Optimization of BH Medium for Efficient Biodegradation of Diesel, Crude Oil and Used Engine Oil by a Newly Isolated Bacillus cereus Strain DRDU1 from an Automobile Engine

2014 ◽  
Vol 13 (4) ◽  
pp. 181-185 ◽  
Author(s):  
Debajit Borah ◽  
R.N.S. Yadav
Keyword(s):  
Crude Oil ◽  
Bacillus Cereus ◽  
Engine Oil ◽  
Automobile Engine ◽  
Used Engine Oil

scholarly journals Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites

2020 ◽  
Vol 8 (12) ◽  
pp. 1912
Author(s):  
Michael Dare Asemoloye ◽  
Solveig Tosi ◽  
Chiara Daccò ◽  
Xiao Wang ◽  
Shihan Xu ◽  
...  
Keyword(s):  
Crude Oil ◽  
Aspergillus Oryzae ◽  
Hydrocarbon Degradation ◽  
Engine Oil ◽  
Broth Culture ◽  
Used Engine Oil ◽  
Saprobic Fungi ◽  
Culture Filtrates ◽  
Inhibition Response ◽  
Mucor Irregularis

Many free-living saprobic fungi are nature recruited organisms for the degradation of wastes, ranging from lignocellulose biomass to organic/inorganic chemicals, aided by their production of enzymes. In this study, fungal strains were isolated from contaminated crude-oil fields in Nigeria. The dominant fungi were selected from each site and identified as Aspergillus oryzae and Mucor irregularis based on morphological and molecular characterization, with site percentage incidences of 56.67% and 66.70%, respectively. Selected strains response/tolerance to complex hydrocarbon (used engine oil) was studied by growing them on Bushnell Haas (BH) mineral agar supplemented with the hydrocarbon at different concentrations, i.e., 5%, 10%, 15%, and 20%, with a control having dextrose. Hydrocarbon degradation potentials of these fungi were confirmed in BH broth culture filtrates pre-supplemented with 1% engine oil after 15 days of incubation using GC/MS. In addition, the presence of putative enzymes, laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) was confirmed in culture filtrates using appropriate substrates. The analyzed fungi grew in hydrocarbon supplemented medium with no other carbon source and exhibited 39.40% and 45.85% dose inhibition response (DIR) respectively at 20% hydrocarbon concentration. An enzyme activity test revealed that these two fungi produced more Lac than MnP and LiP. It was also observed through the GC/MS analyses that while A. oryzae acted on all hydrocarbon components in the used engine oil, M. irregularis only degraded the long-chain hydrocarbons and BTEX. This study confirms that A. oryzae and M. irregularis have the potential to be exploited in the bio-treatment and removal of hydrocarbons from polluted soils.

Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil

2019 ◽  
Vol 3 (5) ◽  
pp. 471-484
Author(s):  
Adeniyi Adeleye ◽  
Mohammed B. Yerima ◽  
Michael E. Nkereuwem ◽  
Victor O. Onokebhagbe ◽  
Peter G. Shiaka ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Organic Amendments ◽  
Engine Oil ◽  
Spent Engine Oil

scholarly journals Utilización de aceite quemado y paraquat en la desecación de soya y sus efectos en la calidad de la semilla durante el almacenamiento

2016 ◽  
Vol 3 ◽  
pp. 61
Author(s):  
R. Ibañez
Keyword(s):  
Environmental Conditions ◽  
Seed Quality ◽  
Rio Grande ◽  
Engine Oil ◽  
Soybean Seeds ◽  
Harvest Time ◽  
Rio Grande Do Sul ◽  
Used Engine Oil ◽  
Soybean Plants ◽  
Germination Vigor

The assay was conducted in Pelotas, Rio Grande do Sul-Brasil, to compare the effect of used engine oil with to paraquat (Gramoxone) applied during the physiological maturition of soybean seeds (Glicyne max (L.) Merrill), Bragg variety. The oil doses were 5.3, 8.5 and 12 l/ha. The desiccation intensity was determined and 30 pods per plot were periodically collected to follow the humidity content of the seeds, to 18%. The humidity was later reduced to 10.5 +0.5 and 2.0 kg of seeds were stored, in cotton bags,during 6 months under the environmental conditions at the Centro de Entrenamiento de Semillas of the Federal University of Pelotas. The seeds were subjected to germination, vigor and phytosanitary tests at harvest time and after 3 and 6 months of storage. Under the conditions of this trial, the doses of 12 l/ha of oil and 2 l/ha of paraquat: a) act as desiccants for soybean plants, b) favors the production of seeds with better viability and vigor after 6 months of storage and c) guarantied better sanitary seed quality after 6 months of storage.

Isolation of Bacillus cereus from botanical soil and subsequent biodegradation of waste engine oil

2017 ◽  
Vol 15 (7) ◽  
pp. 1453-1466 ◽  
Author(s):  
S. Bhurgri ◽  
F. N. Talpur ◽  
S. M. Nizamani ◽  
H. I. Afridi ◽  
M. A. Surhio ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Engine Oil ◽  
Waste Engine Oil
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