Comparative Study of Protein Profiles of the Leaves of Wild Manihot glaziovii Mueller and the Cultivated Species, Manihot esculenta Crantz by SDS-polyacrylamide Gel Electrophoresis

2005 ◽  
Vol 1 (1) ◽  
pp. 53-57 ◽  
Author(s):  
A.E. Folorunso ◽  
O.A. Awelewa . ◽  
I.O. Adewale .
Keyword(s):  
Comparative Study ◽  
Gel Electrophoresis ◽  
Manihot Esculenta ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Profiles ◽  
Manihot Esculenta Crantz ◽  
Cultivated Species ◽  
Manihot Glaziovii

A COMPARATIVE STUDY OF PREPARATIONS OF OVINE LUTEINIZING HORMONE BY BIOASSAY, IMMUNODOUBLEDIFFUSION, RADIOIMMUNOASSAY, RADIORECEPTOR ASSAY AND POLYACRYLAMIDE GEL ELECTROPHORESIS

1976 ◽  
Vol 81 (2) ◽  
pp. 270-282 ◽  
Author(s):  
M. Schlamowitz ◽  
J. Cronquist ◽  
M. Esfahani ◽  
D. N. Ward
Keyword(s):  
Luteinizing Hormone ◽  
Comparative Study ◽  
Gel Electrophoresis ◽  
Binding Capacity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Radioreceptor Assay ◽  
Polyacrylamide Gel ◽  
Polyacrylamide Gels ◽  
Biological Potency

ABSTRACT Three preparations of ovine LH were compared for biological potency and by several in vitro parameters. All were found to be heterogenous by immunodoublediffusion and by electrophoresis in polyacrylamide gels. They all also showed similarities and/or differences with respect to their characteristics in immunodoublediffusion, radioimmunoassay, radioreceptor assay, gel electrophoresis and in dye-binding capacity, but in ways that preclude establishing a meaningful correlation between biopotency and the in vitro parameters or even among the in vitro parameters themselves. The implications of these findings for the use of these in vitro parameters for screening and assessing biological potencies of LH preparations and for inferring chemical and/or structural similarities between LH preparations are discussed. Aspects of polymorphism of LH, observed by electrophoresis on polyacrylamide gels, are also discussed.

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