Influence of exhaustive aerobic exercise on some cytokines and serum iron parameters in canine experimental model

2016 ◽  
Vol 12 (2) ◽  
pp. 83-89 ◽  
Author(s):  
P. Dzhelebov ◽  
D. Gundasheva ◽  
M. Andonova ◽  
V. Tsoneva ◽  
P. Marutsov ◽  
...  
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Serum Levels ◽  
Exhaustive Exercise ◽  
Moderate Intensity ◽  
Control Group ◽  
Tnf Α ◽  
Factor Α

The aim of the experiment was to study the effect of exhaustive exercise on some cytokines and iron status parameters. We used 12 male, mongrel dogs divided into two groups – animals from experimental group were submitted to exercise at moderate intensity with exhaustion as the end-point; animals from control group did no exercise. Serum levels of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), hepcidin prohormone, serum iron (SI), total iron binding capacity (TIBC) and transferrin saturation (TS) were measured before exercise (BE), right after exercise (0 hour) and on 2, 4, 24, 48 and 72 hours after exercise. SI, TIBC and TS were measured also on day 7 and 14 after exercise. Serum levels of TNF-α increased after the exhaustive exercise. Serum levels of IL-6 demonstrated an increase at 0 hour, but increase was not statistically significant compared to BE level. Serum levels of hepcidin prohormone marked a slight increase 48 hours after the exercise, but change was insignificant. Levels of SI decreased on hour 72 (P<0.01) and on day 7 (P<0.01) and 14 (P<0.05) after the exercise, as compared to BE level. Similar were changes in TS. TIBC decreased on 4, 24 and 72 hours (P<0.05) after exercise, but only compared to control group. In conclusion, exhaustive exercise causes inflammatory response and a significant decrease in SI levels.

scholarly journals IRON STATUS IN PREECLAMPSIA

2008 ◽  
Vol 15 (01) ◽  
pp. 74-80
Author(s):  
TASNEEM ZAFAR ◽  
ZAFAR IQBAL
Keyword(s):  
Serum Ferritin ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Correlation Study ◽  
Total Iron ◽  
Control Group ◽  
Iron Binding ◽  
Iron Binding Capacity

Objective: To evaluate iron status in pregnancy induced hypertension and role of iron in the etiologyand pathogenesis of pre-eclampsia. Design: Coefficient correlation study. Place and Duration. At Department ofBiochemistry, Frontier Medical College, Abbottabad with collaboration of Department of Obstetrics and Gynecology,Ayub Medical Complex, Abbottabad from March 2006-March 2007. Material and Methods: Study was performed onhundred pregnant women of age ranging between 15-35 years and having gestational age between28 to 34 weeks.Fifty obstetric patients were identified as having pre-eclampsia. Fifty healthy pregnant subjects were taken as controls,having uncomplicated pregnancies and were normotensive throughout gestation and without proteinuria. Results:Results depicts that mean age of pre-eclamptic group was significantly low (P<0.001) as compared to control. Bothparameters, Hemoglobin and Haematocrit were significantly higher (P<0.05) in pre-eclamptic as compared to controls.Serum iron, serum ferritin and transferrin saturation were significantly higher (P<0.001) in pre-eclamptic in comparisonwith control group. Total iron binding capacity and unsaturated iron binding capacity were significantly lower (P<0.001)in pre-eclamptic group when compared to control group. Correlation coefficient between serum iron, total iron bindingcapacity (TIBC), serum ferritin, unsaturated iron binding capacity (UIBC) and systolic and diastolic blood pressure inpre-eclamptic group showed no significant positive correlation in any parameter. Conclusion: It is concluded thathemoglobin, haematocrit, serum iron, serum ferritin and transferrin saturation are significantly increased in pregnantwomen that later develops pre-eclampsia. Excess iron is postulated as casual factor in the oxidative stress ie; in itsradical form, which may be involved in the pathogenesis of pre-eclampsia. Therefore, iron status of pregnant womenshould be assessed before giving iron supplements as these may cause more harm than benefit.

