scholarly journals Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus

2010 ◽  
Vol 9 (3) ◽  
pp. 594-600 ◽  
Author(s):  
M. Emadi ◽  
F. Jahanshiri ◽  
F. Azizi Jalalian ◽  
K. Kaveh ◽  
M.H. Bejo ◽  
...  
Keyword(s):  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain in Ethiopia 

2021 ◽  
Author(s):  
Wakjira Kebebe ◽  
Molalegne Bitew ◽  
Fufa Dawo ◽  
Bedaso Mammo ◽  
Hawa Mohammed ◽  
...  
Keyword(s):  
Vero Cell ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Vero Cells ◽  
Infectious Bursal Disease ◽  
Efficacy Evaluation ◽  
Viral Pathogen ◽  
Bursal Disease

Abstract Background: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease is endemic in Ethiopia since 2002 and vaccination is the major means of disease prevention and control. IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell; which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells, and to evaluate the immunogenicity and protection level.Results: Identity of the vaccine seed was confirmed using gene-specific primers using reverse transcription polymerase chain reaction. Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage ten. Characteristic virus induced cytopathic effect was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. Virus induced specific antibody was determined using indirect ELISA after vaccination of 14 days old chicks through ocular route. Accordingly, the antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusions: The IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibodies development and successfully protects chicks against challenging with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture with enough quantity to conquer the limitations using CEF cells and thus to vaccinate chicks to protect against IBDV infection.

scholarly journals Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

2021 ◽  
Vol Volume 12 ◽  
pp. 261-270
Author(s):  
Wakjira Kebede ◽  
Molalegne Bitew ◽  
Fufa Dawo Bari ◽  
Bedaso Mammo Edao ◽  
Hawa Mohammed ◽  
...  
Keyword(s):  
Vero Cell ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Efficacy Evaluation ◽  
Bursal Disease

scholarly journals Effect of Moringa oleifera Supplementation on Oxidative Stress Biomarkers ‎during Infectious Bursal Disease Virus Infection in Broiler Chickens

2021 ◽  
Vol 18 (3) ◽  
pp. 19-26
Author(s):  
A. G Balami ◽  
A. G. Sule ◽  
S. J. Enam ◽  
J. J. Gadzama ◽  
J. J. Ndahi ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
Infectious Bursal Disease ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Bursal Disease ◽  
Group A ◽  
Group D

This study was designed to assess the effect of Infectious Bursal Disease Virus (IBDV) on oxidative stress biomarkers in broiler chickens fed Moringa oleifera leaf (MOL) supplemented feed. Two hundred- and forty-day-old Ross 308 hybrid broiler chicks were randomly assigned into groups A, B, C and D of 60 chicks each.The chicks were raised on deep litter housing.Broiler starter (BS) and broiler finisher (BF) mash were formulated for broilers in groups A and B each with 5% MOL supplemented as part of the feed.while BS and BF were formulated without MOL for broilers in groups C and D. Broiler chickens in groups A, B and C were challenged with 0.05 ml of a live vvIBDV at 35 days of age, whereas those in group D served as controls. At days 35, 38, 42 and 49 of age, blood was collected from 10 broilers in each group via the wing vein to determine serum concentration of Catalase (CAT), Glutathione peroxidase (GPx), Superoxidase dismutase (SOD) and Malondialdehyde (MDA) using an Audiocomb Serum Auto-analyser.There was a significant increase in the concentration of CAT (P=0.0125) and GPx (P=0.0190), in broilers of group A at 38 days of age when compared with the control (group D). While the concentration of MDA increased significantly in broilers of group A (P=0.0004) at 42 days of age when compared with the control. Moringa oleifera leaf supplementation in the feed of broilers is shown to potentiate the antioxidant activities of CAT, GPx and MDA during infection with infectious bursal disease virus.

Sign in / Sign up

Export Citation Format

Share Document