scholarly journals Study on Seroprevalence of Canine Visceral Leishmaniasis (CVL) in Ownership Dogs of Sarab, East Azerbaijan, Province, Northwest of Iran with Indirect Immuno Fluorescence Antibody Test (IFAT) and its Health Importance in 2008-2009

2010 ◽  
Vol 9 (1) ◽  
pp. 139-143 ◽  
Author(s):  
M. Khanmohamm ◽  
E. Fallah ◽  
S. Rahbari ◽  
I. Sohrabi ◽  
M. Farshchian ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Northwest Of Iran ◽  
Fluorescence Antibody ◽  
East Azerbaijan

scholarly journals Occurrence of Lutzomyia longipalpis (Phlebotominae) and canine visceral leishmaniasis in a rural area of Ilha Solteira, SP, Brazil

2014 ◽  
Vol 23 (4) ◽  
pp. 456-462 ◽  
Author(s):  
Julio Cesar Pereira Spada ◽  
Diogo Tiago da Silva ◽  
Kennya Rozy Real Martins ◽  
Lílian Aparecida Colebrusco Rodas ◽  
Maria Luana Alves ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Rural Area ◽  
Antibody Test ◽  
Lutzomyia Longipalpis ◽  
Climatic Data ◽  
Canine Visceral Leishmaniasis ◽  
Serological Tests ◽  
Relative Air Humidity ◽  
Control And Prevention ◽  
One Year

This study aimed to investigate the occurrence of Lutzomyia longipalpis and also the canine visceral leishmaniasis (CVL) in a rural area of Ilha Solteira, state of São Paulo. Blood samples were collected from 32 dogs from different rural properties (small farms) and were analyzed by ELISA and the indirect immunofluorescence antibody test (IFAT) in order to diagnose CVL. From these serological tests, 31.25% of the dogs were positive for CVL and these were distributed in 66.7% (8/12) of the rural properties, which were positive for L. longipalpis. CDC (Center for Disease Control and Prevention) light traps were installed in 12 properties (one per property) and insects were caught on three consecutive days per month for one year. L. longipalpis was present on 100% of the rural properties visited, at least once during the twelve-month interval, totaling 64 males and 25 females. The insects were more numerous after the peak of the rain, but the association between prevalence of peridomestic vectors and the climatic data (precipitation, relative air humidity and temperature) and the occurrences of CVL among dogs on each rural property were not statistical significant (p <0.05). However, the occurrence of CVL cases in dogs and the presence of L. longipalpis indicate that more attention is necessairy for the control of this disease in the rural area studied.

scholarly journals Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

2010 ◽  
Vol 52 (4) ◽  
pp. 193-196 ◽  
Author(s):  
Fabiano B. Figueiredo ◽  
Maria F. Madeira ◽  
Lílian D. Nascimento ◽  
Tuanne R. Abrantes ◽  
Eliame Mouta-confort ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Filter Paper ◽  
Rio De Janeiro ◽  
Serological Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Blood Samples ◽  
One Year

The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

scholarly journals Active surveillance of canine visceral leishmaniasis and american trypanossomiasis in rural dogs from non endemic area

2011 ◽  
Vol 20 (1) ◽  
pp. 64-66 ◽  
Author(s):  
Rozeani Olimpio Tome ◽  
Fernanda Conceição Gaio ◽  
Diego Generoso ◽  
Benedito Donizete Menozzi ◽  
Helio Langoni
Keyword(s):  
Visceral Leishmaniasis ◽  
Rural Area ◽  
Vaccination Campaign ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Serological Tests ◽  
Canine Rabies ◽  
Leishmania Spp ◽  
Circulating Antibodies ◽  
Indirect Immunofluorescent

The canine visceral leishmaniasis (CVL) and american trypanosomiasis are important zoonoses in public health and dogs are the main domestic reservoir of the parasite for humans. The goal of this study was to estimate the prevalence of circulating antibodies anti-Trypanosoma cruzi and anti-Leishmania sp. in sera of dogs from the rural area of Botucatu, SP, Brazil. During the annual vaccination campaign against canine rabies in rural area, 689 blood samples were taken and processed by indirect immunofluorescent antibody test. The serological tests revealed the absence of antibodies anti-Leishmania spp., but anti-T. cruzi antibodies were detected in 3 (0.4%) dogs.

scholarly journals PARTICIPATION OF TICKS IN THE INFECTIOUS CYCLE OF CANINE VISCERAL LEISHMANIASIS, IN TERESINA, PIAUÍ, BRAZIL