Melatonin Improves Erythropoietin Hyporesponsiveness via Suppression of Inflammation

2019 ◽  
Vol 14 (3) ◽  
pp. 203-208
Author(s):  
Evan Noori Hameed ◽  
Haydar F. Hadi AL Tukmagi ◽  
Hayder Ch Assad Allami
Keyword(s):  
Iron Status ◽  
Transferrin Saturation ◽  
Control Group ◽  
Base Line ◽  
Inadequate Response ◽  
Inflammatory Parameters ◽  
Tnf Α ◽  
Before And After ◽  
And Control ◽  
Controlled Clinical Study

Background: Inadequate response to Erythropoietin Stimulating Agents (ESA) despite using relatively larger doses regimen represents a potential risk factor of Cardiovascular (CV) related mortality in addition to health-care economic problems in anemic patients with Chronic Kidney Disease (CKD). Erythropoietin (EPO) hyporesponsiveness related to inflammation has been increased progressively. Melatonin is well known as a potent anti-inflammatory agent. Therefore, the current study was designed to evaluate whether melatonin could improve anemic patients response to EPO. Methods: This single controlled clinical study was carried out in 41 CKD patients with hemoglobin (Hb) levels less than 11g/dl divided randomly in a 1:1 ratio into 2 groups; treatment group who received 5mg melatonin plus their regular treatments and control group who received their regular treatments only. Hematological and iron status parameters include Hb level, serum iron (S. iron), Transferrin Saturation Ratio (TSAT) and serum ferritin (S. ferritin) in addition to inflammatory parameters that include tissue necrotic factor alfa (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) determined before and after 12 weeks of treatment. Results: Melatonin remarkably increases the Hb level with a significant increase in S. iron and TSAT compared to baseline. The elevation of S. iron and TSAT was significantly higher in the melatonin group. Additionally, all inflammatory markers estimated were reduced significantly by melatonin compared to base line and control group. Conclusion: The results of the current study showed that melatonin has an advantageous effect on improving EPO response in anemic patients with CKD.

scholarly journals Application of Dexmedetomidine in Cardiopulmonary Bypass Prefilling

Dose-Response ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 155932582093976 ◽  
Author(s):  
Li Wang ◽  
Shaowei Wang ◽  
Zhen Xing ◽  
Fulong Li ◽  
Jinliang Teng ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Anesthesia Induction ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Objective: The purpose of this study was to explore the application of dexmedetomidine (Dex) in cardiopulmonary bypass. Methods: A total of 60 patients undergoing elective cardiopulmonary bypass were divided into control (C) group and Dex group. In the Dex group, appropriate amount of Dex was added into the membrane lung prefilling solution before anesthesia induction, while those in control group were given normal saline. The levels of mean arterial pressure (MAP) and heart rate (HR) at different times were measured. The levels of cardiac troponin I (CTNI), malondialdehyde (MDA), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) at different points (T0/T1/T2/T3/T4) in both groups were measured by enzyme-linked immunosorbent assay kits. Results: The intraoperative and postoperative levels of MAP and HR in the 2 groups were significantly lower than those preoperatively ( P < .05). The levels of MAP and HR in the Dex group were significantly lower than those of the C group ( P < .05). The levels of CTNI/MDA/IL-6/TNF-α at different points in both groups were significantly higher than those at T0 ( P < .05). The serum levels of CTNI, MDA, IL-6, and TNF-α in the Dex group at T1/T2/T3/T4 were significantly lower than those in the C group ( P < .05). The rate of arrhythmia in the Dex group was significantly lower than that in the C group ( P < .05). Conclusion: Dexmedetomidine has a stable effect in cardiopulmonary priming solution.

