Saccharomyces cerevisiae strains from animal environment with in vitro aflatoxin B1 binding ability and anti-pathogenic bacterial influence

2011 ◽  
Vol 4 (1) ◽  
pp. 59-68 ◽  
Author(s):  
M. Armando ◽  
C. Dogi ◽  
R. Pizzolitto ◽  
F. Escobar ◽  
M. Peirano ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Vero Cells ◽  
Molecular Techniques ◽  
Production Environment ◽  
Binding Ability ◽  
Yeast Strains ◽  
Gastrointestinal Conditions

Mycotoxins are metabolites produced by fungi growing on foods or feeds and represent a serious hazard to humans and animals. Concerns related to the negative health impact of aflatoxins have led to the investigation of strategies to prevent, eliminate or reduce the presence of these toxins in contaminated products. Saccharomyces cerevisiae strains are among promising candidates that can be used in animal feed for improving the robustness of animals in the production environment. The aim of this work was to isolate and select S. cerevisiae strains from pig environment with aflatoxin B1 (AFB1) binding ability, able to tolerate gastrointestinal conditions and with some potential beneficial properties to the host. S. cerevisiae strains were isolated from animal feed, faeces and gut and identified by morphological and molecular techniques. AFB1 binding percentages varied among yeast strains according to the AFB1 concentration used. The RC016 strain showed the highest adsorption percentage at the three AFB1 concentrations tested in this work (50, 100 and 500 ng/ml) followed by RC008 strain. All yeast strains were able to survive under gastrointestinal conditions and to strongly adhere to Vero cells. All S. cerevisiae strains showed co-aggregation with pathogenic bacteria (Escherichia coli, Enterobacter cloacae and Salmonella enterica sub sp. enterica). Only RC016 and RC008 strongly inhibited the three pathogens assayed. S. cerevisiae strains RC016 and RC008 are promising microorganisms for inclusion in animal feed.

scholarly journals Saccharomyces cerevisiae as a probiotic agent and a possible aflatoxin B1 adsorbent in simulated fish intestinal tract conditions

2020 ◽  
Vol 72 (3) ◽  
pp. 862-870
Author(s):  
R.E.E. Pinheiro ◽  
A.M.D. Rodrigues ◽  
C.E. Lima ◽  
J.T.O. Santos ◽  
C.M. Pereyra ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aflatoxin B1 ◽  
Pathogenic Bacteria ◽  
Intestinal Tract ◽  
Fish Farming ◽  
In Vitro Tests ◽  
Yeast Strains ◽  
Gastrointestinal Conditions ◽  
Self Aggregation

ABSTRACT The aim of this study was to evaluate in vitro the probiotic potential and absorption of Saccharomyces cerevisiae for the aflatoxin B1 in simulated fish intestinal tract conditions. Three yeast strains were used, two from brewery: S. cerevisiae RC1 and S. cerevisiae RC3 and one from a fish farming environment: S. cerevisiae A8L2. The selected yeasts were subjected to the following in vitro tests: homologous inhibition, self-aggregation, co-aggregation, antibacterial activity, gastrointestinal conditions tolerance and adsorption of AFB1. All S. cerevisiae strains showed good capability of self-aggregation and co-aggregation with pathogenic bacteria. All yeast strains were able to survive the gastrointestinal conditions. In acidic conditions, the factors (strain vs. time) had interaction (P=0.0317), resulting in significant variation among the strains tested in the time periods analyzed. It was observed that there was also interaction (P=0.0062) in intestinal conditions, with an increased number of cells in the 12-hour period for all strains tested. In the adsorption test, the A8L2 strain was statistically more effective (P<0.005) for both AFB1 concentrations evaluated in this study (10 and 25ng/mL). Thus, it was observed that the strains of S. cerevisiae have potential probiotic and adsorbent of AFB1.

scholarly journals Potential of Inulin-Fructooligosaccharides Extract Produced from Red Onion (Allium cepa var. viviparum (Metz) Mansf.) as an Alternative Prebiotic Product

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2401
Author(s):  
Jakkrit Aisara ◽  
Pairote Wongputtisin ◽  
Somkid Deejing ◽  
Chutamas Maneewong ◽  
Kridsada Unban ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Probiotic Bacterium ◽  
Degree Of Polymerization ◽  
Inhibitory Effects ◽  
Commercial Cultivation ◽  
Gastrointestinal Conditions ◽  
Free Glucose ◽  
Probiotic Lactobacilli ◽  
Bifidogenic Effect

