Genetic Characterization of Myostatin and Callipyge Genes in Egyptian Small Ruminant Breeds

2015 ◽  
Vol 15 (1-2) ◽  
pp. 44-51
Author(s):  
Othman E. Othman ◽  
Esraa A. Balabel ◽  
Eman R. Mahfouz
2007 ◽  
Vol 88 (12) ◽  
pp. 3423-3427 ◽  
Author(s):  
Elena Grego ◽  
Luigi Bertolotti ◽  
Antonio Quasso ◽  
Margherita Profiti ◽  
Daniela Lacerenza ◽  
...  

In this study, characterization of the gag gene of small ruminant lentiviruses was carried out in Italian mixed flocks. The nearly complete gag gene was amplified and sequenced. Within genotype A, subtype A1 and a novel subtype, A8, were found in goats, and another novel subtype, A9, was found in both sheep and goats. Subtype B1 was found in both host species and subtype B2 was identified only in sheep. A novel, highly divergent sequence was obtained from goats in two epidemiologically related flocks and is proposed to represent a novel genotype, E. Major epitopes of matrix and capsid antigen were highly divergent, suggesting that serological identification of animals infected with genotype E may have been missed by using currently available diagnostic tests. A recombinant subunit ELISA, based on genotype E-specific epitopes, was developed and a third independent flock carrying this genotype was identified, based on serology.


2018 ◽  
Vol 65 (6) ◽  
pp. 2032-2038 ◽  
Author(s):  
Muhammad Zubair Shabbir ◽  
Aziz Ul-Rahman ◽  
Muhammad Nauman Zahid ◽  
Muhammad Munir

2013 ◽  
Vol 175 (1) ◽  
pp. 30-44 ◽  
Author(s):  
Lisa A. Santry ◽  
Jondavid de Jong ◽  
Alexander C. Gold ◽  
Scott R. Walsh ◽  
Paula I. Menzies ◽  
...  

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 183 ◽  
Author(s):  
Rodolphe Michiels ◽  
Nadjah Radia Adjadj ◽  
Nick De Regge

Small ruminant lentiviruses (SRLV) are a group of highly divergent viruses responsible for global and fatal infections in sheep and goats. Since the current phylogenetic classification of these viruses was proposed in 2004, it nowadays consists out of 5 genotypes and 28 subtypes. In support of our national SRLV control program, we performed the genetic characterization of SRLV strains circulating in the Belgian sheep and goat population. Fourteen sheep and 9 goat strains were sequenced in the gag-pol and pol regions using the method described by Shah. Most SRLV strains from sheep and goats belonged to prototype A1 and B1 subtypes, respectively. We, however, also found indications for cross-species transmission of SRLV strains between sheep and goats and vice versa, and identified a new subtype designated as B5. An in-depth analysis of the current SRLV phylogeny revealed that many subtypes have been defined over the years based on limited sequence information. To keep phylogeny as a useful tool, we advocate to apply more rigorous sequencing standards to ensure the correct classification of current and new emerging strains. The genetic characterization of Belgian SRLV strains will help in the development of appropriate diagnostic tools to assist the national control program.


2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.


Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  

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