scholarly journals Several Indicators of Immunity and Antioxidant Activities Improved in Grass Carp Given a Diet Containing Bacillus Additive

2012 ◽  
Vol 11 (14) ◽  
pp. 2392-2397 ◽  
Author(s):  
D.Y. Yu ◽  
W.F. Li ◽  
B. Deng ◽  
Z.W. Cui ◽  
L.Q. Fu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Antioxidant Activities

Effects of Bacillus preparations on immunity and antioxidant activities in grass carp (Ctenopharyngodon idellus)

2012 ◽  
Vol 38 (6) ◽  
pp. 1585-1592 ◽  
Author(s):  
Li Weifen ◽  
Zhang Xiaoping ◽  
Song Wenhui ◽  
Deng Bin ◽  
Liang Quan ◽  
...  
Keyword(s):  
Grass Carp ◽  
Antioxidant Activities ◽  
Ctenopharyngodon Idellus

scholarly journals Enzymatic hydrolysis of grass carp myofibrillar protein and antioxidant properties of hydrolysates

2010 ◽  
Vol 28 (No. 6) ◽  
pp. 475-484 ◽  
Author(s):  
J. Ren ◽  
H. Wang ◽  
M. Zhao ◽  
Ch. Cui ◽  
X. Hu
Keyword(s):  
Grass Carp ◽  
Incubation Temperature ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Myofibrillar Protein ◽  
Distribution Analysis ◽  
Scavenging Activity ◽  
Inhibitory Effects ◽  
Hydrolysis Of

Myofibrillar protein was extracted from grass carp, a freshwater fish, and hydrolysed using five commercial proteases (papain, pancreatin 6.0, bromelain, Neutrase 1.5MG, and Alcalase 2.4 L). The antioxidant activities of the hydrolysates were determined. Pancreatin 6.0 proved to be the most efficient protease for hydrolysing myofibrillar protein with a very high protein recovery (90.20%), its hydrolysates exhibiting the highest hydroxyl radical (&bull;OH) scavenging activity (IC<sub>50</sub> = 349.89 &plusmn; 11.50 &mu;g/ml) out of all five hydrolysates. Molecular weight distribution analysis revealed that pancreatin 6.0 hydrolysate rendered a higher proportion of the 6&minus;10 kDa fraction and a lower proportion of the 3&minus;6 kDa fraction as compared with other hydrolysates. The maximum &bull;OH scavenging activity for pancreatin 6.0 hydrolysate (IC<sub>50</sub> = 229.90 &micro;g/ml) was obtained at the enzyme to substrate ratio of 0.52%, the incubation time of 7.03 h, and the incubation temperature of 50.56&deg;C, as optimised by response surface methodology. In vitro antioxidant experiments proved that pancreatin 6.0 hydrolysates had obvious inhibitory effects on lipid peroxidation and low-density lipoproteins oxidation under optimised conditions.

Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

2020 ◽  
Vol 11 (10) ◽  
pp. 8547-8559
Author(s):  
Hongjing Zhao ◽  
Yu Wang ◽  
Mengyao Mu ◽  
Menghao Guo ◽  
Hongxian Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Human Health ◽  
Grass Carp ◽  
Aquatic Environment ◽  
Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

2017 ◽  
Vol 87 (3-4) ◽  
pp. 191-200 ◽  
Author(s):  
Nidhal Soualeh ◽  
Aliçia Stiévenard ◽  
Elie Baudelaire ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Good Alternative ◽  
Activity Levels ◽  
Salix Alba ◽  
Rosa Canina ◽  
Free Process ◽  
Plant Powders ◽  
Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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