Interaction Between Genotypes for HFE and TFR2 Genes Mutations and Iron Status in Brazilian Blood Donors

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5382-5382
Author(s):  
Rodolfo D Cancado ◽  
Paulo CJL Santos ◽  
Samuel Rostelato ◽  
Cristiane T Terada ◽  
Iris Gonzales ◽  
...  
Keyword(s):  
Serum Ferritin ◽  
Serum Iron ◽  
Blood Donors ◽  
Binding Capacity ◽  
Iron Status ◽  
Hereditary Hemochromatosis ◽  
Transferrin Saturation ◽  
The Body ◽  
Hfe Gene ◽  
Automation System

Abstract Hereditary hemochromatosis (HH) is a disorder characterized by increased intestinal iron absorption, which leads to a progressive accumulation of iron in the body. This iron overload has been associated with mutations in HFE gene (C282Y, H63D and S65C) and other genes. The objectives of this study were to assess the frequencies of functional mutations in HFE and TFR2 genes and to investigate their relationship with the iron status in a sample of blood donors. Blood donors (n=542) were recruited at the Hemocenter of the Santa Casa Hospital, Sao Paulo, Brazil. The genotypes for HFE (C282Y, H63D and S65C) TFR2 (Y250X and Q690P) gene mutations were evaluated by PCR-RFLP. The concentrations of serum iron and total iron-binding capacity (TIBC) were measured by automation system Advia®(Bayer Diagnostics) and serum ferritin by Axsym System®(Abbott Laboratories). The frequencies of HFE 282Y, HFE 63D and HFE 65C alleles were 2.1, 13.6 and 0.6%, respectively. The frequency C282Y allele (2.1%) in Brazilian blood donors is lower than that observed in blood donors from Northern Europe (5.1 to 8.2%, P&lt;0.05). The TFR2 250X and TFR2 690P alleles were not found in these subjects. The iron status was similar between HFE genotypes in women. However, men carrying HFE 282CY genotype had higher serum ferritin and lower TIBC concentrations when compared to the HFE 282CC genotype carriers. HFE 282CY genotype was also associated with higher transferrin saturation in men who donated blood at the first time. Moreover, male donors with HFE 63DD plus 63HD genotypes had higher serum iron and transferrin saturation when compared to those with HFE 63HH genotype. A relationship between HFE CY/HH/SS haplotype and lower TIBC concentrations was also found in men. The HFE 282Y and HFE 65C alleles were rare while the HFE 63D was frequent in blood donors. The mutations in TFR2 gene were not found in this study. The HFE 282Y and HFE 63D alleles were associated with alterations on iron status only in male blood donors.

Siltuximab Treatment Increases Hemoglobin (Hb) Levels: Preliminary Results From a Prospective Phase 1 Study In Refractory Solid Tumors

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 5150-5150
Author(s):  
R. Kurzrock ◽  
E. Angévin ◽  
S. Cohen ◽  
J. Van Laethem ◽  
B. Rijnbeek ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Research Funding ◽  
Serum Iron ◽  
Binding Capacity ◽  
Iron Status ◽  
Phase 1 ◽  
Transferrin Saturation ◽  
Maximum Increase ◽  
Baseline Serum ◽  
Clinically Significant

