Mycotoxins content and its association with changing patterns of Fusarium pathogens in wheat in the Czech Republic

2017 ◽  
Vol 10 (2) ◽  
pp. 143-151 ◽  
Author(s):  
T. Sumíková ◽  
J. Chrpová ◽  
Z. Džuman ◽  
J. Salava ◽  
L. Štěrbová ◽  
...  

Mycotoxin content in 244 samples of wheat ears randomly collected during 2014 and 2015 from various localities in the Czech Republic was analysed using liquid chromatography coupled to mass spectrometry (LC-MS). Mean mycotoxin concentration in 2014 was highest for deoxynivalenol (DON; 760 μg/kg), followed by zearalenone (ZEA; 115 μg/kg), 3-acetyldeoxynivalenol (3-ADON; 88 μg/kg), deoxynivalenol-3-glucoside (83 μg/kg), and enniatins (ENNs; 102 μg/kg). In 2015, DON (66 μg/kg) also had the highest concentration level, followed by ENNs (35 μg/kg), nivalenol (2 μg/kg), and beauvericin (2 μg/kg). The maximum limit for DON in the European Union (1,250 μg/kg) was exceeded in 2% of samples, and the maximum limit for ZEA (100 μg/kg) was exceeded in 0.8% of samples. Fusarium species causing head blight were identified using PCR assays. During 2014-2015, Fusarium poae considerably dominated (48.7% average value of occurrence in the samples). Other species were detected in much lower frequencies in both years: Fusarium graminearum (average frequency of occurrence 13.7%), Fusarium avenaceum (11.9%), Fusarium culmorum (4.2%), and Fusarium equiseti (2.9%). Fusarium langsethiae was identified only in 2015, at a frequency of 10.2%, and Fusarium sporotrichioides was present only sporadically in 2014.

2016 ◽  
Vol 9 (2) ◽  
pp. 317-327 ◽  
Author(s):  
J. Chrpová ◽  
V. Šíp ◽  
T. Sumíková ◽  
J. Salava ◽  
J. Palicová ◽  
...  

Random collections of ears of winter wheat cultivars grown within the Czech Republic were made during 2004-2013. The ears were assessed for Fusarium head blight symptoms on a 0-9 scale and grain samples were analysed for deoxynivalenol (DON) content by enzyme-linked immunosorbent assay. Samples from 2011-2013 were analysed in greater detail, including to detect Fusarium species using polymerase chain reaction assays and determine mycotoxin content (including ‘emerging’ mycotoxins) using ultra-high performance liquid chromatography coupled with tandem mass spectrometry. The 10-year survey reveals a significant adverse effect from maize as the preceding crop on the accumulation of DON. Most endangered were those areas situated in the eastern part of the country. The study revealed prevailing occurrence of Fusarium poae and Fusarium graminearum within the Czech Republic. The occurrence of F. poae increased greatly in 2012 (with 93.3% of samples infected) in association with relatively warmer and drier weather. These investigations provide abundant evidence as to the presence of so-called ‘emerging’ mycotoxins, particularly enniatins. The survey also shows that increased content of ‘emerging’ mycotoxins could be accompanied by high DON content through mixed infections. Among examined toxins, only DON and DON-3-glucoside (DON-3G) were positively correlated (r=0.800; P<0.001) while levels of the remaining toxins were independent of one another. DON production by both DON-producing species F. graminearum and Fusarium culmorum was significantly higher in mixed infections with other species. The presence of Fusarium avenaceum with other species appeared markedly to promote the production of 5 out of 6 mycotoxins, namely DON, DON-3G, nivalenol, enniatins, and beauvericin. These five mycotoxins profited also from contamination by F. poae. Moreover, the presence of F. graminearum and F. culmorum appeared to be decisive for zearalenone accumulation, which was not correlated with DON levels.


2021 ◽  
pp. 1-12
Author(s):  
I. Polišenská ◽  
O. Jirsa ◽  
J. Salava ◽  
I. Sedláčková ◽  
J. Frydrych

