Increased bacterial putrescine has no impact on gut morphology and physiology in gnotobiotic adolescent mice

2013 ◽  
Vol 4 (3) ◽  
pp. 253-266 ◽  
Author(s):  
K. Slezak ◽  
L. Hanske ◽  
G. Loh ◽  
M. Blaut
Keyword(s):  
Trophic Factors ◽  
Microbial Consortia ◽  
Morphological Parameters ◽  
Gut Morphology ◽  
Germ Free ◽  
Crypt Depth ◽  
Anatomy And Physiology ◽  
Intestinal Maturation ◽  
Mucosal Growth ◽  
Fusobacterium Varium

Gut bacteria influence host anatomy and physiology. It has been proposed that bacterial metabolites including polyamines are responsible for intestinal maturation and mucosal growth. We have hypothesised that bacterially produced polyamines act as trophic factors and thereby influence large intestinal crypt depth and thickness of the different gut layers. For that purpose, germ-free mice were associated with two different microbial consortia. One group was colonised with a simplified human microbiota (SIHUMI). The second group was associated with SIHUMI + Fusobacterium varium (SIHUMI + Fv), which is known to produce high amounts of polyamines. Polyamine concentrations were measured by HPLC and morphological parameters were determined microscopically. Germ-free and conventional mice served as controls. The caecal putrescine concentration of the SIHUMI + Fv was 61.8 μM (47.6-75.5 μM), whereas that of conventional and SIHUMI mice was 28.8 μM (1.3-41.7 μM) and 24.5 μM (16.8-29.1 μM), respectively. The caecal putrescine concentration of germ-free mice was only 0.6 μM (0-1.0 μM). Caecal crypt depth and thickness of the different caecal layers revealed no significant differences between SIHUMI and SIHUMI + Fv mice. However, the crypt depth in the caeca of conventional, SIHUMI and SIHUMI + Fv mice was increased by 48.6% (P<0.001), 39.7% (P<0.001) and 28.5% (P<0.05), respectively, compared to germ-free mice. These findings indicate that increased intestinal putrescine concentrations do not influence gut morphology in our gnotobiotic adolescent mice.

scholarly journals Commensal and Opportunistic Bacteria Present in the Microbiota in Atlantic Cod (Gadus morhua) Larvae Differentially Alter the Hosts’ Innate Immune Responses

2021 ◽  
Vol 10 (1) ◽  
pp. 24
Author(s):  
Ragnhild Inderberg Vestrum ◽  
Torunn Forberg ◽  
Birgit Luef ◽  
Ingrid Bakke ◽  
Per Winge ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Responses ◽  
Atlantic Cod ◽  
Innate Immune ◽  
Rrna Gene ◽  
Innate Immune Responses ◽  
Transcriptional Responses ◽  
Gut Morphology ◽  
Germ Free ◽  
Opportunistic Bacteria

The roles of host-associated bacteria have gained attention lately, and we now recognise that the microbiota is essential in processes such as digestion, development of the immune system and gut function. In this study, Atlantic cod larvae were reared under germ-free, gnotobiotic and conventional conditions. Water and fish microbiota were characterised by 16S rRNA gene analyses. The cod larvae’s transcriptional responses to the different microbial conditions were analysed by a custom Agilent 44 k oligo microarray. Gut development was assessed by transmission electron microscopy (TEM). Water and fish microbiota differed significantly in the conventional treatment and were dominated by different fast-growing bacteria. Our study indicates that components of the innate immune system of cod larvae are downregulated by the presence of non-pathogenic bacteria, and thus may be turned on by default in the early larval stages. We see indications of decreased nutrient uptake in the absence of bacteria. The bacteria also influence the gut morphology, reflected in shorter microvilli with higher density in the conventional larvae than in the germ-free larvae. The fact that the microbiota alters innate immune responses and gut morphology demonstrates its important role in marine larval development.

Effects of spray-dried colostrum and plasmas on the performance and gut morphology of broiler chickens

2005 ◽  
Vol 56 (8) ◽  
pp. 811 ◽  
Author(s):  
M. R. King ◽  
V. Ravindran ◽  
P. C. H. Morel ◽  
D. V. Thomas ◽  
M. J. Birtles ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Broiler Chicks ◽  
Villus Height ◽  
Gut Morphology ◽  
Crypt Depth ◽  
Beneficial Effects ◽  
Bovine Plasma ◽  
Dietary Treatments ◽  
Spray Dried