Abstract Abstract 5150 Introduction: Siltuximab is a chimeric monoclonal antibody with high affinity for the inflammatory cytokine, IL-6, which is currently being studied in hematologic, solid malignancies and multicentric Castleman's disease (MCD). In addition to representing a therapeutic target, IL-6 is reported to play a key role in the etiology and symptoms of anemia of cancer. A possible mechanism is through up-regulation of hepatic production of hepcidin, the central iron-regulatory hormone. Siltuximab treatment has previously been shown to be associated with clinically significant Hb increases in MCD (a disorder caused by deregulated IL-6 production) and renal cell carcinoma. We have prospectively studied the Hb response in the context of a phase I study with siltuximab in patients with advanced solid tumors. Patients and Methods Siltuximab was administered intravenously to patients with any advanced solid tumor at increasing dose levels (2.8 or 5.5 mg/kg every 2 weeks, 11 or 15 mg/kg every 3 weeks). Hepcidin (C-ELISA), Hb, CRP (marker for inflammation) and iron status (serum iron, ferritin, transferrin saturation, total iron binding capacity) were measured at baseline and serially during treatment. IL-6 was not measured since interference of the drug with assay performance prevents accurate measurement of bioactive IL-6. The relationships between these biomarkers and Hb response (defined as a maximum Hb increase of ≥1 g/dL during treatment) were evaluated. Results: Forty-four pts (18 colorectal, 12 ovarian, 5 pancreatic, 9 other) received a median of 3 siltuximab cycles (range 1 – 25). Eight patients were excluded from analysis because they received blood transfusions or ESAs. There were no objective tumor responses (CR or PR). Baseline Hb ranged from 9.4–15.3 g/dL (median 12.2). All 36 evaluable patients had an increase in Hb (median 1.35 g/dL; range 0.1–3.2). Eleven (31%) patients had a maximum increase of ≥2 g/dL. Maximum Hb levels did not exceed the upper limit of normal. Baseline hepcidin (median 118.6 ng/mL; range 9.5–493.3) was positively correlated with baseline CRP (median 13.6 mg/L; range 0.42–152.0) (p<0.05) and ferritin (median 346 pmol/L; range 74.2–8543.1) (p<0.05) but not with baseline Hb or Hb response. For subjects with a Hb response of more than 2 g/dL an association was found with Day 8 hepcidin (p = 0.03) when controlled for baseline serum iron. Early hepcidin percentage change was not correlated with Hb response. Conclusion: Siltuximab treatment was associated with clinically meaningful Hb response in this moderately anemic refractory cancer population. The exact mechanism of action remains uncertain, however correlation with additional markers (e.g., soluble transferrin receptor, inflammatory markers such as IL-6) might also be important to identify patients most likely to respond to treatment and should be evaluated further in randomized trials. Disclosures: Kurzrock: Johnson & Johnson: Research Funding. Angévin:Johnson & Johnson: Research Funding. Cohen:Johnson & Johnson: Research Funding. Van Laethem:Johnson & Johnson: Research Funding. Rijnbeek:Johnson & Johnson: Employment. Vermeulen:Johnson & Johnson: Employment. Tromp:Johnson & Johnson: Employment. Li:Johnson & Johnson: Employment. Reddy:Johnson & Johnson: Employment. Cornfeld:Johnson & Johnson: Employment. Tabernero:Johnson & Johnson: Research Funding.

scholarly journals Low serum iron is associated with anemia in CKD stage 1–4 patients with normal transferrin saturations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pei-Hua Yu ◽  
Ming-Yen Lin ◽  
Yi-Wen Chiu ◽  
Jia-Jung Lee ◽  
Shang-Jyh Hwang ◽  
...  
Keyword(s):  
Serum Iron ◽  
Reference Group ◽  
Binding Capacity ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Reactive Protein ◽  
Ckd Stage ◽  
Adjusted Logistic Regression ◽  
Stage 1 ◽  
Low Serum

AbstractLow transferrin saturation (TSAT), calculated by serum iron divided by total iron-binding capacity (TIBC), indicates iron deficiency. Because malnutrition and inflammation are associated with low TIBC in chronic kidney disease (CKD), TSAT might not reflect iron status or risk for anemia. We examined whether low serum iron was a risk factor for anemia in CKD patients with normal TSAT. Thus we compare the risk for anemia in 2500 CKD stage 1–4 patients divided by TSAT (cutoff: 20%) and serum iron (cutoff: 70 μg/dL in men, 60 μg/dL in women). Our results confirmed low TIBC (< 200 μg/dL) was associated with hypoalbuminemia and high C-reactive protein. In fully-adjusted logistic regression, both “normal TSAT low iron” and “low TSAT low iron” groups were associated with baseline anemia (hemoglobin < 11 g/dL) (odds ratios (OR) 1.56; 95% confidence interval (CI) 1.13–2.16 and OR 2.36; 95% CI 1.76–3.18, respectively) compared with the reference group (normal TSAT normal iron). Sensitivity tests with different cutoffs for TSAT and iron also showed similar results. In patients without anemia, both groups were associated with anemia after 1 year (OR 1.69; 95% CI 1.00–2.83 and OR 1.94; 95% CI 1.11–3.40, respectively). In conclusion, CKD stage 1–4 patients with normal TSAT but low serum iron are still at risk for anemia.