2014 ◽  
Vol 56 (4) ◽  
pp. 297-300 ◽  
Author(s):  
José Henrique Furtado Campos ◽  
Francisco Assis Lima Costa
Keyword(s):  
Visceral Leishmaniasis ◽  
Bone Marrow Aspiration ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Positive Skin ◽  
Leishmania Spp ◽  
The City ◽  
Infectious Cycle ◽  
Immunofluorescence Antibody Test

In this study, we detected Leishmania spp. infection in R. sanguineus collected from dogs that were naturally infected with L. (L.) infantum. We examined 35 dogs of both sexes and unknown ages. The infected dogs were serologically positive by the immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Quick Test-DPP (Dual Path Platform), as well as parasitological examination of a positive skin biopsy or sternal bone marrow aspiration. Ten negative dogs were included as controls. The ticks that infested these dogs were collected in pools of 10 adult females per animal. The PCR was performed with specific primers for Leishmania spp., which amplified a 720-bp fragment. Of the 35 analyzed samples, a product was observed in eight samples (8/35; 22.9%). We conclude that the presence of parasite DNA suggests that ticks participate in the zoonotic cycle of canine visceral leishmaniasis, in the city of Teresina, Piauí.

scholarly journals Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis

2014 ◽  
Vol 23 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Diogo Tiago da Silva ◽  
Wilma Aparecida Starke-Buzetti ◽  
Maria Fernanda Alves-Martin ◽  
Mirian dos Santos Paixão ◽  
Michely da Silva Tenório ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Diagnostic Test ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Soluble Extract ◽  
Antigenic Protein ◽  
Complementary Method ◽  
Test Elisa ◽  
Indirect Fluorescence Antibody ◽  
Good Agreement

The aim of the present study was to evaluate the serological methods using ELISA with recombinant-rK39 (ELISA-rK-39) and soluble extract-SE (ELISA-SE) antigens, the indirect fluorescence antibody test (IFAT) in comparison to an immunochromatography rapid diagnostic test (RDT-rK39) and with a direct parasitological exam (PA) for Canine Visceral Leishmaniasis (CVL) diagnosis. The results showed that 89% (60/67) of the dogs were positive for at least one serological diagnostic test. ELISA-SE was the test that detected anti-Leishmania antibodies in the serum of the highest number of dogs (71.6%) followed by ELISA-rK39 (65.7%), IFAT (65.7%) and RDT-rK39 (55.2%). PA detected the lowest numbers (40.3%) of positive dogs. In relation to the total of examined dogs, the Kappa indexes (p ≤ 0.05) showed a good agreement between ELISA-SE and IFAT (88.1%; k = 0.7237), and it was also observed in the comparison of RDT-rK39 with ELISA-SE (83.6%, k= 0.6561), IFAT (83.5%, k= 0.6605) and PA (85.0%, k= 0.7074). A bad agreement was detected in any association of ELISA-rk39 with the other tests in either symptomatic or asymptomatic animals. ELISA as well as RDT using recombinant antigenic protein (rK39) were the methods that detected the lowest prevalence rates (33.3%) of CVL in asymptomatic dogs. In conclusion, only one test does not adequately identify dogs with CVL and it is necessary the association of two or more diagnostic tests. Because of the good agreement indexes of RDT-rK39 when evaluated with ELISA-SE, IFAT and PA it was suggested as a complementary method to be used in association with either ELISA-SE or IFAT, particularly in the symptomatic dogs. Furthermore, new studies are recommended in order to improve the sensitivity of tests mainly for asymptomatic dogs.

scholarly journals Canine visceral leishmaniasis in Barra de Guaratiba, Rio de Janeiro, Brazil: assessment of risk factors

2003 ◽  
Vol 45 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Maria Alice A. Cabrera ◽  
Adelzon A. Paula ◽  
Luis Antonio B. Camacho ◽  
Mauro Célio A. Marzochi ◽  
Samanta C. Xavier ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Rio De Janeiro ◽  
Antibody Test ◽  
Control Measures ◽  
Lutzomyia Longipalpis ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
The City ◽  
Immunofluorescent Antibody Test ◽  
Predictor Factors

