scholarly journals Genetic Relatedness Among Isolates of Acanthamoeba Based on RAPD Analysis

2005 ◽  
Vol 6 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Siti Ruhaya Abd ◽  
Shuhaimi Mustafa . ◽  
Nakisah Mat Amin . ◽  
Abdul Manaf Ali .
Keyword(s):  
Genetic Relatedness ◽  
Rapd Analysis

scholarly journals Strain Typing of Trichosporon asahii Clinical Isolates by Random Amplification of Polymorphic DNA (RAPD) Analysis

2021 ◽  
Author(s):  
Thayanidhi Premamalini ◽  
Vijayaraman Rajyoganandh ◽  
Ramaraj Vijayakumar ◽  
Hemanth Veena ◽  
Anupma Jyoti Kindo ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Strain Typing ◽  
Trichosporon Asahii ◽  
Pcr Fingerprinting ◽  
Specific Pcr ◽  
Rapd Primer ◽  
Random Amplification

Abstract Objective The aim of this study was to identify and isolate Trichosporon asahii (T. asahii) from clinical samples and to assess the genetic relatedness of the most frequently isolated strains of T. asahii using random amplification of polymorphic DNA (RAPD) primers GAC-1 and M13. Methods All the clinical samples that grew Trichosporon species, identified and confirmed by polymerase chain reaction (PCR) using Trichosporon genus-specific primers, were considered for the study. Confirmation of the species T. asahii was carried out by T. asahii-specific PCR. Fingerprinting of the most frequently isolated T. asahii isolates was carried out by RAPD using random primers GAC-1 and M13. Results Among the 72 clinical isolates of Trichosporon sp. confirmed by Trichosporon-specific PCR, 65 were found to be T. asahii as identified by T. asahii-specific PCR. Fingerprinting of the 65 isolates confirmed as T. asahii using GAC-1 RAPD primer yielded 11 different patterns, whereas that of M13 primer produced only 5 patterns. The pattern I was found to be the most predominant type (29.2%) followed by pattern III (16.9%) by GAC-1 primer. Conclusions This study being the first of its kind in India on strain typing of T. asahii isolates by adopting RAPD analysis throws light on genetic diversity among the T. asahii isolates from clinical samples. Fingerprinting by RAPD primer GAC-1 identified more heterogeneity among the T. asahii isolates than M13.

scholarly journals Genetic Characterization and Relatedness among California Almond Cultivars and Breeding Lines Detected by Randomly Amplified Polymorphic DNA (RAPD) Analysis

1998 ◽  
Vol 123 (3) ◽  
pp. 381-387 ◽  
Author(s):  
F. Bartolozzi ◽  
M.L. Warburton ◽  
S. Arulsekar ◽  
T.M. Gradziel
Keyword(s):  
Rapd Markers ◽  
Genetic Relatedness ◽  
Genetic Characterization ◽  
Rapd Analysis ◽  
Similarity Index ◽  
Prunus Dulcis ◽  
Breeding Lines ◽  
Bud Sport ◽  
Wild Almond ◽  
Variable Group

Almond [Prunus dulcis (Mill.) D.A. Webb, syn. P. amygdalus, Batsch; P. communis (I.) Archangeli] represents a morphologically and physiologically variable group of populations that evolved primarily in central and southwest Asia. California cultivars have been developed from highly selected subgroups of these populations, while new breeding lines have incorporated germplasm from wild almond and closely related peach species. The genetic relatedness among 17 almond genotypes and 1 peach genotype was estimated using 37 RAPD markers. Genetic diversity within almond was found to be limited despite its need for obligate outcrossing. Three groupings of cultivar origins could be distinguished by RAPD analysis: bud-sport mutations, progeny from interbreeding of early California genotypes, and progeny from crosses to genotypes outside the California germplasm. A similarity index based on the proportion of shared fragments showed relatively high levels of 0.75 or greater within the almond germplasm. The level of similarity between almond and the peach was 0.424 supporting the value of peach germplasm to future almond genetic improvement.

scholarly journals Genetic relatedness among Asian Cotoneaster species investigated with DNA marker analysis

2005 ◽  
Vol 11 (4) ◽  
Author(s):  
H. Nybom ◽  
I. V. Bartish ◽  
J. Fryer
Keyword(s):  
Dna Marker ◽  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Ornamental Plants ◽  
Critical Groups ◽  
Species Definition ◽  
Flora Of China ◽  
Centre Of Diversity ◽  
Principle Coordinate Analysis ◽  
Surrounding Areas

