Effect of Different Organic Solvents and Treatment Times on Secondary Structure and Thermal Properties of Silk Fibroin Films

2009 ◽  
Vol 2 (1) ◽  
pp. 1-9 ◽  
Author(s):  
K. Nuanchai ◽  
S. Wilaiwan ◽  
S. Prasong
Keyword(s):  
Thermal Properties ◽  
Secondary Structure ◽  
Organic Solvents ◽  
Silk Fibroin

Morphology, Secondary Structure and Thermal Properties of Silk Fibroin/Gelatin Blend Film

2009 ◽  
Vol 12 (23) ◽  
pp. 1526-1530 ◽  
Author(s):  
Ong-chiari Watcharin ◽  
Srisuwan Yaowalak ◽  
Simcheur Wilaiwan ◽  
Srihanam Prasong
Keyword(s):  
Thermal Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Blend Film

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

Redox-Active Ultrathin Template of Silk Fibroin: Effect of Secondary Structure on Gold Nanoparticle Reduction

2009 ◽  
Vol 21 (13) ◽  
pp. 2696-2704 ◽  
Author(s):  
Eugenia Kharlampieva ◽  
Dmitry Zimnitsky ◽  
Maneesh Gupta ◽  
Kathryn N. Bergman ◽  
David L. Kaplan ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Gold Nanoparticle ◽  
Silk Fibroin ◽  
Redox Active

Effect of secondary structure change on drug release from silk fibroin films

2013 ◽  
Vol 172 (1) ◽  
pp. e52 ◽  
Author(s):  
Lin Ma ◽  
Qiaona Wei ◽  
Xi Huang ◽  
Yuxiao Wang
Keyword(s):  
Secondary Structure ◽  
Drug Release ◽  
Silk Fibroin ◽  
Structure Change

A comparison of centrifugally-spun and electrospun regenerated silk fibroin nanofiber structures and properties

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98553-98558 ◽  
Author(s):  
Chen Liu ◽  
Jiaqi Sun ◽  
Min Shao ◽  
Bin Yang
Keyword(s):  
Thermal Properties ◽  
Silk Fibroin ◽  
Secondary Structures ◽  
Random Coil ◽  
Structures And Properties ◽  
Regenerated Silk Fibroin ◽  
Centrifugal Spinning ◽  
Β Sheet

Centrifugal spinning converts the conformation of silk fibroin from random coil to β-sheet more easily than electrospinning, which results in fiber differences on secondary structures, orientation and thermal properties.

scholarly journals INVESTIGATION ON THE SECONDARY STRUCTURE OF SILK FIBROIN THREAD

1980 ◽  
Vol 36 (7) ◽  
pp. T314-T316
Author(s):  
Masuhiro Tsukada ◽  
Yoshio Ishiguro ◽  
Kiyoshi Hirabayashi
Keyword(s):  
Secondary Structure ◽  
Silk Fibroin

scholarly journals Effect of pH on the Conformational Transition of Silk Fibroin in Aqueous Solution Monitored by Thioflavin-T Fluorescence

2021 ◽  
Author(s):  
Ben Jia ◽  
Lan Jia ◽  
Jingxin Zhu
Keyword(s):  
Aqueous Solution ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Conformational Transition ◽  
Thioflavin T ◽  
Random Coil ◽  
Fluorescence Dye ◽  
Assembly Behavior ◽  
Β Sheet

Abstract In this work, the potential application of the fluorescence dye Thioflavin-T (ThT), which can specifically bind to amyloid, as a powerful tool for monitoring secondary structure transitions of silk fibroin (SF) induced by pH was examined. Results showed that ThT emission intensities substantially increased when pH decreased from 6.8 to 4.8. This increase may be due to conformational transitions from random coil to β-sheet. The morphology and secondary structure of SF were also investigated via TEM, AFM and circular dichroism spectroscopy. The information obtained herein can be utilized not only for the development of convenient and efficient noninvasive method for monitoring the assembly behavior of SF in aqueous solution but also for in vitro fluorescence imaging.

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