Analysis of the human intestinal microbiota from 92 volunteers after ingestion of identical meals

2013 ◽  
Vol 4 (2) ◽  
pp. 187-193 ◽  
Author(s):  
J.S. Jin ◽  
M. Touyama ◽  
R. Kibe ◽  
Y. Tanaka ◽  
Y. Benno ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Restriction Fragment Length Polymorphism ◽  
Intestinal Microbiota ◽  
Fragment Length Polymorphism ◽  
Microbiota Composition ◽  
Key Factors ◽  
Enzyme Systems ◽  
Human Intestinal Microbiota ◽  
Faecal Samples ◽  
Restriction Fragment Length

The intestinal microbiota composition of 92 volunteers living in Japan was identified following the consumption of ‘identical meals’ (1,879 kcal/day) for 3 days. When faecal samples were analysed by terminal restriction fragment length polymorphism with several primer-restriction enzyme systems and then clustered, the patterns could be divided into 2 clusters. Contribution tests and partition modelling showed that OTU211 of the 35f-MspI system and OTU237 of the 35f-AluI system were key factors in the distribution of these groups. However, significant differences among these groups in terms of body mass index and age were not observed.

Significant Relationships of Plasma Lipids and Body Mass Index with Polymorphisms at the Linked Low-Density-Lipoprotein Receptor Gene and Insulin Receptor Gene Loci (19P13.2) in Essential Hypertensive Patients

1994 ◽  
Vol 86 (5) ◽  
pp. 583-592 ◽  
Author(s):  
Brian J. Morris ◽  
Robert Y. L. Zee ◽  
Bruce G. Robinson
Keyword(s):  
Body Mass Index ◽  
Restriction Fragment Length Polymorphism ◽  
Body Mass ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Density Lipoprotein ◽  
Hypertensive Patients ◽  
Restriction Fragment Length

1. Recent molecular genetic studies have implicated the low-density-lipoprotein receptor gene locus (LDLR, at chromosome 19p13.2) in obesity in essential hypertensive patients and in the atherogenic lipoprotein phenotype. The present study examined genotypes for the obesity-associated ApaLI restriction fragment length polymorphism of LDLR, and genotypes for a hypertension-associated RsaI restriction fragment length polymorphism at the insulin receptor gene (INSR) locus, which is linked to LDLR, in relation to plasma lipids, body mass index and blood pressure in 27 obese and 57 non-obese Caucasians with severe essential hypertension, selected on the basis of having parents who were both hypertensive, and in 25 obese and 45 non-obese normotensive subjects selected on the basis of having parents who were both normotensive after the age of 50 years. 2. Plasma triacylglycerol and low-density-lipoprotein-cholesterol were elevated in hypertensive patients, but did not differ between the obese and non-obese hypertensive groups. Significant positive correlations were seen between body mass index and triacylglycerol and low-density-lipoprotein-cholesterol in the obese and non-obese hypertensive patients, respectively. In addition, obese hypertensive patients had significantly higher diastolic blood pressure than non-obese hypertensive patients. 3. The eight obese hypertensive patients who were homozygous for the obesity-associated 6.6 kb allele of the ApaLI restriction fragment length polymorphism of LDLR (‘6.6.kb homozygotes’) had a significantly higher body mass index [34 ± 6.0 (SD) kg/m2] than the 18 heterozygotes (29 ± 2.7 kg/m2) and the single subject who was homozygous for the 9.4 kb allele (29 kg/m2) (P = 0.012 by one-way analysis of variance). The body mass index of the eight hypertensive 6.6 kb homozygotes was also greater than the body mass index of 29 ± 2.4 kg/m2 observed for the eight obese normotensive 6.6 kb homozygotes. In addition, the eight obese hypertensive 6.6 kb homozygotes had a higher plasma triacylglycerol [4.2 ± 0.77 (SEM) mmol/l] than the 18 obese hypertensive heterozygotes (2.4 ± 0.33 mmol/l; P = 0.045). Non-obese hypertensive patients showed no significant gentotypic differences in relation to the LDLR restriction fragment length polymorphism. 4. In the normotensive group, however, the frequency of the 6.6 kb allele of the LDLR ApaLI restriction fragment length polymorphism in obese subjects (0.54) was not significantly greater than in non-obese subjects (0.48) [cf. the significantly (P = 0.004) different values of 0.63 and 0.39, respectively, in obese and non-obese hypertensive patients]. Moreover, the LDLR genotype showed no significant relationship to the body mass index or plasma lipids in the obese and non-obese normotensive groups. 5. In the case of genotypes for the INSR restriction fragment length polymorphism, the only difference was slightly higher total and low-density-lipoprotein-cholesterol in non-obese hypertensive patients who possessed the hypertension-associated 7.0 kb allele. 6. Comparison of combined genotypes for LDLR and INSR restriction fragment length polymorphisms in hypertensive patients indicated that each associated independently in the various groups. 7. In conclusion, the present study has found that in obese hypertensive patients a LDLR variant is associated with higher body mass index and plasma triacylglycerol, although a causal relationship of LDLR in obesity and hyperlipidaemia in such patients remains to be established.

scholarly journals Effects on Microbiota Composition after Consumption of Quinoa Beverage Fermented by a Novel Xylose-Metabolizing L. plantarum Strain

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3318
Author(s):  
Pamela Canaviri-Paz ◽  
Elin Oscarsson ◽  
Anna Kjellström ◽  
Hanna Olsson ◽  
Chandana Jois ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Starter Culture ◽  
Probiotic Strain ◽  
Well Being ◽  
Microbiota Composition ◽  
Probiotic Potential ◽  
Faecal Samples ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Restriction Fragment Length

Demands for novel lactic acid bacteria with potential to be used as probiotics along with healthy fermented plant-based products increase worldwide. In this study, a novel Lactiplantibacillus plantarum P31891 strain with enzymatic capacity to degrade tannins and ferment xylose was used as starter culture for fermentation of a quinoa-based beverage. The probiotic potential of the selected strain was evaluated in healthy volunteers. Twenty participants consumed the beverage for 14 days; microbiota changes in saliva and faecal samples were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP), Next Generation Sequencing (NGS) and qPCR; and gastrointestinal well-being and digestive symptoms were recorded. The results indicated that the consumption of the beverage with Lactiplantibacillus plantarum P31891 in a probiotic dose (1012 CFU/mL) increased the number of Lactobacillus in the feces but not in saliva. Overall, the bacterial community did not seem to be influenced by the bacterium or by the beverage, as expressed by the diversity indexes, but specific genera were affected, as reflected in changes in amplicon sequence variants. Consequently, Lactiplantibacillus plantarum P31891 showed potential to be categorized as a probiotic strain in the fermented quinoa-based beverage.

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