The influence of feeding with starter diets containing spray-dried bovine colostrum (SBC), spray-dried bovine plasma (SBP), or spray-dried porcine plasma (SPP) on the growth performance and gut morphology of broiler chickens was investigated. The crude protein, lysine, and methionine plus cystine contents (g/kg as received) of SBC, SBP, and SPP were determined to be 766, 736, and 684; 58, 61, and 55; and 27, 28, and 24, respectively. The 3 test proteins were incorporated into a maize–soybean meal diet at a level of 50 g/kg and fed to 6 replicates of 8 male broiler chicks from 1 to 14 days of age. On Day 14, the experimental diets were replaced with a common grower-finisher diet, which was offered until Day 35. Dietary treatments had no effect (P > 0.05) on the weight gain or feed intake of birds during the first 14 days. However, feed/gain of birds fed the SBC diet was lower (P < 0.05) than of those fed the control diet. A trend for a reduction in feed/gain (P = 0.06) was observed in SBP and SPP diets. Dietary treatments had no effect (P > 0.05) on performance during Days 14–35 or Days 1–35. Villus height was increased by 5.7% (P < 0.05) and 4.6% (P = 0.05) in birds fed the SPP and SBP diets, respectively, compared with those fed the control diet. Crypt depth was 6% greater (P < 0.05) in birds fed the SPP diet and tended (P = 0.08) to be greater in birds fed the SBC diet, compared with birds fed the control. The ratio of villus height to crypt depth tended (P = 0.06) to be lower in birds offered the SBC diet compared with the control. Crypt goblet cell density was reduced (P < 0.05) by 13.9% in birds fed the SBC diet, compared with control birds. These results suggest that feeding with diets containing the SBC, SBP, and SPP during 1–14 days of age has some beneficial effects on broiler chickens. Further studies are needed to evaluate these products in less sanitary environments, which may increase the positive responses.

Stimulation of colonic mucosal growth associated with oxidized redox status in rats

2007 ◽  
Vol 292 (3) ◽  
pp. R1081-R1091 ◽  
Author(s):  
Junqiang Tian ◽  
Naohiro Washizawa ◽  
Li H. Gu ◽  
Marc S. Levin ◽  
Lihua Wang ◽  
...  
Keyword(s):  
Small Bowel ◽  
Animal Models ◽  
Redox Status ◽  
Crypt Cell ◽  
Brdu Incorporation ◽  
Intestinal Cell ◽  
Dependent Manner ◽  
Colonic Crypt ◽  
Crypt Depth ◽  
Mucosal Growth

Limited data in animal models suggest that colonic mucosa undergoes adaptive growth following massive small bowel resection (SBR). In vitro data suggest that intestinal cell growth is regulated by reactive oxygen species and redox couples [e.g., glutathione (GSH)/glutathione disulfide (GSSG) and cysteine (Cys)/cystine (CySS) redox]. We investigated the effects of SBR and alterations in redox on colonic growth indexes in rats after either small bowel transection (TX) or 80% midjejunoileal resection (RX). Rats were pair fed ± blockade of endogenous GSH synthesis with buthionine sulfoximine (BSO). Indexes of colonic growth, proliferation, and apoptosis and GSH/GSSG and Cys/CySS redox potentials (Eh) were determined. RX significantly increased colonic crypt depth, number of cells per crypt, and epithelial cell proliferation [crypt cell bromodeoxyuridine (BrdU) incorporation]. Administration of BSO markedly decreased colonic mucosal GSH, GSSG, and Cys concentrations in both TX and RX groups, with a resultant oxidation of GSH/GSSG and Cys/CySS Eh. BSO did not alter colonic crypt cell apoptosis but significantly increased all colonic mucosal growth indexes (crypt depth, cells/crypt, and BrdU incorporation) in both TX and RX groups in a time- and dose-dependent manner. BSO significantly decreased plasma GSH and GSSG, oxidized GSH/GSSG Eh, and increased plasma Cys and CySS concentrations. Collectively, these data provide in vivo evidence indicating that oxidized colonic mucosal redox status stimulates colonic mucosal growth in rats. The data also suggest that GSH is required to maintain normal colonic and plasma Cys/CySS homeostasis in these animal models.

scholarly journals EFFECT OF PEPPERMINT (MENTHA PIPERITA) POWDER ON PERFORMANCE, GUT MORPHOLOGY AND IMMUNE ORGANS RESPONS OF COCCIDIOSIS INFECTED BROILERS

2021 ◽  
Vol 52 (2) ◽  
pp. 276-290
Author(s):  
S. M. Hussein
Keyword(s):  
Weight Gain ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Additive ◽  
Mentha Piperita ◽  
Feed Conversion ◽  
Villus Height ◽  
Gut Morphology ◽  
Crypt Depth ◽  
Immune Organs

