Bacillus toyonensis improves immune response in the mice vaccinated with recombinant antigen of bovine herpesvirus type 5

2018 ◽  
Vol 9 (1) ◽  
pp. 133-142 ◽  
Author(s):  
F.D.S. Santos ◽  
Y.A. Menegon ◽  
R.E.A. Piraine ◽  
P.R.C. Rodrigues ◽  
R.C. Cunha ◽  
...  
Keyword(s):  
Immune Response ◽  
Animal Feed ◽  
Bovine Herpesvirus ◽  
Experimental Period ◽  
Recombinant Vaccine ◽  
Recombinant Vaccines ◽  
Recombinant Glycoprotein ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5 ◽  
Bacillus Toyonensis

Probiotics modulate the immune response and can increase the effectiveness of vaccines. Bacillus toyonensis is widely used as a probiotic in animal feed. The aim of this study was to assess the effects of B. toyonensis administration on the immune response to an experimental recombinant vaccine against bovine herpesvirus type 5 (BoHV-5) in mice. Mice were vaccinated with BoHV-5 recombinant glycoprotein D and supplemented with the probiotic B. toyonensis in two regimes: one group received the probiotic only during seven days prior to the initial vaccination while the second group was given the probiotic throughout the experimental period of seven weeks. Animals supplemented with probiotic B. toyonensis in two regimes showed an increase in total immunoglobulin (Ig)G, IgG1 and IgG2a levels in serum, in addition to higher titres of antibodies capable of neutralising the BoHV-5 virus than non-supplemented animals (P<0.05). Splenocytes from the supplemented mice had higher mRNA transcription levels of cytokines interleukin (IL)-4 and IL-12. These results show that the use of this probiotic may significantly contribute to the response elicited by recombinant vaccines, especially those that rely on increasing antibody and cell-mediated immune responses for efficacy. Further, the data support an immunomodulatory effect for probiotic B. toyonensis and imply that enhance effect on the immune response against a BoHV-5 recombinant vaccine in mice.

Probiotics Bacillus toyonensis and Saccharomyces boulardii improve the vaccine immune response to Bovine herpesvirus type 5 in sheep

2018 ◽  
Vol 117 ◽  
pp. 260-265 ◽  
Author(s):  
Talita Bandeira Roos ◽  
Carina Martins de Moraes ◽  
Régis Tuchtenhagen Sturbelle ◽  
Luana Alves Dummer ◽  
Geferson Fischer ◽  
...  
Keyword(s):  
Immune Response ◽  
Bovine Herpesvirus ◽  
Saccharomyces Boulardii ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5 ◽  
Bacillus Toyonensis

scholarly journals Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

2018 ◽  
Vol 70 (2) ◽  
pp. 375-381 ◽  
Author(s):  
T.B. Roos ◽  
L.F.C. Avila ◽  
R.T. Sturbelle ◽  
F.L.L. Leite ◽  
G. Fischer ◽  
...  
Keyword(s):  
Immune Response ◽  
Animal Health ◽  
Bovine Herpesvirus ◽  
Saccharomyces Boulardii ◽  
Igg Antibody ◽  
Th1 Response ◽  
Herpesvirus Type ◽  
Cell Mediated Immune Response ◽  
A Cell ◽  
Bovine Herpesvirus Type 5

ABSTRACT There have been significant efforts towards the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics to enhance the immune response of the host, leading to increased immunogenicity of antigen preparations. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotic Saccharomyces boulardii (Sb) as a potential adjuvant to improve vaccine efficiency. We found that the supplemented animals exhibited an enhanced systemic IgG antibody response toward a Th1 response in favor of IgG2a and increased mRNA expression levels of the cytokines IFN-y, IL-12, IL-17 and IL-10 in the spleen. These results suggest that Sb supplementation may provide a promising means for improving the efficiency of vaccines, particularly those that rely on a cell-mediated immune response.

Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5

2007 ◽  
Vol 116 (1-2) ◽  
pp. 79-84 ◽  
Author(s):  
Geferson Fischer ◽  
Marlete Brum Cleff ◽  
Luana Alves Dummer ◽  
Niraldo Paulino ◽  
Amarílis Scremin Paulino ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Bovine Herpesvirus ◽  
Adjuvant Effect ◽  
Herpesvirus Type ◽  
Humoral Immune ◽  
Bovine Herpesvirus Type 5

Immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5 as vaccine antigen

Vaccine ◽  
2018 ◽  
Vol 36 (50) ◽  
pp. 7708-7714 ◽  
Author(s):  
Itauá Leston Araujo ◽  
Luana Alves Dummer ◽  
Paulo Ricardo Centeno Rodrigues ◽  
Alceu Gonçalves dos Santos ◽  
Geferson Fischer ◽  
...  
Keyword(s):  
Immune Responses ◽  
Bovine Herpesvirus ◽  
Vaccine Antigen ◽  
Glycoprotein D ◽  
Recombinant Glycoprotein ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

