Gliotoxin natural incidence and toxigenicity of Aspergillus fumigatus isolated from corn silage and ready dairy cattle feed

2008 ◽  
Vol 1 (4) ◽  
pp. 457-462 ◽  
Author(s):  
C. Pereyra ◽  
V. Alonso ◽  
C. Rosa ◽  
S. Chiacchiera ◽  
A. Dalcero ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Aspergillus Fumigatus ◽  
Corn Silage ◽  
Cattle Feed ◽  
Feed Source ◽  
Apoptotic Effects ◽  
Penicillium Spp ◽  
Feed Samples ◽  
Natural Incidence

Corn silage is an important feed source for dairy and beef cattle in central Argentina. The presence of thermophilic species Aspergillus fumigatus is among the major problem in silage, as many strains can produce several mycotoxins that affect the health of dairy cattle. The aims of the present study were to determine total fungal counts and relative density of A. fumigatus in silage samples, and to determine the natural incidence of gliotoxin in silage and ready dairy feed samples. The potential gliotoxin production of A. fumigatus isolated from silage was also recorded. A total of 90 samples were investigated, which were taken immediately after opening of the silo and the end of the ensiling period of about after 5 months. Sampling was performed manually through silos in transects at 3 levels per silo. Thirty samples of ready cattle feed (corn silage, ground corn, barley, cotton seed, brewer grains) were collected and investigated as well. Gliotoxin contamination was determined by HPLC. The ability of A. fumigatus to produce gliotoxin was measured using a TLC method. Results show that in all samples, the total number of yeasts and moulds exceeded 1×104 cfu/g. Aspergillus was the most prevalent genus followed by Fusarium and Penicillium spp. Investigations of the isolated A. fumigatus strains showed that many of these strains produced more than one mycotoxin. All samples showed gliotoxin contamination, but the strains isolated from ready cattle feed had higher gliotoxin levels than those isolated from corn silage. The gliotoxin concentrations found in this study exceeded the levels that are known to induce immunosuppressive and apoptotic effects in vitro. Although at present the oral bioavailability of gliotoxin in cattle is not known, the presented data suggest that its presence in feedstuffs could affect productivity and present a health risk for dairy cows.

scholarly journals Results of one-year investigations of the contamination of dairy cattle feed and raw milk with moulds and mycotoxins

2011 ◽  
Vol 27 (3) ◽  
pp. 985-992
Author(s):  
M. Skrinjar ◽  
D. Jakic-Dimic ◽  
N. Blagojev ◽  
V. Soso
Keyword(s):  
Dairy Cattle ◽  
Standard Method ◽  
Raw Milk ◽  
Corn Silage ◽  
Aflatoxin M1 ◽  
Cattle Feed ◽  
Milk Samples ◽  
One Year ◽  
Feed Samples

The contamination of 98 dairy cattle feed samples with moulds and mycotoxins (AB1, OTA, ZEA) was investigated. The contamination of 80 raw milk samples with aflatoxin M1 was also tested. Determination of total viable counts per 1g of feed was performed using Koch's standard method. The content of AB1, OTA and ZEA in feed, as well as aflatoxin M1 in raw milk samples, was determined by VICAM fluorimetric method. Results showed that 91% of all tested samples was contaminated with moulds, and total viable mould count per gram ranged from 10 (corn silage - spring) to 4.9 x 106 per gram (dried alfalfa - winter). The isolated moulds belong to 20 genera and 72 species. Results of mycotoxicological investigations showed that 42 samples of total 98 tested, were contaminated with mycotoxins. The most frequent was OTA (24 samples, 20.00 - 210.00 ?g/kg) followed with ZEA (12 samples, 250.00 - 980.00 ?g/kg) and AB1 (3 samples, traces - 18.50 ?g/kg). Aflatoxin M1 in raw milk samples wasn?t found.

scholarly journals Supplementation of Heit-Chrose into Dairy Cow Feed Improves in Vitro Rumen Fermentation

2015 ◽  
Vol 17 (2) ◽  
pp. 76
Author(s):  
Caribu Hadi Prayitno
Keyword(s):  
Dairy Cattle ◽  
Dairy Cow ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Cattle Feed ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
C 30 ◽  
Organic Minerals

The objective of the study was to evaluate the effect of Heit-Chrose (HC) supplementation using in vitro method on ruminal fermentation of dairy cattle. HC is a feed supplement containing allicin, saponin and organic minerals ( Se , Cr and Zn).  This research was conducted using completely randomized design, with 6 treatments and 4 replications. The treatments were : 1). C = dairy cattle feed (CP 15.38%, CF 23.38%, TDN 61.26%); 2). HC-0 = C+  organic minerals (0.3 ppm Se + 0.15 ppm Cr + 40 ppm Zinc-lysinat) + 0 ppm of HC; 3). HC-15= C + 15 ppm HC; 4).HC-30 =C+ 30 ppm of HC; 5). HC-45 =C+ 45 ppm of  HC; 6). HC-60 =C  + 60 ppm of HC. Data obtained were analyzed using analysis of variance of SPSS program. HC supplementation increased the DMD, OMD, VFA, but reduced  total gas,  methane and  protozoa count.  HC supplementation greater than  30 ppm  did not further improve ruminal fermentation. Supplementation at 30 ppm of  HC to dairy cow feed was the appropriate level to improve the efficiency of rumen fermentation. 

