Isolation and Characterization of Streptomyces sp. From Soil Samples for Secondary Metabolite Production

2006 ◽  
Vol 5 (4) ◽  
pp. 478-480 ◽  
Author(s):  
K. Krishna Kumari ◽  
P. Ponmurugan . ◽  
N. Kannan .
Keyword(s):  
Secondary Metabolite ◽  
Soil Samples ◽  
Secondary Metabolite Production ◽  
Streptomyces Sp ◽  
Metabolite Production ◽  
Isolation And Characterization

scholarly journals The HosA Histone Deacetylase Regulates Aflatoxin Biosynthesis Through Direct Regulation of Aflatoxin Cluster Genes

2019 ◽  
Vol 32 (9) ◽  
pp. 1210-1228 ◽  
Author(s):  
Huahui Lan ◽  
Lianghuan Wu ◽  
Ruilin Sun ◽  
Nancy P. Keller ◽  
Kunlong Yang ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Histone Deacetylases ◽  
Trichostatin A ◽  
Secondary Metabolite Production ◽  
Fungal Development ◽  
Metabolite Production ◽  
Vitro Analysis ◽  
Aflatoxin B

Histone deacetylases (HDACs) always function as corepressors and sometimes as coactivators in the regulation of fungal development and secondary metabolite production. However, the mechanism through which HDACs play positive roles in secondary metabolite production is still unknown. Here, classical HDAC enzymes were identified and analyzed in Aspergillus flavus, a fungus that produces one of the most carcinogenic secondary metabolites, aflatoxin B1 (AFB1). Characterization of the HDACs revealed that a class I family HDAC, HosA, played crucial roles in growth, reproduction, the oxidative stress response, AFB1 biosynthesis, and pathogenicity. To a lesser extent, a class II family HDAC, HdaA, was also involved in sclerotia formation and AFB1 biosynthesis. An in vitro analysis of HosA revealed that its HDAC activity was considerably diminished at nanomolar concentrations of trichostatin A. Notably, chromatin immunoprecipitation experiments indicated that HosA bound directly to AFB1 biosynthesis cluster genes to regulate their expression. Finally, we found that a transcriptional regulator, SinA, interacts with HosA to regulate fungal development and AFB1 biosynthesis. Overall, our results reveal a novel mechanism by which classical HDACs mediate the induction of secondary metabolite genes in fungi.

scholarly journals Effective Production of Aromatic Polyketides in Streptomyces using a Combined-Culture Method

2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Shotaro Hoshino ◽  
Masahiro Okada ◽  
Hiroyasu Onaka ◽  
Ikuro Abe
Keyword(s):  
Secondary Metabolite ◽  
Spectroscopic Analysis ◽  
Culture Method ◽  
Mycolic Acid ◽  
Secondary Metabolite Production ◽  
Streptomyces Sp ◽  
Metabolite Production ◽  
Aromatic Polyketides ◽  
Fermentation Method

Combined-culture is a fermentation method which efficiently induces secondary metabolite production in Streptomyces by co-culturing them with mycolic acid-containing bacteria. As a result of combined-culture screening of our terrestrial Streptomyces collection using UV-HPLC, one of the tested strains, Streptomyces sp. TAKO-2, produced two known aromatic polyketides, julichrome Q6 (1) and julichrome Q8·8 (2), when co-cultured with the mycolic acid-containing bacterium Tsukamurella pulmonis TP-B0596. The structures of 1 and 2 were confirmed by spectroscopic analysis and literature data.

scholarly journals Discovery of Novel Glycerolated Quinazolinones from Streptomyces sp. MBT27

2018 ◽  
Author(s):  
Nataliia Machushynets ◽  
Changsheng Wu ◽  
Somayah S. Elsayed ◽  
Thomas Hankemeier ◽  
Gilles P. van Wezel
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Anthranilic Acid ◽  
Growth Conditions ◽  
Secondary Metabolite Production ◽  
Streptomyces Sp ◽  
Metabolite Production ◽  
Bioactive Natural Products ◽  
Interesting Family

AbstractActinobacteria are a major source of novel bioactive natural products. A challenge in the screening of these microorganisms lies in finding the favorable growth conditions for secondary metabolite production and dereplication of known molecules. Here, we report that Streptomyces sp. MBT27 produces 4-quinazolinone alkaloids in response to elevated levels of glycerol, whereby quinazolinones A (1) and B (2) form a new sub-class of this interesting family of natural products. Global Natural Product Social molecular networking (GNPS) resulted in a quinazolinone-related network that included anthranilic acid (3), anthranilamide (4), 4(3H)-quinazolinone (5) and 2,2-dimethyl-1,2-dihydroquinazolin-4(3H)-one (6). Actinomycins D (7) and X2 (8) were also identified in the extracts of Streptomyces sp. MBT27. The induction of quinazolinone production by glycerol combined with biosynthetic insights provide evidence that glycerol is integrated into the chemical scaffold. The unprecedented 1,4-dioxepane ring that is spiro-fused into the quinazolinone backbone, is most likely formed by intermolecular etherification of two units of glycerol. Our work underlines the importance of varying the growth conditions for the discovery of novel natural products and for understanding their biosynthesis.

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals The impact of bZIP Atf1ortholog global regulators in fungi

2021 ◽  
Author(s):  
Éva Leiter ◽  
Tamás Emri ◽  
Klaudia Pákozdi ◽  
László Hornok ◽  
István Pócsi
Keyword(s):  
Transcription Factors ◽  
Stress Response ◽  
Secondary Metabolite ◽  
Plant Pathogens ◽  
Leucine Zipper ◽  
Secondary Metabolite Production ◽  
Special Focus ◽  
Human Pathogens ◽  
Metabolite Production ◽  
The Impact

Abstract Regulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. This review highlights recent knowledge on the function of fungal orthologs of the Schizosaccharomyces pombe Atf1, Aspergillus nidulans AtfA, and Fusarium verticillioides FvAtfA, bZIP-type transcription factors with a special focus on pathogenic species. We demonstrate that fungal Atf1-AtfA-FvAtfA orthologs play an important role in vegetative growth, sexual and asexual development, stress response, secondary metabolite production, and virulence both in human pathogens, including Aspergillus fumigatus, Mucor circinelloides, Penicillium marneffei, and Cryptococcus neoformans and plant pathogens, like Fusarium ssp., Magnaporthe oryzae, Claviceps purpurea, Botrytis cinerea, and Verticillium dahliae. Key points • Atf1 orthologs play crucial role in the growth and development of fungi. • Atf1 orthologs orchestrate environmental stress response of fungi. • Secondary metabolite production and virulence are coordinated by Atf1 orthologs.

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

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