Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes

2017 ◽  
Vol 8 (5) ◽  
pp. 809-822 ◽  
Author(s):  
J. Lukic ◽  
I. Jancic ◽  
N. Mirkovic ◽  
B. Bufan ◽  
J. Djokic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Mrna Expression ◽  
Lactococcus Lactis ◽  
Wistar Rats ◽  
Immunological Response ◽  
Listeriolysin O ◽  
Mesenteric Lymph Nodes ◽  
Lactobacillus Salivarius ◽  
Cd69 Expression ◽  
Immunological Effects

In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer’s patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1β and tumour necrosis factor (TNF)-α in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1β, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.

scholarly journals Expression of two Listeria monocytogenes antigens (P60 and LLO) in Lactococcus lactis and examination for use as live vaccine vectors

2010 ◽  
Vol 59 (8) ◽  
pp. 904-912 ◽  
Author(s):  
Mohammed Bahey-El-Din ◽  
Pat G. Casey ◽  
Brendan T. Griffin ◽  
Cormac G. M. Gahan
Keyword(s):  
Immune Response ◽  
Listeria Monocytogenes ◽  
Lactococcus Lactis ◽  
Protective Efficacy ◽  
Oral Immunization ◽  
Listeriolysin O ◽  
Oral Vaccination ◽  
Vaccine Vectors ◽  
Food Borne ◽  
Presentation Pathway

Listeria monocytogenes is a food-borne intracellular pathogen that mainly infects pregnant and immunocompromised individuals. The pore-forming haemolysin listeriolysin O (LLO), the main virulence factor of Listeria monocytogenes, allows bacteria to escape from the harsh environment of the phagosome to the cytoplasm of the infected cell. This leads to processing of bacterial antigens predominantly through the cytosolic MHC class I presentation pathway. We previously engineered the food-grade bacterium Lactococcus lactis to express LLO and demonstrated an LLO-specific CD8+ response upon immunization of mice with the engineered L. lactis vaccine strains. In the present work, we examined the immune response and protective efficacy of an L. lactis strain co-expressing LLO and a truncated form of the listerial P60 antigen (tP60). Oral immunization revealed no significant protection against listeriosis with L. lactis expressing LLO, tP60 or the combined LLO/tP60. In contrast, intraperitoneal vaccination induced an LLO-specific CD8+ immune response with LLO-expressing L. lactis but no significant improvement in protection was observed following vaccination with the combined LLO/tP60 expressing L. lactis strain. This may be due to the low level of tP60 expression in the LLO/tP60 strain. These results demonstrate the necessity for improved oral vaccination strategies using LLO-expressing L. lactis vaccine vectors.

Su.78. Analysis of T-Cell Responses to Listeria Monocytogenes Listeriolysin O in Healthy Adults

2006 ◽  
Vol 119 ◽  
pp. S186
Author(s):  
Johannes Hampl ◽  
Shruti Mathur ◽  
Weiqun Liu ◽  
Peter Lauer ◽  
Thomas Dubensky ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
T Cell ◽  
T Cell Responses ◽  
Healthy Adults ◽  
Listeriolysin O ◽  
Cell Responses

scholarly journals Volatile Molecule Profiles and Anti-Listeria monocytogenes Activity of Nisin Producers Lactococcus lactis Strains in Vegetable Drinks

2019 ◽  
Vol 10 ◽  
Author(s):  
Lorenzo Siroli ◽  
Lucia Camprini ◽  
Maria Barbara Pisano ◽  
Francesca Patrignani ◽  
Rosalba Lanciotti
Keyword(s):  
Listeria Monocytogenes ◽  
Lactococcus Lactis

scholarly journals Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits

2020 ◽  
Vol 65 (No. 1) ◽  
pp. 23-30 ◽  
Author(s):  
Heping Zhao ◽  
Feike Zhang ◽  
Jun Chai ◽  
Jianping Wang
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Listeriolysin O ◽  
Serum Iga ◽  
Probiotic Lactic Acid Bacteria ◽  
Spleen And Lymph Nodes ◽  
Proinflammatory Factors

The present study aimed to investigate the effect of probiotic lactic acid bacteria (LAB) addition on Listeria monocytogenes translocation and its toxin listeriolysin O (LLO), proinflammatory factors, immune organ indexes and serum immunoglobulins in farmed rabbits. Five treatments included negative control (NC), positive control (PC) with L. monocytogenes infection and supplemental LAB at 3.0 × 10<sup>6 </sup>(low-LAB, L-LAB), 3.0 × 10<sup>8</sup> (medium-LAB, M-LAB) and 3.0 × 10<sup>10 </sup>(high-LAB, H-LAB) CFU/kg of diet, respectively. The LAB was a mixture of equal amounts of Lactobacillus acidophilus (ACCC11073), Lactobacillus plantarum (CICC21863) and Enterococcus faecium (CICC20430). A total of 180 weaned rabbits (negative for L. monocytogenes) were randomly assigned to 5 groups with 6 replicates of 6 rabbits each in response to the 5 treatments. L. monocytogenes infection occurred on the first day of feeding trial and dietary LAB supplementation lasted for 14 days. The results showed that on days 7 and 14 post administration, L. monocytogenes in caecum, liver, spleen and lymph nodes was reduced in M-LAB and H-LAB compared to PC (P &lt; 0.05), and linear and quadratic reducing trends were found in liver on day 7 (P ≤ 0.002). On day 14, mucosa LLO mRNA expression and serum TNFα, IL1β and IFNγ were reduced in the three LAB treatments (P &lt; 0.05), and linear and quadratic trends were found on TNFα and IL1β (P ≤ 0.025); indexes of thymus and spleen, serum IgA and IgG were increased in the LAB treatments (P &lt; 0.05). It is concluded that LAB can be used to alleviate L. monocytogenes infection and to improve the immune function of farmed animals.

The immunological response of Wistar rats to the intracranially implanted C-6 glioma cell line

1983 ◽  
Vol 1 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Kang M. Chiu ◽  
Jules E. Harris ◽  
Jeffrey S. Kroin ◽  
William Slayton ◽  
Donald P. Braun
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Wistar Rats ◽  
Immunological Response ◽  
Glioma Cell Line
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