This experiment was performed to find out the effect of peppermint on the performance, gut morphology and immune organs of coccidiosis challenged broilers. A feeding trail was conducted on 320-day-old Ross 308 chicks allocated to 32 floor pens in a 2x4 factorial arrangement. The studied factors were: challenge (Negative or Positive) and feed additive (Control, Peppermint and). On day 8 of age, birds in challenged groups were orally inoculated with Eimeria tenella. On day 35 Eimeria decreased weight (P <.0001) gain and deteriorated feed conversion ratio (P < 0.05). On 24 and 35 days of age, broilers given salinomycin and peppermint had improved weight gain and feed conversion ratio compared to controls (P < 0.05). Significant challenge × additive interactions were observed for weight gain, feed intake, feed conversion ratio villus height, crypt depth and apparent villus surface area on d 24 and d 35. Challenged broilers in salinomycin and peppermint groups had significantly increased villus height and villus height/crypt depth and decreased crypt depth compared to positive control. Challenged birds exhibited significantly longer, wider and larger area of bursa of Fabricius lymphoid follicles compared to non-challenged birds. These results illustrated that, peppermint has high protective efficacy against declining in the performance and gut health of coccidiosis infected broilers.

scholarly journals Effects of Thermally Oxidized Vegetable Oil on Growth Performance and Carcass Characteristics, Gut Morphology, Nutrients Utilization, Serum Cholesterol and Meat Fatty Acid Profile in Broilers

Catalysts ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1528
Author(s):  
Ghulam Yaseen ◽  
Muhammad A. Sarfraz ◽  
Saima Naveed ◽  
Farooq Ahmad ◽  
Fehmeada Bibi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Growth Performance ◽  
Fatty Acid Profile ◽  
Vegetable Oil ◽  
Serum Cholesterol ◽  
Carcass Weight ◽  
Villus Height ◽  
Gut Morphology ◽  
Crypt Depth

The impacts of dietary levels of oxidized vegetable (sunflower) oil on growth performance, gut morphology, nutrients utilization, serum cholesterol and meat fatty acid profile were evaluated in Ross 308 straight-run (n = 192) day-old broilers. The broilers were arbitrarily distributed among four dietary treatments including; FVO: fresh vegetable oil (1 mEq kg−1), LOO: low oxidized (20 mEq kg−1), MOO: moderately oxidized (40 mEq kg−1), and HOO: highly oxidized vegetable oil (60 mEq kg−1) with 5% inclusion containing six replicates. Results revealed that the broilers consuming MOO and HOO based diets showed reduced (p = 0.05) feed intake, body weight gain and carcass weight accompanied by a poorer feed conversion ratio than those consuming FVO. Villus height, villus height to crypt depth ratio, ileal digestibility of crude protein (p = 0.041), crude fat (p = 0.032) and poly unsaturated fatty acids (p = 0.001) in thigh muscles were decreased, whereas crypt depth (p = 0.001), serum cholesterol levels (p = 0.023) and short chain fatty acids (p = 0.001) were increased (p < 0.001) by increasing dietary oxidation level. In conclusion, MOO and HOO exerted deleterious effects on growth, carcass weight, gut development and nutrients utilization. Low oxidized vegetable oil (20 mEq kg−1), however, with minimum negative effects can be used as a cost effective energy source in poultry diets.

scholarly journals In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut

2007 ◽  
Vol 104 (18) ◽  
pp. 7622-7627 ◽  
Author(s):  
John F. Rawls ◽  
Michael A. Mahowald ◽  
Andrew L. Goodman ◽  
Chad M. Trent ◽  
Jeffrey I. Gordon
Keyword(s):  
Immune Responses ◽  
Innate Immune ◽  
Microbial Interactions ◽  
Microbial Consortia ◽  
Innate Immune Responses ◽  
Germ Free ◽  
Evolutionarily Conserved ◽  
Bacterial Genes ◽  
The Impact

Complex microbial communities reside within the intestines of humans and other vertebrates. Remarkably little is known about how these microbial consortia are established in various locations within the gut, how members of these consortia behave within their dynamic ecosystems, or what microbial factors mediate mutually beneficial host–microbial interactions. Using a gnotobiotic zebrafish–Pseudomonas aeruginosa model, we show that the transparency of this vertebrate species, coupled with methods for raising these animals under germ-free conditions can be used to monitor microbial movement and localization within the intestine in vivo and in real time. Germ-free zebrafish colonized with isogenic P. aeruginosa strains containing deletions of genes related to motility and pathogenesis revealed that loss of flagellar function results in attenuation of evolutionarily conserved host innate immune responses but not conserved nutrient responses. These results demonstrate the utility of gnotobiotic zebrafish in defining the behavior and localization of bacteria within the living vertebrate gut, identifying bacterial genes that affect these processes, and assessing the impact of these genes on host–microbial interactions.