The widespread genus Cotoneaster has its centre of diversity in the Himalayas and surrounding areas. Most taxa appear to be polyploid and apomictic, and many of them have become popular ornamentals due to their attractive foliage and berries. One of the taxonomically most critical groups is Section Alpigeni which contains several important ornamental plants. The number of species belonging to this section varies widely between different taxonomic treatises, depending on whether 'splitting' or 'lumping' of species is preferred. Using a rather narrow species definition, we have investigated 13 different species using RAPD analysis. A simple matching (SM) coefficient-based principle coordinate analysis (PCO) was calculated from the RAPD data. Some species were clearly more similar to each other than to other species in the analysis. The levels of similarity did, however, not correspond very well to the lumping together of several taxa under the same species name as performed e.g. in the recent Flora of China. Obviously, the complex hybridogenous origination and, in some cases, still ongoing recombination with sexual species or among the apomictic taxa themselves, produces a genetic variability structure that cannot be properly reflected in a hierarchical taxonomy.

GENETIC RELATEDNESS AMONG OLEA EUROPAEA L. CULTIVARS ESTIMATED BY RAPD ANALYSIS

2012 ◽  
pp. 61-66 ◽  
Author(s):  
S. Gomes ◽  
P. Martins-Lopes ◽  
J. Lopes ◽  
H. Guedes-Pinto
Keyword(s):  
Olea Europaea ◽  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Olea Europaea L ◽  
Olea Europaéa

scholarly journals Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

2010 ◽  
Vol 43 (1) ◽  
Author(s):  
JULIANE R SANT’ANNA ◽  
CLÁUDIA T MIYAMOTO ◽  
LÚCIA J ROSADA ◽  
CLAUDINÉIA C S FRANCO ◽  
EDILSON N KANESHIMA ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Vegetative Compatibility ◽  
Colletotrichum Truncatum ◽  
Vegetative Compatibility Groups

scholarly journals RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey

2011 ◽  
Vol 38 (No. 4) ◽  
pp. 134-141 ◽  
Author(s):  
S. Bayazit ◽  
B. Imrak ◽  
A. Küden ◽  
M. Kemal Güngör
Keyword(s):  
Rapd Markers ◽  
Genetic Relatedness ◽  
Geographical Origin ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Cydonia Oblonga ◽  
A Minor ◽  
Almost All ◽  
Different Parts

Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions.

scholarly journals Clonal Nature of Enterotoxigenic Escherichia coliSerotype O6:H16 Revealed by Randomly Amplified Polymorphic DNA Analysis

1998 ◽  
Vol 36 (7) ◽  
pp. 2099-2102 ◽  
Author(s):  
Ana B. F. Pacheco ◽  
Kelen C. Soares ◽  
Darcy F. de Almeida ◽  
Gloria I. Viboud ◽  
Norma Binsztein ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Genetic Relatedness ◽  
Geographical Origin ◽  
Rapd Analysis ◽  
Randomly Amplified Polymorphic Dna ◽  
Significant Similarity ◽  
Single Strain ◽  
Heat Stable ◽  
Clonal Nature ◽  
Different Parts

The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

scholarly journals 090 Identification and Genetic Relatedness among Pecan Cultivars Detected by Randomly Amplified Polymorphic DNA (RAPD) Analysis

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 404B-404
Author(s):  
Patrick J. Conner ◽  
Bruce W. Wood
Keyword(s):  
Cluster Analysis ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Carya Illinoinensis ◽  
Randomly Amplified Polymorphic Dna

Genetic variation among pecan [Carya illinoinensis (Wangenh.) K. Koch] cultivars was studied using randomly amplified polymorphic DNA (RAPD) markers. Using a combination of primers, a unique fingerprint was produced for each of the pecan genotypes studied. The genetic relatedness between 44 cultivars was estimated using more than 100 RAPD markers. Genetic distances based on the simple matching coefficient varied from 0.91 to 0.59. The phenetic dendogram developed from cluster analysis showed relatively weak grouping association. However, cultivars with known pedigrees usually grouped with at least one of the parents and genetic similarity estimates appear to agree with known genetic relationships. Using RAPD information in determining genetic relationships among pecan cultivars with unknown or questionable pedigrees and the integration of that knowledge into the breeding program is discussed.

RAPD Analysis for Parentage Determination in Prunus mume Sieb. et Zucc.

1995 ◽  
Vol 64 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Takeshi Ozaki ◽  
Takehiko Shimada ◽  
Tetsu Nakanishi ◽  
Jin Yamamoto ◽  
Masao Yoshida
Keyword(s):  
Rapd Analysis ◽  
Prunus Mume
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