scholarly journals Low Cost Material Enhanced the in vitro Regeneration and Micro Propagation of Medicinal Sand Dune Plant Species Ipomoea Pes-caprae (L.) R. Br.

2013 ◽  
Vol 9 (1) ◽  
pp. 16-23
Author(s):  
P. Thirunavuk ◽  
T. Ramanathan ◽  
G. Umamaheshw ◽  
V. Manigandan ◽  
P. Dinesh
Keyword(s):  
Plant Species ◽  
Sand Dune ◽  
In Vitro Regeneration ◽  
Low Cost ◽  
Micro Propagation ◽  
Dune Plant

scholarly journals Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species

1970 ◽  
Vol 16 (1) ◽  
pp. 19-23 ◽  
Author(s):  
S Mederos-Molina
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Plant Species ◽  
Culture Medium ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Shoots ◽  
Half Strength ◽  
Important Medicinal Plant

The purpose of this study was to establish culture medium requirements for micropropagation of Salvia broussonetii Benth., an important medicinal plant. Cultures were initiated from axillary shoots collected from mature plants. Most satisfactory results were achieved using a MS.2 medium supplemented with 1 mM ascorbic acid, 1.44 µM GA3 and 1.11 µM BAP. Axillary nodes were used for in vitro regeneration of multiple shoots and best results were achieved with MS.2 medium plus 1.44 µM GA3, 2.66 µM BAP and 1.14 µM IAA. Shoots rooted without symptoms of chlorosis or necrosis in half-strength MS.2 medium plus 1.44 µM GA3 and 2.28 µM IAA.Key words: Axillary shoots, Micropropagation, Medicinal plant, Salvia broussonetiiDOI = 10.3329/ptcb.v16i1.1101Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June)

Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis

2006 ◽  
Vol 90 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Myoung Sook Lee ◽  
Jin Ok Do ◽  
Myung Soo Park ◽  
Sera Jung ◽  
Kang Hyun Lee ◽  
...  
Keyword(s):  
Plant Species ◽  
Sand Dune ◽  
Coastal Sand Dune ◽  
Coastal Sand ◽  
Dune Plant

scholarly journals PLANT TISSUE CULTURE: A TOOL TO CONSERVE BIODIVERSITY THROUGH RAPID IN VITRO REGENERATION OF DIVERSE PLANT SPECIES

2017 ◽  
pp. 132-139
Author(s):  
Nirali Vora
Keyword(s):  
Tissue Culture ◽  
Plant Species ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Ornamental Plants ◽  
Cost Effective ◽  
Human Consumption ◽  
Natural Forests

Biodiversity is declining with the loss of natural forests across the world. Plant tissue culture is an important biotechnological tool to raise large number of plant species in short spa of time. However, commercial tissue culture laboratories are working on raising plantlets important for human consumption only; which mainly include fruit crops, ornamental plants, timber-yielding forest trees and medicinal plants. There is an urgent need of raising all the different plant species rapidly through tissue culture. Through cultivation of these high yielding and disease-free crops in the forests for consumption of all other species of fauna; conservation of biodiversity can be achieved. However, as tissue culture plantlets are costlier than conventionally raised plants, despite of its advantages its utility is limited. To reduce cost of an important fruit crop, banana during its in vitro regeneration, cost-effective alternatives are proposed.

scholarly journals Erratum to: Dominance of Lysobacter sp. in the rhizosphere of two coastal sand dune plant species, Calystegia soldanella and Elymus mollis

2012 ◽  
Vol 103 (2) ◽  
pp. 443-447
Author(s):  
Myoung Sook Lee ◽  
Jin Ok Do ◽  
Myung Soo Park ◽  
Sera Jung ◽  
Kang Hyun Lee ◽  
...  
Keyword(s):  
Plant Species ◽  
Sand Dune ◽  
Coastal Sand Dune ◽  
Coastal Sand ◽  
Dune Plant

scholarly journals Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation

2016 ◽  
Vol 4 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Resham Babu Amgai ◽  
Hari Kumar Prasai ◽  
Yama Raj Pandey
Keyword(s):  
Tissue Culture ◽  
In Vitro Regeneration ◽  
Flower Bud ◽  
Shoot Bud ◽  
Hormonal Effect ◽  
Medium Level ◽  
Micro Propagation ◽  
Planting Materials ◽  
Mandarin Orange

Tissue culture is the best option to produce disease free seedling of the fruit crop rapidly. Micro-propagation and use of the in-vitro grafting (micro-grafting) is very helpful for production of virus free planting materials in mandarin. Different levels of the in-vitro hormone affect the success of callusing, shooting and plant regeneration in mandarin. Shoot bud, flower bud and in-vitro seedling epicotyl was used as explants to study the hormonal effect on mandarin micro-propagation. Similarly, 10 levels of BAP and IAA combination on MS media for mandarin tissue culture were used. Observation was done for 100 test tubes per treatment combination after 4, 8 and 12 weeks of culture. Data was arc sine transformed for analysis. Shooting from explants was significantly higher (71.72%) on medium level of the BAP (0.5 mg/L) and IAA (0.2 mg/L) using in-vitro seedling stem as explant, however, it was 27.91% for stem bud as explant. Stem bud showed higher level of callusing (6.15%, p<0.001) in mandarin orange. However, flower bud didn’t develop shoot in mandarin tissue culture. Increment of the in-vitro regeneration of the shooting and callusing was observed by the increment of the in-vitro incubation duration in mandarin orange tissue culture.

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

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