Deoxynivalenol and zearalenone content and the presence of Fusarium species in organically and conventionally grown wheat harvested in the Czech Republic during 2015-2017 were studied. Mycotoxin contamination was compared using two approaches. The first was based on samples taken from randomly selected farms in individual regions of the Czech Republic, both organic (154 samples) and conventional (330 samples). In the second approach, a sample set of conventional wheat compatible with organic one was formed, with samples paired according to the preceding crop and region of harvest. Using the first approach, mycotoxins were shown to be higher in conventional wheat; however, there was no difference in mycotoxin contamination between organic and conventional wheat using the second approach. Eight wheat samples with mycotoxin content above the EU limits were found, seven for deoxynivalenol (2.1%) and one for zearalenone (0.3%), all of them originated from conventional farming system. Six of them had maize as the preceding crop. The presence of five Fusarium species (Fusarium avenaceum, Fusarium culmorum, Fusarium graminearum, Fusarium poae and Fusarium sporotrichioides) was compared for the compatible wheat sample sets (second approach). The predominant species were found to be F. poae and F. graminearum in both the organic and conventional wheat. Harvest year significantly influenced both the occurrence of the main Fusarium species and the level of mycotoxin contamination. The study confirmed that (a) organic farming system was able to keep mycotoxin contamination of wheat at a low level, (b) in studies on the significance of organic/conventional agricultural practice on mycotoxin levels in cereals, it is important to pay attention to common production and environmental variables.


Author(s):  
Michal Onderco

This chapter focuses on defence transformations in the Czech Republic, Slovakia, and Hungary since the end of the cold war. The three lesser powers of Central Europe all eventually joined NATO and the European Union, following the fall of the Iron Curtain. The process they underwent completely transformed their security strategies and military doctrines, but the plans to transform their military forces have developed slowly, and the actual process has been interrupted and incomplete. This chapter addresses the development of civil–military relations, the main milestones in the development of the respective states’ national security policies, and the main changes in the structure of military forces in each of these countries. Finally, the chapter looks at the nascent trends towards military cooperation between the three countries, including military sharing and joint procurement.


2020 ◽  
Vol 10 (10) ◽  
pp. 3647
Author(s):  
Peter Fiener ◽  
Tomáš Dostál ◽  
Josef Krása ◽  
Elmar Schmaltz ◽  
Peter Strauss ◽  
...  

In the European Union, soil erosion is identified as one of the main environmental threats, addressed with a variety of rules and regulations for soil and water conservation. The by far most often officially used tool to determine soil erosion is the Universal Soil Loss Equation (USLE) and its regional adaptions. The aim of this study is to use three different regional USLE-based approaches in three different test catchments in the Czech Republic, Germany, and Austria to determine differences in model results and compare these with the revised USLE-base European soil erosion map. The different regional model adaptations and implementation techniques result in substantial differences in test catchment specific mean erosion (up to 75% difference). Much more pronounced differences were modelled for individual fields. The comparison of the region-specific USLE approaches with the revised USLE-base European erosion map underlines the problems and limitations of harmonization procedures. The EU map limits the range of modelled erosion and overall shows a substantially lower mean erosion compared to all region-specific approaches. In general, the results indicate that even if many EU countries use USLE technology as basis for soil conservation planning, a truly consistent method does not exist, and more efforts are needed to homogenize the different methods without losing the USLE-specific knowledge developed in the different regions over the last decades.


2012 ◽  
Vol 8 (1) ◽  
pp. 1-7 ◽  
Author(s):  
LB ◽  
JHR

In between the writing of this editorial and the publication of this issue of EuConst, the Treaty on Stability, Coordination and Governance in the Economic and Monetary Union, in everyday parlance the ‘Fiscal Compact’, will have been signed by the representatives of the governments of the contracting parties — the member states of the European Union minus the United Kingdom and the Czech Republic. The Fiscal Compact is intended to foster budgetary discipline, to strengthen the coordination of economic policies and to improve the governance of the euro area.


Author(s):  
Sebastian Kubala ◽  
Marcin Stanuch

One of the key markets of the European Union is the poultry meat market. The situation on this market is of significant importance to both producers and consumers. The main aim of the article is to study the level of self-sufficiency of selected countries in Central and Eastern Europe in the production of poultry meat. Eleven countries were selected as the research area: Bulgaria, Croatia, the Czech Republic, Estonia, Lithuania, Latvia, Poland, Romania, Slovakia, Slovenia and Hungary. In the conducted research, simplified indices of self-sufficiency were used: the balance of foreign trade in terms of volume and value, average annual consumption per capita and production volume in relation to consumption. The study was conducted for the years 2009-2018. The statistical sources used come from FAOSTAT and International Trade Centre databases. Research has shown that the analyzed countries are characterized by a significant diversification of the self-sufficiency level of poultry meat production. Only 6 analyzed countries can meet the domestic consumption demand from their own production, the remaining countries supplement the deficit from imported goods. The highest level of self-sufficiency of poultry meat production was observed in Poland, Slovenia, Lithuania and Hungary. On the other hand, the lowest level is found in Bulgaria, the Czech Republic, Estonia, Slovakia and Latvia.


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