2021 ◽  
Author(s):  
Alok Joshi ◽  
R.P. Gupta ◽  
Selvaraj Pavulraj ◽  
Bidhan Chandra Bera ◽  
Taruna Anand ◽  
...  
Keyword(s):  
Immune Response ◽  
Plasmid Dna ◽  
Protective Immune Response ◽  
Glycoprotein B ◽  
Equine Herpesvirus ◽  
Recombinant Glycoprotein ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Background: Equine herpesvirus type 1 (EHV-1) is the most important viral pathogen of equines, causing respiratory illness, abortion, neonatal foal mortality and neurologic disorders. Large numbers of commercial EHV-1 vaccines are available to protect equines from the disease, but they provide only partial protection. Despite immunization with inactivated and modified live virus vaccine, mares show abortions. Present study was aimed to investigate the immunogenicity and protective efficacy of EHV-1 recombinant glycoprotein B (rgB) and gB expressing plasmid DNA against EHV-1 infection in BALB/c mice model.Methods: About 3-4 weeks old 225 female BALB/c mice were selected for the comparative study of immunization followed by challenged with EHV-1/India/Tohana/96-2 strain virus in 5 different groups of 45 animals each.Result: Following immunization, rgB vaccinated mice showed optimal stimulation of EHV-1 gB specific cell mediated and humoral mediated immunity (HMI and CMI). The gB expressing plasmid DNA vaccinated mice developed only CMI while inactivated whole virus vaccinated mice had only HMI. Upon EHV-1 challenge, all infected mice displayed variable levels of clinical signs with changes in body weight, however, vaccinated mice showed very rapid recovery with optimal protection. Positive control group mice showed severe pulmonary lesions along with persistence virus infection till 5 days post challenge (dpc) whereas vaccinated mice had less pulmonary lesion only up to 3dpc. Minimal lung lesions and early virus clearance was observed in the rgB immunized mice in comparison to the gB plasmid DNA and inactivated EHV-1 vaccine immunized mice. It has been concluded that immunization with rgB elicits optimum protective immune response against EHV-1 infection in mice model. The rgB could be a potential vaccine candidate against EHV-1 infection in equine in the future.

scholarly journals Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5) gI, gE and US9-negative

2009 ◽  
Vol 29 (11) ◽  
pp. 913-918 ◽  
Author(s):  
Alessandra D'Avila Silva ◽  
Ana Cláudia Franco ◽  
Paulo Augusto Esteves ◽  
Fernando Rosado Spilki ◽  
Paulo Michel Roehe
Keyword(s):  
Neurological Disease ◽  
Age Groups ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Herpesvirus Type ◽  
Viral Genes ◽  
Bovine Herpesvirus Type 5 ◽  
Two Age Groups

Bovine herpesvirus type 5 (BoHV-5) is a major cause of viral meningoencephalitis in cattle. The expression of different viral proteins has been associated with BoHV-5 neuropathogenesis. Among these, gI, gE and US9 have been considered essential for the production of neurological disease in infected animals. To evaluate the role of gI, gE and US9 in neurovirulence, a recombinant from which the respective genes were deleted (BoHV-5 gI-/gE-/US9-) was constructed and inoculated in rabbits of two age groups (four and eight weeks-old). When the recombinant virus was inoculated through the paranasal sinuses of four weeks-old rabbits, neurological disease was observed and death was the outcome in 4 out of 13 (30.7 %) animals, whereas clinical signs and death were observed in 11/13 (84.6%) of rabbits infected with the parental virus. In eight weeks-old rabbits, the BoHV-5 gI-/gE-/US9- did not induce clinically apparent disease and could not be reactivated after dexamethasone administration, whereas wild type BoHV-5 caused disease in 55.5% of the animals and was reactivated. These findings reveal that the simultaneous deletion of gI, gE and US9 genes did reduce but did not completely abolish the neurovirulence of BoHV-5 in rabbits, indicating that other viral genes may also play a role in the induction of neurological disease.

scholarly journals Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

2012 ◽  
Vol 10 (1) ◽  
pp. 53 ◽  
Author(s):  
Mariana PC Brenner ◽  
Camila Silva-Frade ◽  
Marina C Ferrarezi ◽  
Andrea F Garcia ◽  
Eduardo F Flores ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Developmental Changes ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

Susceptibility of mice to bovine herpesvirus type 5 infection in the central nervous system

2017 ◽  
Vol 41 (4) ◽  
pp. 279-288 ◽  
Author(s):  
L. P. Mesquita ◽  
R. C. Costa ◽  
M. M. Fusuma ◽  
F. R. P. Bruhn ◽  
E. Mori ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
The Central Nervous System ◽  
Bovine Herpesvirus Type 5

In vitroantiviral activity of Brazilian plants (Maytenus ilicifoliaandAniba rosaeodora) against bovine herpesvirus type 5 and avian metapneumovirus

2012 ◽  
Vol 50 (10) ◽  
pp. 1269-1275 ◽  
Author(s):  
L. K. Kohn ◽  
C. L. Queiroga ◽  
M. C. Martini ◽  
L. E. Barata ◽  
P. S. S. Porto ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Avian Metapneumovirus ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5
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