Barra de Guaratiba is a coastal area of the city of Rio de Janeiro where American visceral leishmaniasis (AVL) is endemic. Although control measures including killing of dogs and use of insecticides have been applied at this locality, the canine seroprevalence remains at 25% and during 1995 and 1997 eight autochthonous human cases were notified. In order to evaluate factors related to the increase of the risk for Leishmania (Leishmania) chagasi infection in dogs we have screened 365 dogs by anti-Leishmania immunofluorescent antibody test (IFAT) and captured sandflies in the domestic and peridomestic environment. Some variables related to the infection were assessed by uni- and multivariate analysis. The distance of the residence from the forest border, its altitude and the presence of the opossum Didelphis marsupialis in the backyard, were found predictor factors for L. (L.) chagasi infection in dogs in Barra de Guaratiba. The presence of Lutzomyia longipalpis in the peridomestic environment indicates the possibility of appearence of new human cases. Our data also suggest the presence of a sylvatic enzootic cycle at this locality.

scholarly journals Canine visceral leishmaniasis: seroprevalence and risk factors in Cuiabá, Mato Grosso, Brazil

2012 ◽  
Vol 21 (4) ◽  
pp. 359-365 ◽  
Author(s):  
Arleana do Bom Parto Ferreira de Almeida ◽  
Valéria Régia Franco Sousa ◽  
Felipe Augusto Constantino Seabra da Cruz ◽  
Magyda Arabia Araji Dahroug ◽  
Fabiano Borges Figueiredo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Visceral Leishmaniasis ◽  
Univariate Analysis ◽  
Antibody Test ◽  
Urban Environments ◽  
Free Access ◽  
Canine Visceral Leishmaniasis ◽  
Mato Grosso ◽  
Rural Environments ◽  
And Control

In Brazil, canine visceral leishmaniasis (CVL) is endemic and the number of cases in humans and dogs has increased in the Midwest region. A transversal study was carried out in endemic areas from Cuiabá, State of Mato Grosso, to assess data on seroprevalence and risk factors associated to canine infection. Four hundred and thirty (430) dogs were randomly evaluated through indirect fluorescence antibody test (IFAT) considering variables related to the animals, the environment and the knowledge by owners on CVL aspects and control. From 430 dogs, 95 (22.1%) were seroreagent for leishmaniasis and animals living in rural environments present risk 1.9 times higher for acquiring the disease than those in urban environments (p = 0.01; OR 1.9). Factors related to animals' habits, such as free access to the street and guard function were considered indicators to predict infection by Leishmania sp. (p < 0.05) by statistical univariate analysis. The presence of agricultural activities was also a fact that contributed for the insurgence of the infection (p = 0.02; OR 1.68). The results contributed to the knowledge on the aspects of CVL in Cuiabá and point to an urgent need to include educational and sanitary programs in the city, since the region presents favorable characteristics for spreading the infection of CVL as already observed in other Brazilian cities.

A New Method for Detection and Localization of Urinary Infection: the Fluorescence Antibody Test

1976 ◽  
Vol 2 (3) ◽  
pp. 145-148 ◽  
Author(s):  
A. Pujol ◽  
F. Linares ◽  
J. Muñoz ◽  
A. Rusconi ◽  
V. Orejas ◽  
...  
Keyword(s):  
Urinary Infection ◽  
Antibody Test ◽  
New Method ◽  
Fluorescence Antibody ◽  
Detection And Localization

scholarly journals Amount and location of tectonic uplift in the Urmia region of northwest Iran from the Permian to the Neogene

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Akram Alizadeh
Keyword(s):  
Hypersaline Lake ◽  
Time Interval ◽  
Lake Basin ◽  
Lake Urmia ◽  
Qom Formation ◽  
Northwest Of Iran ◽  
The Subject ◽  
Orogenic Uplift ◽  
East Azerbaijan ◽  
Urmia Lake Basin

AbstractThe Urmia Lake Basin is located between the West and East Azerbaijan provinces in the northwest of Iran. Lake Urmia is the twentieth largest lake and second largest hypersaline lake in the world. Stratigraphic columns have been constructed, using published information, to compare the sedimentary units deposited from the Permian to the Neogene on the east and west sides of the lake, and to use these to quantity subsidence and uplift. East of the lake, the sedimentary section is more complete and has been the subject of detailed stratigraphic studies, including the compilation of measured sections for some units. West of the lake, the section is incomplete and less work has been done; three columns illustrate variations in the preserved stratigraphy for the time interval. In all cases, the columns are capped by the Oligocene–Miocene Qom Formation, which was deposited during a post-orogenic marine transgression and unconformably overlies units ranging from Precambrian to Cretaceous. Permian to Cretaceous stratigraphy is used to measure subsidence in the Lake Urmia basin up to the end of the Cretaceous, and then, the subsequent orogenic uplift, which was followed by further subsidence recorded by the deposition of the Qom Formation in the Oligocene–Miocene.

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