Effect of Hypothermia on Serum Myelin Basic Protein and Tumor Necrosis Factor-α in Neonatal Hypoxic-Ischemic Encephalopathy

2021 ◽  
Author(s):  
Qiuli Wang ◽  
Hongyan Lv ◽  
Sujing Wu ◽  
Junxia Song ◽  
Junqin Li ◽  
...  
Keyword(s):  
Tumor Necrosis ◽  
Basic Protein ◽  
Serum Levels ◽  
Control Group ◽  
Tnf Α ◽  
Hypothermia Group ◽  
Neurodevelopmental Impairment ◽  
Factor Α ◽  
Ischemic Encephalopathy ◽  
Necrosis Factor

Objective Multiple randomized controlled trials have shown that hypothermia is a safe and effective treatment for neonatal moderate or severe hypoxic-ischemic encephalopathy (HIE). The neuroprotective mechanisms of hypothermia need further study. The aim of this study was to investigate the effect of hypothermia on the serum levels of myelin basic protein (MBP) and tumor necrosis factor-α (TNF-α) as well as neurodevelopmental outcomes in neonatal HIE. Study Design Eighty-five neonates with moderate-to-severe HIE were divided into a hypothermia group (n = 49) and a control group (n = 36). Serum levels of MBP and TNF-α within 6 hours after birth and after 3 days of treatment were determined by enzyme-linked immunosorbent assay, and neurodevelopmental outcome at the age of 12 to 15 months was assessed by using the Gesell development scale. Results After 3 days of treatment, serum levels of MBP and TNF-α in the control group were not significantly different from levels before treatment (p > 0.05), and serum levels of MBP and TNF-α in the hypothermia group were significantly lower than levels before treatment (p < 0.05). Serum levels of MBP and TNF-α were significantly negatively correlated with developmental quotient (DQ; r =  − 0.7945, p = 0.0000; r =  − 0.7035, p = 0.0000, respectively). Serum levels of MBP and TNF-α in neurodevelopmentally impaired infants were significantly higher than those in infants with suspected neurodevelopmental impairment and those in neurodevelopmentally normal infants (both p < 0.01). The rate of reduction of neurodevelopmental impairment was higher among infants in the hypothermia group than among those in the control group (χ2 = 16.3900, p < 0.05). Conclusion Hypothermia can reduce serum levels of MBP and TNF-α in neonates with HIE. Inhibiting the release of TNF-α may be one of the mechanisms by which hypothermia protects the myelin sheath. Key Points

scholarly journals Serum iron and serum ferritin levels in cholelithiasis: a randomized study

2018 ◽  
Vol 5 (4) ◽  
pp. 1411
Author(s):  
Bhavinder Kumar Arora ◽  
Anuj Kumar Yadav
Keyword(s):  
Serum Ferritin ◽  
Serum Iron ◽  
Iron Status ◽  
Gallstone Disease ◽  
Transferrin Saturation ◽  
Control Group ◽  
Case Group ◽  
Iron Store ◽  
Female Population ◽  
Low Serum