Red onion is a popular ingredient in many Thai dishes and has recently been promoted for commercial cultivation. In this study, inulin-fructooligosaccharides (inulin-FOSs) were extracted from red onions in a simplified extraction method. The extract contained 24.00 ± 0.38 g/L free glucose, fructose and sucrose, while the level of FOSs was recorded at 74.0 ± 2.80 g/L with a degree of polymerization of 4.1. The extract was resistant to simulated gastrointestinal conditions, while selectively promoting probiotic lactobacilli. These outcomes resulted in inhibitory effects against various pathogenic bacteria. The in vitro batch culture fermentation of the extract by natural mixed culture indicated that an unknown sugar identified as neokestose was more rapidly fermented than 1-kestose and other longer-chain inulin-FOSs. Notably, neokestose selectively encouraged a bifidogenic effect, specifically in terms of the growth of Bifidobacteirum breve, which is an infant-type probiotic bacterium. This is the first report to state that neokestose could selectively enhance the bifidogenic effect. In summary, inulin-FOSs extract should be recognized as a multifunctional ingredient that can offer benefits in food and pharmaceutical applications.

Binding ability of phthalate monoesters by Saccharomyces cerevisiae strains in vitro

Food Control ◽  
2019 ◽  
Vol 102 ◽  
pp. 11-16
Author(s):  
Yuan-ting Zhu ◽  
Jing-hui Lai ◽  
Xu-duo Liao ◽  
Ze-ren Ge-rong ◽  
Shu-liang Liu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Binding Ability

scholarly journals Potential probiotic yeast isolated from an Indonesian indigenous fermented fish (Ikan Budu)

10.5219/1544 ◽  
2021 ◽  
Vol 15 ◽  
pp. 460-466
Author(s):  
Yetti Marlida ◽  
Nurul Huda ◽  
Harnentis ◽  
Yuliaty Shafan Nur ◽  
Nuri Mekar Lestari ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Bile Acid ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Acid Tolerance ◽  
Fermented Food ◽  
Probiotic Properties

Budu is a fermented food resulting from the activities of microorganisms like lactic acid bacteria and yeast. Budu, therefore, serves as a source of probiotics that can have beneficial effects on livestock and humans. Nonetheless, their selection has to be done with caution. The current study purposed to find out whether budu has desirable probiotic properties. This was done by determining its pH, bile acid tolerance, hydrophobicity, and inhibition of pathogens such as Staphylococcus aureus, Salmonella enteritidis, and Escherichia coli. An in vitro experiment was conducted using three Saccharomyces cerevisiae (coded as SC 11, SC 12, and SC 21) in the preparation of budu. The whole experiment was repeated four times. The budus were tested for their probiotic properties (low pH, bile salts, hydrophobicity, and inhibition of pathogenic bacteria). The results showed that the three Saccharomyces cerevisiae survived in gastric juice and bile acid, exhibited good hydrophobicity, and could inhibit pathogenic bacteria, both gram-positive and negative pathogens. They were able to survive at pH 2 for 3 h (40.70 to 55.1%), at pH 2 for 5 h (35.25 to 46.88%), in 0.3% bile acid incubated for 3 h (69.69 to 86.56%), in 0.3% bile acid incubated for 5 h (82.22 to 88.18%) and hydrophobicity ability of 97.0 to 98.1%. The inhibition activity against pathogenic bacteria, that is, Escherichia coli was 2.50 to 3.81 mm, Staphylococcus aureus was 1.66 to 3.71 mm, and Salmonella enteritidis was 1.20 to 2.64 mm.

scholarly journals Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
C. Sumathi ◽  
D. MohanaPriya ◽  
S. Swarnalatha ◽  
M. G. Dinesh ◽  
G. Sekaran
Keyword(s):  
Secondary Metabolite ◽  
Pathogenic Bacteria ◽  
Vero Cells ◽  
Esi Ms ◽  
Fish Gut Bacteria ◽  
Fish Gut ◽  
Tannery Solid Waste ◽  
Optimization Of Fermentation ◽  
Cancerous Cells

