Some Biochemical Effects of Sub-Acute Oral Administration of L-Arginine on Monosodium Glutamate-Fed Wistar Albino Rats 2: Serum Alkaline Phosphatase, Total Acid Phosphatase and Aspartate Aminotransferase Activities

2010 ◽  
Vol 5 (2) ◽  
pp. 89-95 ◽  
Author(s):  
A.C.C. Egbuonu ◽  
C.A. Ezeokonkwo ◽  
P.M. Ejikeme ◽  
O. Obidoa ◽  
L.U.S. Ezeanyika
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Oral Administration ◽  
Aspartate Aminotransferase ◽  
Serum Alkaline Phosphatase ◽  
Monosodium Glutamate ◽  
Albino Rats ◽  
Total Acid ◽  
Biochemical Effects ◽  
Wistar Albino Rats

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Intraperitoneal Administration ◽  
Chloroform Extract ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
High Dose ◽  
Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

scholarly journals BIOCHEMICAL EVALUATION OF CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

2021 ◽  
pp. 99-102
Author(s):  
SURENDRA BABU THANGACHI ◽  
VARSHA SRIRAM MOKHASI ◽  
SHABINA KOMATH CHENOLY
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Total Bilirubin ◽  
Liver Enzymes ◽  
Monosodium Glutamate ◽  
Density Lipoprotein ◽  
Lipid Profiles ◽  
Albino Rats ◽  
High Dose ◽  
Wistar Albino Rats

Objective: The objective of this study was to determine if there were any harmful effects of monosodium glutamate (MSG) on the liver of Wistar albino rats chronically at three different doses, namely, low, mid, and high doses equivalent to human consumption doses in developing countries. Methods: The Wistar albino rats (n=24) were divided into four groups, namely control, Low dose MSG (180 mg/kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). At the end of the experimental period (120 days), animal blood was collected retro-orbitally to analyze the liver enzymes such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Total protein, Albumin, and Total Bilirubin in blood serum. Lipid profiles, namely, Triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and Total cholesterol were subjected to analysis using blood serum. Results: Significant increase (p<0.05) in AST, ALT, ALP, and total bilirubin in serum of MSG induced low, mid, and high dose groups when compared to control group were recorded. There was a significant increase (p<0.05) in LDL, decrease in HDL, increase in total cholesterol and triglycerides of MSG-induced animal groups. Conclusion: The effects of MSG on serum liver enzymes and lipid profiles in this present animal study were not severely alarming even though the dosage was chronic which opens further discussion on the controversies revolving around MSG.

Fluorometric Assay of Serum Acid or Alkaline Phosphatase, Either in Solution or on a Semisolid Surface

1975 ◽  
Vol 21 (12) ◽  
pp. 1791-1794 ◽  
Author(s):  
Bernd Rietz ◽  
George G Guilbault
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Clinical Laboratory ◽  
Serum Alkaline Phosphatase ◽  
Buffer Solution ◽  
Citrate Buffer ◽  
Enzymatic Action ◽  
Stable Substrate ◽  
Serum Acid Phosphatase

Abstract We describe enzymatic fluorometric methods for determining activities of serum alkaline phosphatase and of serum acid phosphatase in solution and on silicone rubber pads. 4-Methylumbelliferone phosphate is used as substrate, in either tris(hydroxymethyl)aminomethane or citrate buffer. In solution, the reaction is measured at 37 °C in a 3-ml Pyrex cuvette. Measurements on the pads are also made at 37 °C, after establishing a stable substrate film by lyophilizing all reagents on the surface of the pads. Only 20 to 30 µl of substrate solution, 50 µl of buffer solution, and 1 to 10 µl of blood are necessary, making a total volume of 51 to 60 µl for each assay. The rate of appearance of the fluorescent 4-methylumbelliferone liberated from 4-methylumbelliferone phosphate by the enzymatic action is measured and equated to enzyme activity. Calibration plots of the change in fluorescence per minute vs. enzyme activity for measurements in solution and on pads show a good proportionality in the range of 30.8 to 633 U/liter for alkaline phosphatase and in the range of 0.265 to 5.3 King— Armstrong units for acid phosphatase, indicating the usefulness of these methods in the clinical laboratory.

scholarly journals A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Henning Staedt ◽  
Michael Dau ◽  
Eik Schiegnitz ◽  
Daniel G. E. Thiem ◽  
Olga Tagadiuc ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Turnover ◽  
Bone Substitute ◽  
Bone Alkaline Phosphatase ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Total Acid ◽  
Turnover Markers

Abstract Background The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n = 20) and without (“-”;n = 20) collagen membrane was applied. Blood and bone was collected 24, 72 h, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points. Results Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-” (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p < 0.05). Fourteen days after surgery, the significant highest values for all bone turnover markers were detected in “-” (all p < 0.05). A significant difference in favor of group “-” could also be detected after 3 weeks in terms of both acid phosphatases (p < 0.05). In histology, no significant differences could be detected. Conclusion Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

Low dose oral administration of monosodium glutamate in male albino rats may be nephroprotective

Bio-Research ◽  
2009 ◽  
Vol 7 (1) ◽  
Author(s):  
ACC Egbuonu ◽  
O Obidoa ◽  
CA Ezeokonkwo ◽  
LU Ezeanyika ◽  
PM Ejikeme
Keyword(s):  
Oral Administration ◽  
Low Dose ◽  
Monosodium Glutamate ◽  
Albino Rats ◽  
Dose Oral

Evaluation of Toxicity and Antidiabetic Activity of Ethanolic Extract of Flowers of Moringa Oleifera Against Dexamethasone Induced Hyperglycemia in Albino WistarRats

2020 ◽  
Vol 2 (29) ◽  
pp. 72-83
Author(s):  
Sourabh Jain Neha Jain
Keyword(s):  
Insulin Resistance ◽  
Oral Administration ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Serum Glucose ◽  
The Body ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Antidiabetic Effect ◽  
Wistar Albino Rats

Abstract-Diabetes is a defect in the ability of the body to convert glucose (sugar) to energy. Glucose is the main source of energy in our body. When food is digested it is metabolized into fats, proteins, or carbohydrates. Glucose is then transferred to the blood and is used by the cells for energy production. To investigate the antidiabetic effect ethanolic extracts of flowers of Moringa oleifera against dexamethasone induced insulin resistance in wistar albino rats. To study the antidiabetic effect, flowers ofMoringaoleiferawerecollectedandauthenticated, extracted and investigated for acute toxicity and dexamethasone induced hyperglcemia. The animals treated with EEMOF at a dose of 100mg/kg and 200mg/kg prevented the development ofhyperglycemia,hypercholesteremiaandhypertriglyceridemia in dexamethasone induced insulin resistance models. Oral administration of Moringa Oleifera 100mg/kg and 200mg/kg reduces serum glucose, triglyceride, total cholesterols and LDLconcentration and improve the concentration of HDLin dexamethasone administered rats. The lignin Moringa Oleifera showed significant anti-diabetic effect in rats after oral administration. The present study demonstrated that Moringa Oleifera could be useful in Management of diabetes associated with abnormalities in lipid profiles. Further study need to isolate, identify the active compounds and find out thepossiblemechanismofactions.

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