scholarly journals Molecular characterization of genetic variability and structure of olive(Olea europaea L.) germplasm collection analyzed by agromorphologicaltraits and microsatellite markers

2016 ◽  
Vol 40 ◽  
pp. 583-596 ◽  
Author(s):  
Karim SORKHEH ◽  
Esmaeil KHALEGHI
2014 ◽  
Vol 12 (3) ◽  
pp. 273-277 ◽  
Author(s):  
Aliki Xanthopoulou ◽  
Ioannis Ganopoulos ◽  
Georgios Koubouris ◽  
Athanasios Tsaftaris ◽  
Chrysa Sergendani ◽  
...  

Olea europaea L. has been cultivated in the Mediterranean region for thousands of years and is of major economic importance. The origin of olive cultivars remains as complex to trace as their identification. Thus, their molecular characterization and discrimination will enable olive germplasm management. In addition, it would be a useful tool for authentication of olive products. High-resolution melting (HRM) analysis, coupled with five microsatellite markers, was integrated to facilitate molecular identification and characterization of main O. europaea cultivars collected from the National Olive Tree Germplasm Collection established in Chania, Greece. The five microsatellite loci used were highly informative and generated a unique melting curve profile for each of the 47 cultivars and for each microsatellite tested. In particular, three microsatellite markers (DCA03, DCA09 and DCA17), which generated 29 HRM profiles, were sufficient to genotype all the olive cultivars studied, highlighting their potential use for cultivar identification. Furthermore, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited for molecular characterization of olive cultivars.


2009 ◽  
Vol 66 (5) ◽  
pp. 685-690 ◽  
Author(s):  
Innocenzo Muzzalupo ◽  
Francesca Stefanizzi ◽  
Amelia Salimonti ◽  
Rosanna Falabella ◽  
Enzo Perri

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.


2014 ◽  
Vol 10 (4) ◽  
pp. 895-909 ◽  
Author(s):  
Nicolò G. M. Cultrera ◽  
Fiammetta Alagna ◽  
Roberto Mariotti ◽  
Francesca De Marchis ◽  
Andrea Pompa ◽  
...  

2014 ◽  
Vol 57 ◽  
pp. 15-19 ◽  
Author(s):  
Giuseppina Las Casas ◽  
Francesco Scollo ◽  
Gaetano Distefano ◽  
Alberto Continella ◽  
Alessandra Gentile ◽  
...  

2011 ◽  
Vol 133 (1) ◽  
pp. 247-259 ◽  
Author(s):  
Maria do Rosário Félix ◽  
Carla M. Reis Varanda ◽  
Maria Ivone E. Clara

2011 ◽  
Vol 11 (1) ◽  
pp. 122 ◽  
Author(s):  
Krzysztof Zienkiewicz ◽  
Agnieszka Zienkiewicz ◽  
María Rodríguez-García ◽  
Antonio J Castro

2011 ◽  
Vol 151 (2) ◽  
pp. 204-214 ◽  
Author(s):  
Inian Moorthy ◽  
John R. Miller ◽  
Jose Antonio Jimenez Berni ◽  
Pablo Zarco-Tejada ◽  
Baoxin Hu ◽  
...  

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