isolated fungi for hydrocarbon bioremediation activity.

scholarly journals WEATHERING EXPERIMENT ON SPILLED CRUDE OILS USING A CIRCULATING WATER CHANNEL

1995 ◽  
Vol 1995 (1) ◽  
pp. 435-422 ◽  
Author(s):  
Tsutomu Tsukihara
Keyword(s):  
Crude Oil ◽  
Water Content ◽  
Sea Water ◽  
Water Channel ◽  
Crude Oils ◽  
Oil Emulsion ◽  
Circulating Water ◽  
Water In Oil Emulsion

ABSTRACT Crude oil spilled in the sea is mixed with the sea water by the wind and waves resulting in increases in its water content and viscosity as time passes. We have constructed a small, transfer type circulating water channel of an elliptical cuit-track form. Using an attached circulating unit, together with a war tunnel, artificial waves are generated to enable simulation corresponding to the natural circumstances in the sea. The experiment disclosed the following results.Drastic changes in the properties (water content and viscosity) of the oil depend on the power of waves.Contrasting processes are observed between heavy and light crude oils during weathering.Heavy crude oils form a massive water-in-oil emulsion (mousse) with increases in both water content and viscosity.Light crude oils behave differently at summer sea temperatures,

scholarly journals Rheological properties of emulsion of crude oil and water

1999 ◽  
Vol 21 (4) ◽  
pp. 213-230
Author(s):  
Duong Ngoc Hai ◽  
Nguyen Van Diep ◽  
Ha Ngoc Hien ◽  
Nguyen The Duc ◽  
Phung Dinh Thuc ◽  
...  
Keyword(s):  
Crude Oil ◽  
Rheological Properties ◽  
Sea Water ◽  
Water Concentration ◽  
Analytical Approximation ◽  
Oil Field ◽  
Wide Range ◽  
Measurement Results ◽  
Effective Dynamic ◽  
White Tiger

In the paper the rheological properties of crude oil of White Tiger oil-field (Vietnam) and its emulsion with sea-water, including measurement results and analytical approximation formulae for wide range of pressure, temperature and water concentration, are presented. As it is known, the crude oil of White Tiger oil-field is a high-paraffin and high-viscous oil. At the low temperature (T ≤ 40°C) it behaves as non-Newtonian fluid of Bingham-Shvedov group. Therefore, beside the effective viscosity, the effective dynamic shear stress is also measured and approximated. The rheological properties of crude oil and emulsion of crude oil and water are also measured and approximated for the case when the mixture contains 0.1% chemical reagent ES-3363.

scholarly journals Emulsion stability and antimicrobial activity of Ionic liquid-based formulation

2021 ◽  
Vol 287 ◽  
pp. 04003
Author(s):  
Mansoor Ul Hassan Shah ◽  
Muhammad Moniruzzaman ◽  
Mahabubur Rahman Talukder ◽  
Suzana Yusup
Keyword(s):  
Ionic Liquid ◽  
Crude Oil ◽  
Oil Spill ◽  
Sea Water ◽  
Emulsion Stability ◽  
Environmentally Benign ◽  
Oil Emulsion ◽  
Marine Oil ◽  
New Formulation ◽  
Oil Spill Remediation

Chemical dispersants is one of the globally accepted remediation technique used for marine oil spill. However, the toxicity related with these dispersants confined its application in marine environment. Therefore, to overcome this problem, the employment of environmentally benign dispersants is one of the effective conceivable approach. In this study, the formulation comprised of choline based ionic liquid, choline laurate ([Cho][Lau]) and a biosurfactant, lactonic sophorolipids (LS) were used as a crude oil emulsifier. The toxicity of the newly developed formulation was evaluated to confirm their safe employment in sea water. The developed formulation worked effectively as a crude oil emulsifier and formed a stable crude oil emulsion. The toxicity study against Gram-positive and -negative bacteria depicts the “practically harmless” nature of the developed formulation. Thus, the results presented in this study showed that the new formulation can potentially replace the conventional dispersant used for marine oil spill remediation.

scholarly journals Microaerobic conditions caused the overwhelming dominance of Acinetobacter spp. and the marginalization of Rhodococcus spp. in diesel fuel/crude oil mixture-amended enrichment cultures

2019 ◽  
Vol 202 (2) ◽  
pp. 329-342 ◽  
Author(s):  
Fruzsina Révész ◽  
Perla Abigail Figueroa-Gonzalez ◽  
Alexander J. Probst ◽  
Balázs Kriszt ◽  
Sinchan Banerjee ◽  
...  
Keyword(s):  
Crude Oil ◽  
Diesel Fuel ◽  
Enrichment Culture ◽  
Draft Genome ◽  
Aliphatic Hydrocarbon ◽  
High Abundance ◽  
Subsurface Environments ◽  
Acinetobacter Spp ◽  
Microaerobic Conditions ◽  
Shed Light

Abstract The aim of the present study was to reveal how different microbial communities evolve in diesel fuel/crude oil-contaminated environments under aerobic and microaerobic conditions. To investigate this question, aerobic and microaerobic bacterial enrichments amended with a diesel fuel/crude oil mixture were established and analysed. The representative aerobic enrichment community was dominated by Gammaproteobacteria (64.5%) with high an abundance of Betaproteobacteriales (36.5%), followed by Alphaproteobacteria (8.7%), Actinobacteria (5.6%), and Candidatus Saccharibacteria (4.5%). The most abundant alkane monooxygenase (alkB) genotypes in this enrichment could be linked to members of the genus Rhodococcus and to a novel Gammaproteobacterium, for which we generated a high-quality draft genome using genome-resolved metagenomics of the enrichment culture. Contrarily, in the microaerobic enrichment, Gammaproteobacteria (99%) overwhelmingly dominated the microbial community with a high abundance of the genera Acinetobacter (66.3%), Pseudomonas (11%) and Acidovorax (11%). Under microaerobic conditions, the vast majority of alkB gene sequences could be linked to Pseudomonas veronii. Consequently, results shed light on the fact that the excellent aliphatic hydrocarbon degrading Rhodococcus species favour clear aerobic conditions, while oxygen-limited conditions can facilitate the high abundance of Acinetobacter species in aliphatic hydrocarbon-contaminated subsurface environments.

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