Detection of Serum Antibody Levels Against Infectious Bursal Disease (IBD) Virus Using Indirect Hemagglutination (IHA) Test in Commercial Broilers

2003 ◽  
Vol 2 (6) ◽  
pp. 442-445 ◽  
Author(s):  
Hussain . ◽  
I. . ◽  
M.A. Zahoor . ◽  
M.H. Rasool . ◽  
M. Shahid Mahmood . ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Infectious Bursal Disease ◽  
Indirect Hemagglutination ◽  
Bursal Disease ◽  
Antibody Levels

scholarly journals Gingyo-San Enhances Immunity and Potentiates Infectious Bursal Disease Vaccination

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Che-Ming Hung ◽  
Chia-Chou Yeh ◽  
Kowit-Yu Chong ◽  
Hsiao-Ling Chen ◽  
Jiun-Yu Chen ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Interleukin 2 ◽  
Serum Antibody ◽  
Infectious Bursal Disease ◽  
Interleukin 4 ◽  
Interleukin 12 ◽  
Cell Mediated Immunity ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Ifn Γ

The purpose of the present study was to investigate the effects of Gingyo-san (GGS), a traditional Chinese medical formula, on peripheral lymphocyte proliferation and serum antibody titers in chickens vaccinated against the infectious bursal disease (IBD) virus. Treatment groups were fed one of three doses of GGS in their diet (0.5%, 1.0% and 2.0%, w/w), and the IBD vaccine was administered at 1 and 3 weeks of age. At Weeks 8, 12 and 16, changes in serum IBD antibody titers were measured via the micro-method and T cell proliferation. In gene expression experiments, GGS-treated peripheral T lymphocytes were stimulated with concanavalin A (ConA) for 24 h. The mRNA expression of interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4 (IL-4) and interleukin-12 (IL-12) was determined using a semi-quantitative RT-PCR assay. The results showed that a low dose of GGS could significantly raise the antibody titers. Medium and high doses of GGS enhanced IL-2 and IFN-γ production. GGS altered the expression of IL-4 and IL-12 in T lymphocytes. CD4+T lymphocyte development was also skewed towards the Th1 phenotype. GGS enhanced cell-mediated immunity and augmented the effects of IBD vaccination in strengthening subsequent anti-viral responses.

scholarly journals Determination of the serum antibody level of different age groups of broiler chicken against Infectious Bursal Disease (IBD)

2016 ◽  
Vol 4 (2) ◽  
pp. 22-27
Author(s):  
MI Hossain ◽  
BK Nath ◽  
MAM Prodhan
Keyword(s):  
Antibody Titer ◽  
Broiler Chickens ◽  
Serum Antibody ◽  
Age Groups ◽  
Infectious Bursal Disease ◽  
Broiler Chicks ◽  
Protective Level ◽  
Bursal Disease ◽  
The Mean

A study on Infectious Bursal Disease (IBD) was conducted on broiler chicks (N=200+70) of strains: Cobb-500 and Hubbard classic at Laxmipur district of Bangladesh. The blood sample were collected at day old chicks (1st day), pre-vaccinated flocks (11th day) and post-vaccinated flocks (26th day), to measure the antibody titer by indirect ELISA method. Maternal derived antibody (MDA) mean titer of day old chicks (N=40) were found 9621.65 ± 780.78 and 4232.60 ± 301.66 for Cobb-500 and Hubbard classic respectively. MDA mean titer was measured at day eleven chicken (N=60) before vaccination and found 1963.00 ± 143 and 984.16 ± 126.4037 respectively. Of these two titers; Cobb-500 strain was found to be protective (>1000) where Hubbard classic was below protective (<1000) titer level. The two flocks of Cobb-500 and Hubbard classic strains of broiler chickens were vaccinated with intermediate (Bursine-2) and intermediate plus (IBD Blen) vaccine respectively at day twelve and serum was harvested on day twenty six. The mean serum titers were found 131.30 ± 36.04 and 7413.54 ± 569.39 in Cobb-500 and Hubbard classic respectively, where mean titer level of Cobb-500 was below the minimum protective level but Hubbard classic strain was developed 7413.54, protective level. These results were indicating that the maternal derived antibody titer persists above 1000 level hindering the development of antibody titer in vaccinated flocks. However, persistence of MDA titer above 1000 may causes of vaccination failure of IBD vaccine.International Journal of Natural Sciences (2014), 4(2) 22-27

The Relationship of Egg Yolk and Serum Antibody. I. Infectious Bursal Disease Virus

