Effects of High Temperature Stress on mRNA Levels of Ubiquitin-activating Enzyme in Ovarian Tissue of Goldfish, Carassius auratus

2012 ◽  
Vol 7 (4) ◽  
pp. 356-360
Author(s):  
A. Bayir ◽  
M.F. Polat ◽  
N.M. Aras ◽  
B. Nalbantogl
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
Carassius Auratus ◽  
Ovarian Tissue ◽  
High Temperature Stress ◽  
Mrna Levels ◽  
Goldfish Carassius Auratus

scholarly journals Reference Gene Selection for Quantitative Real-Time PCR of Mycelia fromLentinula edodesunder High-Temperature Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Xu Zhao ◽  
Huanling Yang ◽  
Mingjie Chen ◽  
Xiaoxia Song ◽  
Changxia Yu ◽  
...  
Keyword(s):  
High Temperature ◽  
Real Time ◽  
Temperature Stress ◽  
Reference Gene ◽  
Reference Genes ◽  
Candidate Reference Gene ◽  
Housekeeping Genes ◽  
High Temperature Stress ◽  
Stable Gene ◽  
Mrna Levels

Housekeeping genes are important for measuring the transcription expression of functional genes; 10 traditional reference genes,TUB, TUA, GADPH, EF1, 18S, GTP, ACT, UBI, UBC,andH2A, were tested for their adequacy inLentinula edodes(L. edodes). Using specific primers, mRNA levels of these candidate housekeeping genes were evaluated in mycelia ofL. edodes, which were treated with high-temperature stress at 37°C for 0, 4, 8, 12, 18, and 24 hours. After treatment, expression stability of candidate genes was evaluated using three statistical software programs: geNorm, NormFinder, and BestKeeper. According to geNorm,TUBhad the lowest M values inL. edodesstrains 18 and 18N44. Using NormFinder, the best candidate reference gene in strain 18 wasTUB(0.030), and the best candidate reference gene in strain 18N44 wasUBI(0.047). In BestKeeper analysis, the standard deviation (SD) values ofUBC,TUA,H2A,EF1,ACT,18S, andGTPin strain 18 and those ofGADPHandGTPin strain 18N44 were greater than 1; thus, these genes were disqualified as reference genes. Taken together, onlyUBIandTUBwere found to be desirable reference genes by BestKeeper software. Based on the results of three software analyses,TUBwas the most stable gene under all conditions and was verified as an appropriate reference gene for quantitative real-time polymerase chain reaction inL. edodesmycelia under high-temperature stress.

THE ROLE OF THE PITUITARY IN THE RESISTANCE OF THE GOLDFISH (CARASSIUS AURATUS L.) TO A HIGH TEMPERATURE

1967 ◽  
Vol 45 (3) ◽  
pp. 329-345 ◽  
Author(s):  
Peter H. Johansen
Keyword(s):  
High Temperature ◽  
Heat Resistance ◽  
Carassius Auratus ◽  
Essential Role ◽  
High Temperature Stress ◽  
Heat Resistant ◽  
Goldfish Carassius Auratus ◽  
Normal Fish ◽  
Resistance Tests

The heat resistance of normal, hypophysectomized and pituitary-autotransplanted goldfish acclimated to 20 °C under 16-h photoperiod and 8-h photoperiod conditions was determined. It was found that hypophysectomized goldfish were less heat resistant than normal fish and that those with an autotransplanted pituitary were as heat resistant as normal fish.The photoperiod results, though inconclusive, suggest that normal 16-h photoperiod fish are more heat resistant than normal 8-h photoperiod fish and that only the fish with a normal pituitary can respond to photoperiod changes. The fish without a pituitary are slightly less heat resistant than normal 8-h-photoperiod fish, while those possessing an autotransplanted pituitary are as resistant to a high temperature as normal 16-h-photo period fish.Treatment of hypophysectomized goldfish with luteinizing, thyrotropic, adrenocorticotropic, and lactotropic (prolactin) hormones and crude salmon pituitary extract appeared not to affect resistance to a high temperature.In the experiments involving pituitary autotransplantation, a study was made of various organs influenced by pituitary secretions to determine the pattern of hormone output by the pituitary. By contrasting this information with that of the heat resistance tests, various hormones have been suggested as not playing an essential role in high temperature resistance. The hypothesis is put forward that a prolactin-like hormone plays the essential role in resisting a high-temperature stress.

Assessment of wheat (Triticum aestivum L.) genotypes for high temperature stress tolerance using physico-chemical analysis

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Khalil Ahmed Laghari ◽  
Abdul Jabbar Pirzada ◽  
Mahboob Ali Sial ◽  
Muhammad Athar Khan ◽  
Jamal Uddin Mangi
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Chemical Analysis ◽  
Stress Tolerance ◽  
Temperature Stress ◽  
Triticum Aestivum L ◽  
High Temperature Stress ◽  
Physico Chemical

Genome-wide identification and analysis of maize PAL gene family and its expression profile in response to high-temperature stress

2020 ◽  
Vol 52 (5) ◽  
Author(s):  
De-Gong Wu ◽  
Qiu-Wen Zhan ◽  
Hai-Bing Yu ◽  
Bao-Hong Huang ◽  
Xin-Xin Cheng ◽  
...  
Keyword(s):  
High Temperature ◽  
Gene Family ◽  
Temperature Stress ◽  
Expression Profile ◽  
High Temperature Stress ◽  
Genome Wide

Impact of Metal Pad Etch-Induced Plasma Damage on Dynamic Retention Time Degradation during High Temperature Stress in High Density DRAM Technology

2005 ◽  
Author(s):  
D-J Kim ◽  
I-G Kim ◽  
J-Y Noh ◽  
H-J Lee ◽  
S-H Park ◽  
...  
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
Retention Time ◽  
High Temperature Stress ◽  
High Density ◽  
Cell Junction ◽  
Antenna Effect ◽  
Plasma Damage ◽  
Root Cause ◽  
Wafer Processing

Abstract As DRAM technology extends into 12-inch diameter wafer processing, plasma-induced wafer charging is a serious problem in DRAM volume manufacture. There are currently no comprehensive reports on the potential impact of plasma damage on high density DRAM reliability. In this paper, the possible effects of floating potential at the source/drain junction of cell transistor during high-field charge injection are reported, and regarded as high-priority issues to further understand charging damage during the metal pad etching. The degradation of block edge dynamic retention time during high temperature stress, not consistent with typical reliability degradation model, is analyzed. Additionally, in order to meet the satisfactory reliability level in volume manufacture of high density DRAM technology, the paper provides the guidelines with respect to plasma damage. Unlike conventional model as gate antenna effect, the cell junction damage by the exposure of dummy BL pad to plasma, was revealed as root cause.

Effect of high temperature stress on seed filling and nutritional quality of rice (Oryza sativa L.)

2020 ◽  
Vol 16 (2) ◽  
pp. 18-23
Author(s):  
K. PRAVALLIKA ◽  
C. ARUNKUMAR ◽  
A. VIJAYKUMAR ◽  
R. BEENA ◽  
V. G. JAYALEKSHMI
Keyword(s):  
Oryza Sativa ◽  
High Temperature ◽  
Temperature Stress ◽  
Nutritional Quality ◽  
High Temperature Stress ◽  
Seed Filling

scholarly journals Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 687
Author(s):  
Chan Seop Ko ◽  
Jin-Baek Kim ◽  
Min Jeong Hong ◽  
Yong Weon Seo
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Two Dimensional Gel Electrophoresis ◽  
Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

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