Background: The role of iron in the pathogenesis of gallstone disease has not been well established so far. Iron deficiency has been shown to alter the activity of several hepatic enzymes, leading to increased cholesterol saturation of bile in gall bladder and hence promoting cholesterol crystallization.Serum iron, total iron binding capacity and transferrin saturation are not good indicators of iron status in individuals. In infection free situation, serum ferritin is an ideal indicator for diagnosis of iron deficiency.Methods: The study was conducted as a prospective study in the department of Surgery. The study population was divided into two groups; Case group with 200 patients with gallstone disease and control group with 50 patients without gallstone disease. Serum iron and ferritin contents of both groups will be analyzed and compared with each other.Results: In this study the gallstones are more prevalent in female population than males in ratio of 5.4:1. Serum iron in males was low in 41.93% not comparable 20.8% of control suggesting that low serum iron is not associated with Cholelithiasis in male. In males, serum ferritin was low in 64.5% of cases and 16.66% of controls. Serum ferritin levels were normal in 35.50% of cases and 66.66% of controls and above normal in 16.66% of controls suggesting that low serum ferritin is associated with gall stones in males. In this study, low serum iron was seen in 23.07% of females comparable to 23% low serum iron in control females and low ferritin was seen in 35.50% of female cases as compare to 15.38% of controls.Conclusions: It was concluded that a low body store of serum iron is a risk factor for cholelithiasis in females and serum iron, serum ferritin may be used as marker of iron store so that low serum iron status could be diagnosed at early stage.

scholarly journals CHANGES IN THE PARAMETERS OF IRON METABOLISM IN RATS’ BLOOD UNDER DECIMETRIC ELECTROMAGNETIC RADIATION

2019 ◽  
Vol 64 (3) ◽  
pp. 274-282
Author(s):  
M. T. Abbasova ◽  
A. M. Gadzhiev
Keyword(s):  
Electromagnetic Radiation ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Iron Binding ◽  
Decimeter Range ◽  
Iron Binding Capacity ◽  
And Control

Introduction. The intensive development of radio and electrical communications, as well as various electronic devices, leads to the electromagnetic pollution of the environment.Aim. In this work, the authors set out to study the serum iron parameters of rats exposed to chronic electromagnetic radiation (EMR) of the decimeter range.Materials and methods. The research was carried out on rats that were divided into experimental and control groups. The experimental group was further divided into 4 subgroups of 10 animals each, which were subsequently exposed to electromagnetic radiation at a frequency of 460 MHz (Volna-2 apparatus) for 1, 2, 3 and 4 weeks. The control group (10 rats) was exposed to pretend irradiation, with the device being turned off. The following parameters were estimated: serum iron (SI), total iron-binding capacity (TIBC) and unsaturated iron-binding capacity (UIBC) of serum, transferrin saturation (TS), as well as serum concentrations of transferrin, haptoglobin, malondialdehyde and lipid hydroperoxides.Results. Differences in the SI concentration were found in the subgroups of animals exposed to radiation for 3 and 4 weeks (44.1 ± 3.1 μmol/l and 56.8 ± 4.4 μmol/l, respectively), as compared to the control group (30.5 ± 3.3 μmol/l). In experimental animals, TIBC increased by 41 % (p <0.05) — relative to the control group (110.8 ± 10.1 μmol/l) — only following 3 weeks of irradiation (156.2 ± 18.2 μmol/l), with a decrease in TIBC to 123.6 ± 16.4 μmol/l being noted during the 4th week. The concentration of transferrin increased from 45.6 ± 8.0 μmol/l (control) to 81.0 ± 11.5 μmol/l during the 3rd week of radiation exposure, with a decrease to 55.9 ± 6.7 μmol/l being observed during the 4th week. TS increased from 27.5 % (control) to 45.9 % only following 4 weeks of irradiation. The content of lipid hydroperoxides and malondialdehyde in the blood of irradiated rats was higher, as compared to the control animals. The serum concentration of haptoglobin amounted to 26.7 % in the control group, reaching 53.8 mg % and 47.8 mg % following 3 and 4 weeks of exposure, respectively.Conclusion. The total chronic exposure to decimetric EMR produces an oxidising effect on organisms. 

Sign in / Sign up

Export Citation Format

Share Document