scholarly journals Toward Construction of a Brain in a Dish: Cultured Neurons, Brain Organoids, and Beyond

2021 ◽  
Vol 28 (4) ◽  
pp. 151-161
Author(s):  
Yoshiho Ikeuchi
Keyword(s):  
Cultured Neurons

β-Amyloid Upregulates Intracellular Clusterin but not Secretory Clusterin in Primary Cultured Neurons and APP Mice

2017 ◽  
Vol 14 (11) ◽  
Author(s):  
Ping Wang ◽  
Keliang Chen ◽  
Yuehua Gu ◽  
Qihao Guo ◽  
Zhen Hong ◽  
...  
Keyword(s):  
Cultured Neurons ◽  
Β Amyloid ◽  
Primary Cultured Neurons

Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (10) ◽  
Author(s):  
Margarida Caldeira ◽  
Joana Ferreira ◽  
Ana Carvalho ◽  
Carlos Duarte
Keyword(s):  
Plasma Membrane ◽  
Cultured Neurons ◽  
Associated Proteins

scholarly journals The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 262
Author(s):  
Qin-Wei Wu ◽  
Josef P. Kapfhammer
Keyword(s):  
Rna Editing ◽  
Cortical Neurons ◽  
High Efficiency ◽  
Primary Cultures ◽  
Cultured Neurons ◽  
Therapeutic Tool ◽  
Bacterial Enzyme ◽  
Rna Targeting ◽  
Cultured Cortical Neurons ◽  
New Generation

The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is currently being developed as a new therapeutic tool for the treatment of neurological and other diseases. However, the safety of this new generation of RNA therapies is still unclear. In this study, we constructed a vector expressing CRISPR-Cas13 under a constitutive neuron-specific promoter. CRISPR-Cas13 from Leptotrichia wadei was expressed in primary cultures of mouse cortical neurons. We found that the presence of CRISPR-Cas13 impedes the development of cultured neurons. These results show a neurotoxic action of Cas13 and call for more studies to test for and possibly mitigate the toxic effects of Cas13 enzymes in order to improve CRISPR-Cas13-based tools for RNA targeting.

scholarly journals Vasopressin Expression in Cultured Neurons is Stimulated by Cyclic AMP

1990 ◽  
Vol 2 (6) ◽  
pp. 859-865 ◽  
Author(s):  
Petra Ceding ◽  
Karl Schilling ◽  
Hartwig Schmale
Keyword(s):  
Cyclic Amp ◽  
Cultured Neurons

P1-198: VISUALIZING THE INTERACTION OF FLUORESCENTLY TAGGED AMYLOID BETA WITH CULTURED NEURONS

2006 ◽  
Vol 14 (7S_Part_6) ◽  
pp. P354-P355
Author(s):  
Mirela Mustata ◽  
Zoe Proulx ◽  
Jenna Guglielmo ◽  
Sarah R. Gallant
Keyword(s):  
Amyloid Beta ◽  
Cultured Neurons
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