scholarly journals Desacetyl-α-MSH and α-MSH have sex specific interactions with diet to influence mouse gut morphology, metabolites and microbiota

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo Sun ◽  
Tommi Vatanen ◽  
Thilini N. Jayasinghe ◽  
Elizabeth McKenzie ◽  
Rinki Murphy ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Specific Interactions ◽  
Microbiota Composition ◽  
High Fat ◽  
Appetite Control ◽  
Gut Morphology ◽  
Crypt Depth ◽  
Melanocortin Peptides ◽  
Gut Microbiota Composition

Abstract The melanocortin peptides have an important role in regulating body weight and appetite. Mice that lack the desacetyl-α-MSH and α-MSH peptides (Pomctm1/tm1) develop obesity. This effect is exacerbated by a high fat diet (HFD). However, development of obesity in female Pomctm1/tm1 mice during chronic HFD conditions is not fully accounted for by the increased energy intake. We hypothesized that the protection against chronic HFD-induced obesity imparted by MSH peptides in females is mediated by sex-specific alterations in the gut structure and gut microbiota. We determined that female WT mice had reduced jejunum villus length and increased crypt depth in response to chronic HFD. WT males and Pomctm1/tm1 mice lacked this adaptation to a chronic HFD. Both Pomctm1/tm1 genotype and chronic HFD were significantly associated with gut microbiota composition. Sex-specific associations between Pomctm1/tm1 genotype and gut microbiota were observed in the presence of a chronic HFD. Pomctm1/tm1 females had significantly reduced fecal acetate and propionate concentrations when compared to WT females. We conclude that MSH peptides influence jejunum villus length, crypt depth and the structure of the gut microbiota. These effects favor reduced nutrient absorption and occur in addition to the recognized roles of desacetyl-α-MSH and α-MSH peptides in appetite control.

scholarly journals Computer-guided design of optimal microbial consortia for immune system modulation

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Richard R Stein ◽  
Takeshi Tanoue ◽  
Rose L Szabady ◽  
Shakti K Bhattarai ◽  
Bernat Olle ◽  
...  
Keyword(s):  
Immune System ◽  
Gut Microbiota ◽  
Experimental Validation ◽  
Ecological Model ◽  
Microbial Consortia ◽  
Great Promise ◽  
Germ Free ◽  
Systematic Selection ◽  
Immune System Modulation ◽  
Selection Of

Manipulation of the gut microbiota holds great promise for the treatment of diseases. However, a major challenge is the identification of therapeutically potent microbial consortia that colonize the host effectively while maximizing immunologic outcome. Here, we propose a novel workflow to select optimal immune-inducing consortia from microbiome compositicon and immune effectors measurements. Using published and newly generated microbial and regulatory T-cell (Treg) data from germ-free mice, we estimate the contributions of twelve Clostridia strains with known immune-modulating effect to Treg induction. Combining this with a longitudinal data-constrained ecological model, we predict the ability of every attainable and ecologically stable subconsortium in promoting Treg activation and rank them by the Treg Induction Score (TrIS). Experimental validation of selected consortia indicates a strong and statistically significant correlation between predicted TrIS and measured Treg. We argue that computational indexes, such as the TrIS, are valuable tools for the systematic selection of immune-modulating bacteriotherapeutics.

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Five Decades of Research Experience in Speech Anatomy and Physiology

2016 ◽  
Vol 1 (5) ◽  
pp. 4-12
Author(s):  
David P. Kuehn
Keyword(s):  
Ohio State University ◽  
State University ◽  
Research Experience ◽  
University Of Iowa ◽  
University Of Illinois ◽  
Anatomy And Physiology ◽  
The Ohio State University ◽  
Speech Science ◽  
The University ◽  
East Carolina University

This report highlights some of the major developments in the area of speech anatomy and physiology drawing from the author's own research experience during his years at the University of Iowa and the University of Illinois. He has benefited greatly from mentors including Professors James Curtis, Kenneth Moll, and Hughlett Morris at the University of Iowa and Professor Paul Lauterbur at the University of Illinois. Many colleagues have contributed to the author's work, especially Professors Jerald Moon at the University of Iowa, Bradley Sutton at the University of Illinois, Jamie Perry at East Carolina University, and Youkyung Bae at the Ohio State University. The strength of these researchers and their students bodes well for future advances in knowledge in this important area of speech science.

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