Aim. The focal theme of present investigation includes isolation of prodigiosin producing fish gut bacteria, enhancing its production using tannery solid waste fleshing, and evaluation of its pharmacological effect.Methods. Optimization of fermentation conditions to yield maximum prodigiosin, and instrumental analysis using FTIR, NMR, ESI-MS, TGA, and DSC.Results. The optimum conditions required for the maximum prodigiosin concentration were achieved at time 30 h, temperature 30°C, pH 8, and 3% substrate concentration. The secondary metabolite was analyzed using ESI-MS, FTIR, and NMR. Therapeutic efficacy was assessed by in vitro anticancer studies. Among the pathogenic bacteriaPseudomonas aeruginosawas most susceptible at the lowest concentration followed bySalmonellatyphi. IC50concentration was cell line specific (HeLa cells: 4.3 µM, HEp2: 5.2 µM, and KB cells: 4.8 µM) and remains nontoxic up to the concentration of 25 µM on normal Vero cells suggesting that cancerous cells are more susceptible to the prodigiosin at lower concentration.Conclusion. Maximum prodigiosin production was obtained with tannery fleshing. The potency of the fish gut bacterial secondary metabolite prodigiosin as a therapeutic agent was confirmed through in vitro antimicrobial and anticancer studies.

scholarly journals Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

2021 ◽  
Vol 22 (18) ◽  
pp. 10004
Author(s):  
Rubén Agregán-Pérez ◽  
Elisa Alonso-González ◽  
Juan Carlos Mejuto ◽  
Nelson Pérez-Guerra
Keyword(s):  
Animal Feed ◽  
Low Cost ◽  
Skim Milk ◽  
Acetic Acid Bacteria ◽  
Probiotic Properties ◽  
The European Union ◽  
Gastrointestinal Conditions ◽  
Probiotic Product ◽  
Kefir Grains

Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation of whey with a combination of kefir grains, AGK1, and the fermented whole milk used to activate these kefir grains. The probiotic culture obtained was characterized by high levels of biomass (8.03 g/L), total viability (3.6 × 108 CFU/mL) and antibacterial activity (28.26 Activity Units/mL). Some probiotic properties of the probiotic culture were investigated in vitro, including its survival at low pH values, under simulated gastrointestinal conditions, after freezing in skim milk at −20 °C, and in the commercial feed during storage at room temperature. The viable cells of lactic and acetic acid bacteria and yeasts exhibited higher tolerance to acidic pH and simulated gastrointestinal conditions when the cells were protected with skim milk and piglet feed, compared with washed cells. The results indicated the feasibility of producing a probiotic product at a low cost with a potential application in animal feed.

scholarly journals IN VITRO ASSESSMENT OF POTENTIAL PROBIOTIC MICROORGANISMS FOR APPLICATION IN ANIMAL FEEDING

2018 ◽  
Vol 54 (4A) ◽  
pp. 250 ◽  
Author(s):  
Le Thi Hong Van
Keyword(s):  
Enzyme Production ◽  
Animal Feed ◽  
Digestive Enzyme ◽  
Saccharomyces Boulardii ◽  
Bacillus Sp ◽  
Acid Generation ◽  
Gastrointestinal Conditions ◽  
Animal Feeding ◽  
In Vitro Assessment

This study aimed to evaluate and to select potential probiotic microorganisms. The obtained results would be further studied for application in production of animal feed. A total of 16 strains of microorganisms including 11 strains of Lactobacillus, four strains of Bacillus and a yeast strain Saccharomyces boulardii PLCP were investigated for acid production, digestive enzyme production and antimicrobial activity as well as their survival when exposed to simulated gastrointestinal conditions. The results showed that all 11 strains of Lactobacillus bacteria were capable of acid generation (in the range from 18.05 – 19.04 g/l). All four strains of Bacillus bacteria were capable of producing protease. Only Bacillus sp D7 strain was capable of producing three digestive enzymes (protease, amylase, cellulase) with hydrolyzed hollows ranged from 15.5–18.5 mm. The antibacterial activity of 9/16 test strains was positive against Salmonella Typhimurium, Staphylococcus aureus and Escherichia coli. Survivability of 15 test microorganisms in simulated gastrointestinal conditions was relatively high (c.a. 80 %). Three strains of L. acidophilus VAST, S. boulardii PLCP and Bacillus sp D7 when in mixture demonstrated a great potential as probiotics for animal.