1989 ◽  
Vol 33 (4) ◽  
pp. 654 ◽  
Author(s):  
John Brown ◽  
R. S. Resurreccion ◽  
T. G. Dickson ◽  
Alice Horne
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Serum Antibody ◽  
Egg Yolk ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Relationship Of ◽  
The Relationship

scholarly journals Subclinical Infectious Bursal Disease in an Integrated Broiler Production Operation

1992 ◽  
Vol 4 (4) ◽  
pp. 406-411 ◽  
Author(s):  
Bruce L. Homer ◽  
Gary D. Butcher ◽  
Richard D. Miles ◽  
Alfredo F. Rossi
Keyword(s):  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Production Operation ◽  
Field Exposure ◽  
Vaccination Programs ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Lymphoid Depletion ◽  
Antibody Levels

A field study was designed to determine the prevalence of subclinical infectious bursal disease (IBD) in broiler chickens from a commercial poultry company. Bursae of Fabricius (BF) from two vaccinated and three nonvaccinated broiler flocks were evaluated histologically, and antibody profiles of these broiler and matched parent breeder flocks were established. Lesions of IBD, including lymphoid necrosis, stromal edema, and infiltrates of heterophils and macrophages, were first detected in BF at 24 days of age in both vaccinated and nonvaccinated chickens. At 41 days, all BF had lesions characteristic of IBD, including severe lymphoid depletion, proliferation of epithelial cells, and mild fibroplasia. Although mean maternal antibody levels (measured by enzyme-linked immunosorbent assay) in broilers were apparently protective through day 12, IBD antibodies decreased to nonprotective levels (below 1,000) by day 16 or 20. Titers began to increase by day 28 or 32 because of field exposure. Sentinel birds, placed with broiler flocks, also developed IBD antibody titers. Broiler breeders had low and nonuniform antibody titers. Prevalence of field IBD exposure was high, and existing vaccination programs were not effective.

scholarly journals Complete, Long-Lasting Protection against Lethal Infectious Bursal Disease Virus Challenge by a Single Vaccination with an Avian Herpesvirus Vector Expressing VP2 Antigens

2002 ◽  
Vol 76 (11) ◽  
pp. 5637-5645 ◽  
Author(s):  
K. Tsukamoto ◽  
S. Saito ◽  
S. Saeki ◽  
T. Sato ◽  
N. Tanimura ◽  
...  
Keyword(s):  
Disease Virus ◽  
Antibody Level ◽  
Infectious Bursal Disease Virus ◽  
Serum Antibody ◽  
Infectious Bursal Disease ◽  
Great Promise ◽  
Complete Protection ◽  
Cmv Promoter ◽  
Bursal Disease

ABSTRACT Marek's disease herpesvirus is a vaccine vector of great promise for chickens; however, complete protection against foreign infectious diseases has not been achieved. In this study, two herpesvirus of turkey recombinants (rHVTs) expressing large amounts of infectious bursal disease virus (IBDV) VP2 antigen under the control of a human cytomegalovirus (CMV) promoter or CMV/β-actin chimera promoter (Pec promoter) (rHVT-cmvVP2 and rHVT-pecVP2) were constructed. rHVT-pecVP2, which expressed the VP2 antigen approximately four times more than did rHVT-cmvVP2 in vitro, induced complete protection against a lethal IBDV challenge in chickens, whereas rHVT-cmvVP2 induced 58% protection. All of the chickens vaccinated with rHVT-pecVP2 had a protective level of antibodies to the VP2 antigen at the time of challenge, whereas only 42 and 67% of chickens vaccinated with rHVT-cmvVP2 or the conventional live IBDV vaccine, respectively, had the antibodies. The antibody level of chickens vaccinated with rHVT-pecVP2 increased for 16 weeks, and the peak antibody level persisted throughout the experiment. The serum antibody titer at 30 weeks of age was about 20 or 65 times higher than that of chickens vaccinated with rHVT-cmvVP2 or the conventional live vaccine, respectively. rHVT-pecVP2, isolated consistently for 30 weeks from the vaccinated chickens, expressed the VP2 antigen after cultivation, and neither nucleotide mutations nor deletion in the VP2 gene was found. These results demonstrate that the amount of VP2 antigen expressed in the HVT vector was correlated with the vaccine efficacy against lethal IBDV challenge, and complete protective immunity that is likely to persist for the life of the chickens was induced.

EVALLUTION OF TWO VACCINATION SCHEDULES FOR NEWCASTLE AND INFECTIOUS BURSAL DISEASE

2007 ◽  
Vol 6 (2) ◽  
pp. 127-132
Author(s):  
A Ali ◽  
A Nadim ◽  
A Harith ◽  
A Dina ◽  
A A.K ◽  
...  
Keyword(s):  
Infectious Bursal Disease ◽  
Bursal Disease
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