Potential Use of Random Amplified Polymorphic DNA Marker in Assessment of Genetic Diversity and Identification of Rapeseed (Brassica napus L.) Cultivars

2006 ◽  
Vol 5 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Behrouz Shiran Shiran ◽  
Roghaieh Azimkhani . ◽  
Shahram Mohammadi . ◽  
Mohammad Reza Ahmadi .
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Dna Marker ◽  
Random Amplified Polymorphic Dna ◽  
Brassica Napus L ◽  
Potential Use

Analysis of Genetic Diversity in the Brassica napus L. Gene Pool Using SSR Markers

2005 ◽  
Vol 53 (4) ◽  
pp. 793-802 ◽  
Author(s):  
M. Hasan ◽  
F. Seyis ◽  
A. G. Badani ◽  
J. Pons-Kühnemann ◽  
W. Friedt ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Ssr Markers ◽  
Gene Pool ◽  
Brassica Napus L

scholarly journals Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227990
Author(s):  
Ruikun Chen ◽  
Ayako Shimono ◽  
Mitsuko Aono ◽  
Nobuyoshi Nakajima ◽  
Ryo Ohsawa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Brassica Napus ◽  
Brassica Napus L

scholarly journals Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD

2013 ◽  
Vol 5 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Özlem ÖZBEK ◽  
Betül Uçar GIDIK
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Dna Analysis ◽  
Diversity Index ◽  
Principal Component ◽  
Brassica Napus L ◽  
Binary Matrix ◽  
Crop Species ◽  
Commercial Crop ◽  
The Mean

In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as Brassica napus L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (Hs) and total (HT) were 0.15 and 0.19 respectively. The genetic differentiation (GST) and the gene flow (Nm) values between the populations were 0.20 and 2.05 respectively. The mean number of alleles (na), the mean number of effective alleles (nae), and the mean value of genetic diversity (He) were 2.00, 1.26, and 0.19 respectively. According to Pearson’s correlation, multiple regression and principal component analyses, eco-geographical conditions in combination had significant effect on genetic indices of commercial B. napus L. varieties were discussed.

scholarly journals Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 216-225 ◽  
Author(s):  
L. Havlíčková ◽  
E. Jozová ◽  
A. Rychlá ◽  
M. Klíma ◽  
V. Kučera ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Issr Markers ◽  
Appropriate Technology ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Genetic Diversity ◽  
Diversity Assessment ◽  
Breeding Programmes

The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support  breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

scholarly journals Genetic Diversity of Chinese and Japanese Rapeseed (Brassica napus L.) Varieties Detected by RAPD Markers.

2000 ◽  
Vol 50 (4) ◽  
pp. 257-265 ◽  
Author(s):  
Chaozhi Ma ◽  
Yusuke Kimura ◽  
Hideya Fujimoto ◽  
Takako Sakai ◽  
Jun Imamura ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Rapd Markers ◽  
Brassica Napus L

scholarly journals The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1161
Author(s):  
Linh Bao Ton ◽  
Ting Xiang Neik ◽  
Jacqueline Batley
Keyword(s):  
Brassica Napus ◽  
Abiotic Stress Tolerance ◽  
Oil Quality ◽  
Canola Meal ◽  
Brassica Napus L ◽  
Oil Crops ◽  
Meal Quality ◽  
Genomic Tools ◽  
Gene Technologies ◽  
Potential Use

Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.

Analysis of Genetic Diversity in 20 Cotton Germplasm Lines Using Random Amplified Polymorphic DNA Marker

2014 ◽  
Vol 13 (4-8) ◽  
pp. 184-189 ◽  
Author(s):  
Shashank Ashokrao Tidke ◽  
S. Kiran ◽  
Sanjay Ningappa Harke
Keyword(s):  
Genetic Diversity ◽  
Dna Marker ◽  
Random Amplified Polymorphic Dna
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