Effect of PR toxin on THP1 and Caco-2 cells: an in vitro study

2017 ◽  
Vol 10 (4) ◽  
pp. 375-386 ◽  
Author(s):  
N. Hymery ◽  
O. Puel ◽  
S. Tadrist ◽  
C. Canlet ◽  
H. Le Scouarnec ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Penicillium Roqueforti ◽  
Fold Reduction ◽  
Cytotoxicity Studies ◽  
Food And Feed ◽  
Gene Expression Studies ◽  
Toxin Degradation ◽  
Factor Α

Penicillium roqueforti produces mycotoxins including PR toxin, which is a food and feed contaminant. In this study, PR toxin was purified from culture material of the Penicillium roqueforti F43-1 strain. Toxic effects were evaluated in undifferentiated human Caco-2 intestinal epithelial cells and THP-1 monocytic immune cells. To understand the mechanisms involved in PR-toxin toxicity, cell death and pro-inflammatory gene expression were studied. In addition, PR toxin degradation was assessed. Cytotoxicity studies showed a dose-dependent effect of PR toxin and the calculated mean cytotoxic concentration (IC50) concentrations were for Caco-2 and THP-1 cells >12.5 and 0.83 μM, respectively. Gene expression studies showed that tumour necrosis factor-α expression was significantly increased after 24 h exposure to 312 μM PR toxin. PR toxin induced necrosis on THP-1 cells after 3 h exposure. In the cell culture system, the PR toxin showed a 10-fold reduction in PR toxin concentration within 48 h, indicating that PR toxin was degraded by THP-1. To conclude, PR toxin appears to be one of the most cytotoxic P. roqueforti mycotoxins on Caco-2 and/or THP-1 cells and induces in THP-1 cells both necrosis and an inflammatory response.

An in-vitro study investigating the effect of air-abrasion bioactive glasses on dental adhesion, cytotoxicity and odontogenic gene expression

2021 ◽  
Author(s):  
Gianrico Spagnuolo ◽  
Paula Maciel Pires ◽  
Anna Calarco ◽  
Gianfranco Peluso ◽  
Avijit Banerjee ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Vitro Study ◽  
Air Abrasion ◽  
Bioactive Glasses

Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants

Lab on a Chip ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Olaf Wagner ◽  
Julian Thiele ◽  
Marie Weinhart ◽  
Linas Mazutis ◽  
David A. Weitz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Encapsulation ◽  
Droplet Microfluidics ◽  
Side Chain ◽  
Expression Studies ◽  
Chain Composition ◽  
Gene Expression Studies

Polyglycerol-based triblock surfactants with tailored side-chain composition are exemplified in cell encapsulation and in vitro gene expression studies in droplet-based microfluidics as alternative to PEG-based surfactants.

scholarly journals Effects of Berries on High-Fat Diet-Induced Inflammation

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 449-449
Author(s):  
Patricia Perez ◽  
Desiree Wanders ◽  
Hannah Land ◽  
Kathryn Chiang ◽  
Rami Najjar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Inflammatory Markers ◽  
In Vitro Study ◽  
Vitro Study ◽  
In Vivo Study ◽  
High Fat ◽  
Anti Inflammatory

Abstract Objectives Studies suggest that inflammation mediates the link between obesity and its comorbidities including type 2 diabetes and cardiovascular disease. Hence, there is a demand for effective alternative or complementary approaches to treat obesity-associated inflammation. The objective of this study was to determine whether consumption of blackberries (BL) and raspberries (RB) alone or in combination reduce obesity-induced inflammation. Methods In Vitro Study: RAW 264.7 macrophages were pretreated with either BL, RB, or BL + RB, each at a final concentration of 200 µg/mL for 2 h. LPS (1 ng/mL) was then added to the media for 16 h. mRNA expression of inflammatory cytokines was measured. In Vivo Study: Five-week-old mice were acclimated to a low-fat low-sucrose (LFLS) diet for one week after which mice were randomized 10 per group to one of five groups: 1) LFLS, 2) high-fat high-sucrose (HFHS), 3) HFHS + 10% BL, 4) HFHS + 10% RB, or 5) HFHS + 5% BL + 5% RB. Expression of inflammatory markers was measured in the liver as well as epididymal and inguinal white adipose tissue. Results In Vitro Study: Each berry alone and in combination suppressed the LPS-induced increase in inflammatory markers, with the combination (BL + RB) having the greatest effect. The combination suppressed LPS-induced expression of Ccl2, Tnfa, F4/80, and Il6 by 3.7−, 5.3−, 5.3−, and 4.4-fold, respectively. In Vivo Study: Gene expression analysis indicated that berry consumption had no significant effect on proinflammatory (Ccl2, Il1b, Tnfa, Il6, Itgam) or anti-inflammatory (Adipoq, Arg1, Mgl1) markers in adipose tissue depots or liver. However, relatively low gene expression of inflammatory markers in the tissues indicates that the mice fed the HFHS diet failed to develop a robust inflammatory state. Conclusions BL and RB have direct anti-inflammatory effects on immune cells. Initial analysis indicates that consumption of BL and RB has no significant effects on markers of inflammation in a diet-induced mouse model of obesity. However, it is possible that the relatively low levels of inflammation in these mice masked the anti-inflammatory potential of BL and RB. Ongoing analysis will provide additional insights into the effects of BL and RB on inflammation in these tissues. Funding Sources Lewis Foundation Award.

Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model

2014 ◽  
Vol 46 (3) ◽  
pp. 206-212 ◽  
Author(s):  
M. Goswami ◽  
B.S. Sharma ◽  
Kamalendra Yadav ◽  
S.N. Bahuguna ◽  
W.S. Lakra
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
In Vitro Model ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Vitro Model

Effect of leptin and photoperiod on pituitary SOCS-3 gene expression in sheep – in vitro study

2013 ◽  
Vol 13 ◽  
pp. 15-16
Author(s):  
Małgorzata Szczęsna ◽  
Katarzyna Kirsz ◽  
Mirosław Kucharski
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Vitro Study
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