Evaluation of Saccharomyces cerevisiae as an anti-fumonisin B1 additive in a horse digestion model

2017 ◽  
Vol 10 (2) ◽  
pp. 121-130
Author(s):  
F.M.P. Taran ◽  
V.P. Silva ◽  
L. Abrunhosa ◽  
C.A.R. Rosa ◽  
A. Venâncio ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Crude Protein ◽  
Liquid Fraction ◽  
Fumonisin B1 ◽  
In Vitro Digestion ◽  
Enzymatic Digestion ◽  
In Vitro Assays ◽  
Yeast Strains ◽  
Maize Grain

In this study, the capacity of Saccharomyces cerevisiae to adsorb fumonisin B1 (FB1) was evaluated in in vitro assays. The digestion of nutrients from maize contaminated with FB1 was assessed as well as the influence of digestive enzymes and pH on the bioavailability of FB1 in solution. Adsorption assays in buffers containing 5 µg/ml of FB1 were conducted to determine the strain to be used in the in vitro digestion assays. Four different yeast strains (1, 2, 3 and 4) along with five different cell concentrations of each one were studied under pH 2 and 6.8 at 39 °C. Strain 4 showed higher adsorption values at 1×109 cfu/ml, adsorbing 39.4% of the mycotoxin at pH 2 and 37.5% at pH 6.8. After that, the in vitro enzymatic digestion was conducted in two separated experiments. First, maize artificially contaminated with FB1 (5 µg/g) was used in five different treatments. Then, assays with maize naturally contaminated with FB1 (Maize A: 3.2 µg/g and Maize B: 29.0 µg/g) were conducted. In all samples, FB1 was quantified by HPLC-FL in liquid fraction and in solid residue. Samples of maize in natura and solid residues were subjected to chemical analysis of dry matter, organic matter, crude protein, neutral detergent fibre and starch to estimate the digestion of nutrients. The presences of FB1 and S. cerevisiae (Strain 4) in these assays had no influence on the digestion of the maize nutrients. The adsorption capacity of yeast was observed more clearly in treatments with higher concentrations of FB1 in the maize grain. S. cerevisiae strain 4 removed between 8 to 18% of FB1 in solution, showing a limited capacity to adsorb FB1 under in vitro conditions of horse enzymatic digestion.

scholarly journals Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 724 ◽  
Author(s):  
Agnieszka Wilkowska ◽  
Joanna Berlowska ◽  
Adriana Nowak ◽  
Ilona Motyl ◽  
Aneta Antczak-Chrobot ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Biomass Conversion ◽  
Lactobacillus Brevis ◽  
Feed Additives ◽  
Sugar Beet Pulp ◽  
Gastrointestinal Bacteria ◽  
Beet Pulp

An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by in vitro fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial Lactobacillus brevis strains, whereas pathogenic bacteria and yeast (C. albicans, C. lusitanie, C. pelliculosa), responsible for infections in breeding animals, showed much weaker adhesion.

scholarly journals Investigation and Biological Control of Toxigenic Fungi and Mycotoxins in Dairy Cattle Feeds

2020 ◽  
Author(s):  
Reem Moath Alasmar ◽  
Samir Jaoua
Keyword(s):  
Biological Control ◽  
Animal Feed ◽  
Fermented Food ◽  
Fungal Metabolites ◽  
Toxigenic Fungi ◽  
Yeast Strains ◽  
Mycotoxigenic Fungi ◽  
Food And Feed ◽  
Cattle Feeds

Mycotoxins, the secondary fungal metabolites are important contaminants of food and feed. Among the other contaminants, aflatoxin B1 (AFB1) and OTA are frequently detected in the animal feed product. In the present study, the mixed dairy cow feed products were collected from the supermarkets in Qatar and analyzed for the presence of AFB1 and OTA. Yeast strains were isolated and tested for their biological control activities against aflatoxigenic and ochratoxin fungi. We demonstrated that local 15 yeasts isolates have important antifungal potential activities through the synthesis of volatile organic compounds (VOC) that are able to act against the mycotoxigenic fungi and their synthesis of the mycotoxins. Two Yeast strains (4&2) isolated from fermented food, have shown a great antifungal inhibition growth in-vitro as well as spores inhibition and mycotoxins synthesis.

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