Adoption and Commercialization of Green Corn, Green Corn –Based Silage, Haylage and Ummb Production for Dairy Cattle in Cagayan Valley, Philippines

2019 ◽  
Vol 4 (4) ◽  
pp. 82-92
Author(s):  
Eva U. Cammayo ◽  
Nilo E. Padilla
Keyword(s):  
Dairy Cattle ◽  
Nutritive Value ◽  
Crop Residues ◽  
Smallholder Farmers ◽  
Corn Silage ◽  
Small Scale ◽  
Dairy Farmers ◽  
Feed Resources ◽  
Dry Spell ◽  
Dairy Animals

This research aimed to improve dairy production and increase the income of dairy farmers using locally available feed resources. Small-scale milk producers rely heavily on available feed resources in the locality which are either indigenous in the area or introduced species for feed and nutrition of their dairy cattle and buffalos. Their milk output depends mainly on seasonal fluctuations in the quality and quantity of natural forage. Crop residues such as corn stover and rice straw which are high in fiber but low in nutrients serve as a feed supplement and filler to the daily diets of dairy cattle and buffalos. Cagayan Valley is an ear of top corn and rice-producing region. The potential of crop residues as feed supplements or raw materials of dairy cattle/buffalo feed mix is great. But dairy farmers still face the scarcity problem of quality feed resources for dairy animals especially during the dry season. The supply of forage is very low during the dry spell. Inadequate feed mix and low nutritive value of feed mix result in low or no milk production. Producing green corn and ensiling it to produce green corn silage preserves and prolong the storage life of forages. In this way, a stable supply of feed mix for dairy animals is assured year-round. Type of Paper: Empirical. Keywords: adoption and commercialization, dairy industry, financial viability, green-corn silage production, indigenous grasses, smallholder farmers.

scholarly journals HLA-DR Alpha 2 Mediates Negative Signalling via Binding to Tirc7 Leading to Anti-Inflammatory and Apoptotic Effects in Lymphocytes In Vitro and In Vivo

PLoS ONE ◽  
2008 ◽  
Vol 3 (2) ◽  
pp. e1576 ◽  
Author(s):  
Grit-Carsta Bulwin ◽  
Stephanie Wälter ◽  
Mirko Schlawinsky ◽  
Thomas Heinemann ◽  
Anke Schulze ◽  
...  
Keyword(s):  
Hla Dr ◽  
Anti Inflammatory ◽  
Apoptotic Effects

Exploring the Colonic Metabolism of Grape and Strawberry Anthocyanins and Their in Vitro Apoptotic Effects in HT-29 Colon Cancer Cells

2016 ◽  
Vol 65 (31) ◽  
pp. 6477-6487 ◽  
Author(s):  
María-Carmen López de las Hazas ◽  
Juana I. Mosele ◽  
Alba Macià ◽  
Iziar A. Ludwig ◽  
María-José Motilva
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Apoptotic Effects ◽  
Ht 29 ◽  
Colonic Metabolism

scholarly journals Pharmacodynamics of Itraconazole against Aspergillus fumigatus in anIn VitroModel of the Human Alveolus: Perspectives on the Treatment of Triazole-Resistant Infection and Utility of Airway Administration

2012 ◽  
Vol 56 (8) ◽  
pp. 4146-4153 ◽  
Author(s):  
Zaid Al-Nakeeb ◽  
Ajay Sudan ◽  
Adam R. Jeans ◽  
Lea Gregson ◽  
Joanne Goodwin ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Therapeutic Strategies ◽  
Wild Type ◽  
Content Type ◽  
Exposure Response ◽  
Prevention And Treatment ◽  
Resistant Infection ◽  
Resistant Strains ◽  
Type Strains

ABSTRACTItraconazole is used for the prevention and treatment of infections caused byAspergillus fumigatus. An understanding of the pharmacodynamics of itraconazole against wild-type and triazole-resistant strains provides a basis for innovative therapeutic strategies for treatment of infections. Anin vitromodel of the human alveolus was used to define the pharmacodynamics of itraconazole. Galactomannan was used as a biomarker. The effect of systemic and airway administration of itraconazole was assessed, as was a combination of itraconazole administered to the airway and systemically administered 5FC. Systemically administered itraconazole against the wild type induced a concentration-dependent decline in galactomannan in the alveolar and endothelial compartments. No exposure-response relationships were apparent for the L98H, M220T, or G138C mutant. The administration of itraconazole to the airway resulted in comparable exposure-response relationships to those observed with systemic therapy. This was achieved without detectable concentrations of drug within the endothelial compartment. The airway administration of itraconazole resulted in a definite but submaximal effect in the endothelial compartment against the L98H mutant. The administration of 5FC resulted in a concentration-dependent decline in galactomannan in both the alveolar and endothelial compartments. The combination of airway administration of itraconazole and systemically administered 5FC was additive. Systemic administration of itraconazole is ineffective against Cyp51 mutants. The airway administration of itraconazole is effective for the treatment of wild-type strains and appears to have some activity against the L98H mutants. Combination with other agents, such as 5FC, may enable the attainment of near-maximal antifungal activity.

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