Adventitious Bud Induction and Plant Regeneration in Epicotyl Segments of Bakrai (Citrus reticulata BlancoxC. limetta Swing.)

2005 ◽  
Vol 1 (1) ◽  
pp. 14-19 ◽  
Author(s):  
M. Dejam . ◽  
M. Khosh-Khui . ◽  
A. Shekafandeh .
Keyword(s):  
Plant Regeneration ◽  
Adventitious Bud ◽  
Citrus Reticulata ◽  
Bud Induction ◽  
Adventitious Bud Induction

ZYGOTIC EMBRYO CULTURE OF TAXUS CHINESIS VAR. MAIREI AND PLANT REGENERATION THROUGH ORGANOGENESIS

1999 ◽  
Vol 47 (4) ◽  
pp. 287-289 ◽  
Author(s):  
Deyu Xie ◽  
Zhongchen Guo
Keyword(s):  
Plant Regeneration ◽  
Embryo Culture ◽  
Adventitious Roots ◽  
Zygotic Embryo ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Adventitious Buds ◽  
Zygotic Embryo Culture ◽  
Bud Induction ◽  
Adventitious Bud Induction

When different media were compared for their effect on zygotic embryo culture, DCR was found more effective than MS medium. From these embryos, adventitious bud induction was achieved by optimizing formular conditions as described in this paper. Adventitious buds were formed from calli derived from hypocotyls of germinated embryos. Finally, adventitious roots were induced and complete plantlets were obtained.

scholarly journals Basal stem cluster bud induction and efficient regeneration for the Tibetan endemic medicinal plant Swertia conaensis

2021 ◽  
Vol 49 (2) ◽  
pp. 12152
Author(s):  
Yin-Kai XI ◽  
Heng-Yu HUANG
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Orthogonal Experiment ◽  
Occurrence Rate ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Regeneration System ◽  
Bud Induction ◽  
Endemic Medicinal Plant

The artificial rapid propagation system for Swertia conaensis T. N. Ho et S. W. Liu was explored to screen the appropriate plant regeneration method and to provide an efficient propagation mode, useful for artificial breeding technology or for further research and development of the Tibetan endemic medicinal plant. In this study, the most suitable explant and hormone were chosen according to single factor test. Next, the effects of different hormone combinations on basal stem cluster bud induction, callus induction, adventitious bud occurrence and plant regeneration were investigated by using complete combination and orthogonal experiment. The obtained results showed that the explants suitable for in vitro of S. conaensis were stem tips with leaves, which were regenerated through the method of basal stem cluster bud occurrence in the MS medium with 2.0 mg∙L-1 6-BA, 0.5 mg∙L-1 NAA, but the proliferation coefficient was low, only 3.16 after 40 days of culture. Subsequently, the proliferation coefficient failed to improve, irrespective of change of the concentration ratio of 6-BA and NAA. Therefore, in the orthogonal experiment of adding ZT, the MS medium with 1.0 mg∙L-1 ZT, 0.5 mg∙L-1 NAA and 2.5 mg∙L-1 6-BA induced a large number of callus green and compact, with 86.30% callus occurrence rate. After 40 days of culture, the rate of adventitious bud occurrence was 96.55% and the proliferation coefficient was high (10.37). The rooting rate was 100% in the 1/2MS medium with 0.5 mg∙L-1 NAA. The survival rate of regenerated plants was more than 95%. Indirect organogenesis was more efficient than direct organogenesis in in vitro culture of S. conaensis. In this study, the efficient and stable regeneration system of S. conaensis was achieved through the method of explant to callus to adventitious buds, which provided an effective way to an endangered species.

Adventitious bud induction in Eucalyptus globulus Labill

2001 ◽  
Vol 37 (3) ◽  
pp. 388-391 ◽  
Author(s):  
Greg Nugent ◽  
Stephen F. Chandler ◽  
Phil Whiteman ◽  
Trevor W. Stevenson
Keyword(s):  
Eucalyptus Globulus ◽  
Adventitious Bud ◽  
Bud Induction ◽  
Adventitious Bud Induction
Sign in / Sign up

Export Citation Format

Share Document