An Improved Plant Regeneration System for High Frequency Multiplication of Rubia cordifolia L.: A Rare Medicinal Plant

2011 ◽  
Vol 3 (4) ◽  
pp. 397-405 ◽  
Author(s):  
Swaroopa Ghatge ◽  
Subhash Kudale ◽  
Ghansham Dixit
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
High Frequency ◽  
Regeneration System ◽  
Frequency Multiplication ◽  
Rubia Cordifolia

scholarly journals Basal stem cluster bud induction and efficient regeneration for the Tibetan endemic medicinal plant Swertia conaensis

2021 ◽  
Vol 49 (2) ◽  
pp. 12152
Author(s):  
Yin-Kai XI ◽  
Heng-Yu HUANG
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Orthogonal Experiment ◽  
Occurrence Rate ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Regeneration System ◽  
Bud Induction ◽  
Endemic Medicinal Plant

The artificial rapid propagation system for Swertia conaensis T. N. Ho et S. W. Liu was explored to screen the appropriate plant regeneration method and to provide an efficient propagation mode, useful for artificial breeding technology or for further research and development of the Tibetan endemic medicinal plant. In this study, the most suitable explant and hormone were chosen according to single factor test. Next, the effects of different hormone combinations on basal stem cluster bud induction, callus induction, adventitious bud occurrence and plant regeneration were investigated by using complete combination and orthogonal experiment. The obtained results showed that the explants suitable for in vitro of S. conaensis were stem tips with leaves, which were regenerated through the method of basal stem cluster bud occurrence in the MS medium with 2.0 mg∙L-1 6-BA, 0.5 mg∙L-1 NAA, but the proliferation coefficient was low, only 3.16 after 40 days of culture. Subsequently, the proliferation coefficient failed to improve, irrespective of change of the concentration ratio of 6-BA and NAA. Therefore, in the orthogonal experiment of adding ZT, the MS medium with 1.0 mg∙L-1 ZT, 0.5 mg∙L-1 NAA and 2.5 mg∙L-1 6-BA induced a large number of callus green and compact, with 86.30% callus occurrence rate. After 40 days of culture, the rate of adventitious bud occurrence was 96.55% and the proliferation coefficient was high (10.37). The rooting rate was 100% in the 1/2MS medium with 0.5 mg∙L-1 NAA. The survival rate of regenerated plants was more than 95%. Indirect organogenesis was more efficient than direct organogenesis in in vitro culture of S. conaensis. In this study, the efficient and stable regeneration system of S. conaensis was achieved through the method of explant to callus to adventitious buds, which provided an effective way to an endangered species.

scholarly journals Establishment of high frequency plant regeneration system from leaf explants of Pinellia koreana via bulblets formation

2009 ◽  
Vol 36 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Myung-Jin Oh ◽  
Jong-Mi Park ◽  
Bu-Youn Lee ◽  
Pil-Son Choi ◽  
Kyoung-Hwan Tae ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Leaf Explants ◽  
Regeneration System

High-frequency plant regeneration and histological analysis of callus in Cichorium intybus: An important medicinal plant

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
K. Dakshayini ◽  
C. Vaman Rao ◽  
Anitha Karun ◽  
U. Bhavyashree ◽  
P. Ujwal
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Medicinal Plant ◽  
High Frequency ◽  
Leaf Disc ◽  
Leaf Explants ◽  
Cichorium Intybus ◽  
Naphthalene Acetic Acid ◽  
Regenerated Plants

<p>An efficient in vitro propagation and in vitro flowering protocols were developed for the medicinal plant Cichorium intybus (Asteraceae) using leaf disc explants. Media supplemented with the growth regulator naphthalene acetic acid (NAA) (1.5 mg/l) + 6-benzyle adenine (0.25 mg/l) was used for the initial induction of the callus and further subcultured to the same media for the proliferation of the callus. Pale yellow and green calli were noticed, which depends on incorporation of the growth hormones and their varying concentrations. Murashige and Skoog medium in addition with 2 mg/l kinetin+ 0.5 mg/l indole-3-acetic acid (IBA) + 500 mg/l casein hydrolysate resulted in maximum regeneration. Media supplemented via IBA (0.5 mg/l) and NAA (0.5 mg/l) (98%) was found to be<br />optimum for rhizogenesis for in vitro regenerated plants. For acclimatization 5-6 weeks mature in vitro regenerated plants were transferred into the greenhouse for acclimatization. The histological study revealed the presence actively dividing meristematic cells in callus. The occurrence of the peripheral meristematic zone associated with callus was noticed in after 20 days, which formed the shoot meristems after 45 days of incubation. To our knowledge, this is the first report on high-frequency plant regeneration which was carried out indirectly from the<br />leaf explants which was grown in controlled environment with varying concentration of the growth regulators and histology of callus of different stages from leaf explants of C. intybus.</p>

Sign in / Sign up

Export Citation Format

Share Document