scholarly journals Confirmation of the Identity of Lactobacillus Species using Carbohydrate Fermentation Test (API 50 CHL) Identification System

2019 ◽  
Vol 19 (8) ◽  
pp. 797-802
Author(s):  
Hassan Pyar ◽  
Peh Kok
Keyword(s):  
Lactobacillus Species ◽  
Identification System ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

scholarly journals A simple carbohydrate fermentation test for identification of the pathogenic Neisseria

1975 ◽  
Vol 2 (1) ◽  
pp. 72-73
Author(s):  
A Reddick
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Mueller Hinton ◽  
Paper Disk ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
Agar Method ◽  
Disk Method ◽  
Mueller Hinton Broth ◽  
Simple Carbohydrate

The carbohydrate fermentation test in cystine-Trypticase agar-tubed medium was compared with the Minitek system with carbohydrate-impregnated paper disks in Müeller-Hinton broth for identification of Neisseria gonorrhoeae and N. meningitidis. There was 100% agreement between the methods for confirmation of N. meningitidis. The paper disk method confirmed 98% of the N. gonorrhoeae isolates; the cystine-Trypticase agar method confirmed 96%. Reactions with the paper disk method could be read in 4 h.

A new species of marine yeast Kluyveromyces penaeid isolated from the heart of penaeid shrimp Penaeus chinensis

1999 ◽  
Vol 79 (3) ◽  
pp. 559-561 ◽  
Author(s):  
Shang-Liang Tong ◽  
Hong-Zhi Miao
Keyword(s):  
New Species ◽  
Penaeid Shrimp ◽  
Heart Tissue ◽  
Urea Hydrolysis ◽  
Marine Yeast ◽  
Penaeus Chinensis ◽  
Defined Media ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
A New Species

A new species of marine yeast Kluyveromyces penaeid (Saccharomycetoideae) was isolated from the heart tissue of a subadult shrimp Penaeus chinensis during tissue culture. The yeast grew well in seawater supplemented with 2% shrimp extract, but did not grow in chemically defined media. The vegetative cells reproduced by multilateral budding and formed rudimentary pseudohyphae occasionally. Asci were spheroidal and evanescent containing 2–13 smooth or oval ascospores. The best temperature for the yeast to grow was 20–25°C and 37°C was lethal. The yeast grew well in half to full strength seawater supplemented with shrimp extract, but did not grow in 25% strength seawater. The carbohydrate fermentation test was positive, the diazonium blue B and urea hydrolysis tests were negative.

Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator

1977 ◽  
Vol 5 (1) ◽  
pp. 15-19
Author(s):  
M Slifkin ◽  
G R Pouchet
Keyword(s):  
Indicator System ◽  
Rapid Identification ◽  
Clinical Specimens ◽  
Practical Procedure ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

The Ba(OH)2 indicator system was demonstrated to be a practical procedure in assisting clinical bacteriologists in the accurate and rapid identification of the pathogenic Neisseria from clinical specimens. This system measured the release of CO2, resulting from the metabolism of fermentable carbohydrate, as the precipitated BaCO3, by means of a spectrophotometer, The method was uncomplicated and can be performed in most clinical bacteriology laboratories.

scholarly journals Carbohydrate Fermentation Test of Lactic Acid Starter Cultures

2021 ◽  
Vol 852 (1) ◽  
pp. 012035
Author(s):  
P I Gunkova ◽  
A S Buchilina ◽  
N N Maksimiuk ◽  
Y G Bazarnova ◽  
K S Girel
Keyword(s):  
Lactic Acid ◽  
Starter Cultures ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

scholarly journals The Value of The Sodium Dextro-Tartrate Fermentation Test in The Differentiation of Salmonella Organisms

1939 ◽  
Vol 39 (6) ◽  
pp. 651-657
Author(s):  
S. W. Challinor ◽  
A. J. Rhodes
Keyword(s):  
Public Health ◽  
Practical Importance ◽  
Food Poisoning ◽  
General Rule ◽  
Intestinal Infection ◽  
The Public ◽  
Health Officer ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
Paratyphoid Fever

THe identification of Salmonella cultures from cases of intestinal infection forms an important part of the routine duties of many bacteriological laboratories, and in this connexion the most important problem is to differentiate between B. paratyphosus B and the closely related food-poisoning bacilli. To the public health officer this differentiation is often a matter of great importance, for it is essential for him to know whether the case is one of paratyphoid fever or of infection by one of the food-poisoning organisms. The routine carbohydrate fermentation tests do not help to distinguish between these organisms but, as a general rule, agglutination tests are of service. Consequently, preliminary agglutination tests with “O” sera are carried out, and serve to place the organism in one of several subgroups. In this paper we are mainly concerned with organisms falling into that “0” subgroup containing B. paratyphosus B, B. aertrycke, and the “Stanley”, “Heidelberg”, “Chester”, “Derby”, “Reading”, Abortus equi and certain other strains of Salmonella (see Kauffmann, 1937). Later, tests with specific “H” sera can be performed and the cultures often accurately identified, but always the point of practical importance in such investigations is to distinguish between B. paratyphosus B and the foodpoisoning group. While it is often not of any practical importance to know the precise name of a food-poisoning bacillus, it is important to exclude the possibility of its being a strain of B. paratyphosus B.

scholarly journals Lactobacillus Species Identification, H2O2 Production, and Antibiotic Resistance and Correlation with Human Clinical Status

1999 ◽  
Vol 37 (3) ◽  
pp. 729-733 ◽  
Author(s):  
Annie Felten ◽  
Claude Barreau ◽  
Chantal Bizet ◽  
Philippe Henri Lagrange ◽  
Alain Philippon
Keyword(s):  
Clinical Status ◽  
Diffusion Method ◽  
Cell Protein ◽  
Lactobacillus Species ◽  
H2o2 Production ◽  
Hydrogen Peroxide Production ◽  
Soluble Cell ◽  
Antibiotic Susceptibilities ◽  
Carbohydrate Fermentation ◽  
Type Strains

Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility to 19 antibiotics was tested by a diffusion method, and the MICs of benzylpenicillin, amoxicillin, imipenem, erythromycin, vancomycin, gentamicin, and levofloxacin were determined. A small number of species produced H2O2, and antibiotic susceptibilities were species related. Eighteen (90%) of the isolates wereL. rhamnosus, one was L. paracasei subsp. paracasei, and one was L. crispatus. L. rhamnosus, L. paracasei subsp. paracasei isolates, and the type strains were neither H2O2 producers nor vancomycin susceptible (MICs, ≥256 μg/ml). L. crispatus, as well as most of the type strains of lactobacilli which belong to the L. acidophilus group, was an H2O2 producer and vancomycin susceptible (MICs, <4 μg/ml).

scholarly journals Simple disk-plate method for the biochemical confirmation of pathogenic Neisseria

1976 ◽  
Vol 3 (2) ◽  
pp. 172-174
Author(s):  
J A Valu
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Limited Sample ◽  
Plate Method ◽  
Simple Method ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement between the two methods when a limited sample of clinical isolates of Neisseria gonorrhoeae and Nesseria meningitidis were tested.

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