Synthetic routes to isotopologues of acetylated derivatives of deoxynivalenol to be used in stable isotope dilution assays

2012 ◽  
Vol 5 (3) ◽  
pp. 297-302 ◽  
Author(s):  
S. Asam ◽  
M. Rychlik
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Stable Isotope Dilution ◽  
Advantages And Disadvantages ◽  
Contaminated Food ◽  
Food And Feed ◽  
Acetyl Group ◽  
Stable Isotope Dilution Assays ◽  
Synthetic Routes ◽  
Derivatives Of

The isomers 3-O-acetyl- and 15-O-acetyldeoxynivalenol (3-ADON and 15-ADON) are intermediates of deoxynivalenol (DON) biosynthesis. Both compounds are present along with DON in contaminated food and feed, but they are not analysed routinely. This review describes synthetic routes to stable isotope labelled 3-ADON and 15-ADON that can be used as internal standards in stable isotope dilution assays. The label was introduced either as [2H3]-acetyl or [13C2]-acetyl group in all protocols. Regioselective acetylation can be obtained by the use of protection groups or stepwise acetylation and hydrolysis. Advantages and disadvantages of both strategies are discussed.

scholarly journals Folate bioavailability from foods rich in folates assessed in a short term human study using stable isotope dilution assays

2015 ◽  
Vol 6 (1) ◽  
pp. 241-247 ◽  
Author(s):  
Sabine Mönch ◽  
Michael Netzel ◽  
Gabriele Netzel ◽  
Undine Ott ◽  
Thomas Frank ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Human Study ◽  
Short Term ◽  
Standard Definition ◽  
Stable Isotope Dilution ◽  
Stable Isotope Dilution Assays ◽  
Folate Bioavailability ◽  
Definition Of ◽  
Different Sources

Different sources of folate may have different bioavailability and hence may impact the standard definition of folate equivalents.

scholarly journals Characterization of the Key Aroma Constituents in Fry Breads by Means of the Sensomics Concept

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1129
Author(s):  
Ola Lasekan ◽  
Fatma Dabaj
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Aroma Extract Dilution Analysis ◽  
Butanoic Acid ◽  
Active Constituents ◽  
Stable Isotope Dilution ◽  
Stable Isotope Dilution Assays ◽  
Baked Potato ◽  
Phenyl Acetaldehyde

The key aroma constituents in the volatile fractions isolated FROM two differently processed fry breads by solvent-assisted flavor evaporation were characterized by an aroma extract dilution analysis (AEDA). Twenty-two compounds were identified with flavor dilution (FD) factor ranges of 2–516. Among them, 13 compounds (FD ≥ 16) were quantified by stable isotope dilution assays and analyzed by odor activity values (OAVs). Of these, 11 compounds had OAVs ≥ 1, and the highest concentrations were determined for δ-decalactone and 2,3-butanedione. Two recombination models of the fry breads showed similarity to the corresponding fry breads. Omission tests confirmed that aroma-active constituents, such as δ-decalactone (oily/peach), 2-acetyl-1-pyrroline (roasty/popcorn-like), 3-methylbutanal (malty), methional (baked potato-like), 2,3-butanedione (buttery), phenyl acetaldehyde (flowery), (E,E)-2,4-decadienal (deep-fried), butanoic acid, and 3-methylbutanoic acid, were the key aroma constituents of fry bread. In addition, 3-methoxy-4-vinylphenol (smoky) and 4-hydroxy-2,5-dimethyl-3(2H)-furanone were also identified as important aroma constituents of fry bread.

scholarly journals Quantitation of 5-methyltetraydrofolic acid in plasma for determination of folate status and clinical studies by stable isotope dilution assays

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0212255
Author(s):  
Lisa Striegel ◽  
Beate Brandl ◽  
Markus Kopp ◽  
Lukas Sam ◽  
Thomas Skurk ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Clinical Studies ◽  
Folate Status ◽  
Stable Isotope Dilution ◽  
Stable Isotope Dilution Assays
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