scholarly journals DNA barcoding of rhopalosomatid larvae reveals a new host record and genetic evidence of a second species of Rhopalosoma Cresson (Hymenoptera, Rhopalosomatidae) in America north of Mexico

2019 ◽  
Vol 74 ◽  
pp. 35-46 ◽  
Author(s):  
Lance A. Miller ◽  
Torie D. Benefield ◽  
Sarah A. Lounsbury ◽  
Volker Lohrmann ◽  
Jeremy D. Blaschke

Rhopalosomatidae are unusual wasps whose larvae develop as ectoparasitoids on crickets. In America north of Mexico, three genera and six species are recognized. Host species are known only for Rhopalosoma nearcticum Brues and include Hapithus agitator Uhler, H. brevipennis (Saussure), and H. saltator (Uhler) (Gryllidae: Hapithinae). Here we report a new host species: the Anaxipha exigua (Say) species-group (Trigonidiidae: Trigonidiinae) discovered by barcoding nine rhopalosomatid larvae collected from Cypress Grove Nature Park, Jackson, Tennessee. Rhopalosoma nearcticum is currently the only documented species of Rhopalosoma Cresson in America north of Mexico, but our phylogenetic analyses recovered two genetically distinct clades of Rhopalosoma and thus reveal the presence of at least two species of Rhopalosoma in America north of Mexico.

Check List ◽  
2017 ◽  
Vol 13 (1) ◽  
pp. 2029 ◽  
Author(s):  
Ace Kevin S. Amarga ◽  
Philip A. Alviola ◽  
Ireneo L. Lit, Jr. ◽  
Sheryl A. Yap

This paper constitutes the first ectoparasite faunal survey of bats for Marinduque Island, Philippines. From 1–12 June 2010, 150 bats belonging to 11 species were captured in 11 caves on the island. Each bat was sampled for ectoparasitic arthropods, and a total of 587 individuals representing 21 species, belonging to five families (Acari: Argasidae and Spinturnicidae; Diptera: Nycteribiidae and Streblidae; and Siphonaptera: Ischnopsyllidae) were collected. New host records (new host record) in the Philippines for Brachytarsina cucullata Jobling 1934, B. proxima Jobling 1951, B. werneri Jobling 1951, Raymondia pseudopagodarum Jobling 1951, Eucampsipoda philippinensis Ferris 1924, Nycteribia allotopa Speiser 1901, Nycteribia allotopoides Theodor 1963, Nycteribia parvuloides Theodor 1963, Ancystropus taprobanius (Turk 1950), and Carios batuensis Hirst 1929 were documented. A checklist of the ectoparasitic species known from the Philippines, their distribution, and bat host species is provided.


Zootaxa ◽  
2007 ◽  
Vol 1450 (1) ◽  
pp. 57-62
Author(s):  
LUIZ C.C. DAUDT ◽  
JOSÉ F.R. AMATO

Stratiodrilus circensis Steiner & Amaral, 1999, ectocommensal of anomuran crabs Aegla leptodactyla Buckup & Rossi, 1977 (New Host Record), is first recorded for the State of Rio Grande do Sul, southern Brazil, with some considerations on the observed morphological variation and the identification of its type host species. Thirty crabs were examined, 70% of which were positive for S. circensis. Intensity of infestation varied from 1 to 41 (arithmetic mean 13.3; SD 10.4). Eleven specimens of S. circensis from Aegla schmitti Hobbs III, 1979, collected from Rio Carvalho, tributary of Rio Piraquara, State of Paraná, (the type locality), were also examined. Differences in total body length, length of antennae, cirri, conical ventral lobes, and tubercles were found between the specimens described here, the specimens described in previous works, and the specimens from A. schmitti from Rio Carvalho. The geographical distribution of S. circensis is discussed


Zootaxa ◽  
2013 ◽  
Vol 3608 (3) ◽  
pp. 204-214 ◽  
Author(s):  
SCOTT R. SHAW ◽  
PAUL M. MARSH ◽  
MIRANDA A. TALLUTO

The Aleiodes pallidator species-group is defined, and an identification key is provided for the five species known to occur in the U.S.A. and Canada. Two new species are described: Aleiodes martini Shaw and Marsh, from Florida, and A. xanthoclypeus Shaw and Marsh, known from Canada and Wisconsin, and reared from Lymantriidae species including Dasychira plagiata (Walker) and Olene grisefacta (a new host record for the genus Aleiodes). Five species are illustrated, and their host associations are summarized.


Zootaxa ◽  
2018 ◽  
Vol 4521 (4) ◽  
pp. 593 ◽  
Author(s):  
JOHN SMIT ◽  
IVO RAEMAKERS ◽  
KEVIN BEENTJES

Thick-headed flies of the genus Myopa Fabricius are a common sight in early spring in Europe. Several species can be found flying among Salix catkins together with their supposed Andrena Fabricius hosts (Hymenoptera: Apidae) (John Smit observations). Despite the fact that some of these Myopa species are very common, little is known about their actual host-parasitoid associations. Only a few scattered host records can be found in the literature, most of which refer to M. testacea s.l.. Whether or not these records actually concern M. testacea (Linnaeus) is uncertain due to the confusion over the identity of several species within the M. testacea species group (Stuke & Clements 2008). Stuke (2017), in his World Catalogue of Conopids, provides all recorded host associations and distinguishes between unambiguously accepted host records and doubtful records based on three criteria: 1) the conopid has been reared from the host or a larva has been identified by DNA barcoding; 2) there is no doubt concerning the identity of the conopid species concerned, and 3) there is no doubt concerning the identity of the host species. Because the vast majority of the host records in the literature do not fulfil one or more of these criteria they are regarded as doubtful (Stuke 2017). Thus, only three host associations for the genus Myopa can be regarded as confirmed: Andrena vaga Panzer, for both M. hirsuta Stuke & Clements (Jentzsch 2009) and M. testacea (Erteld 1998, Fellendorf et al. 2004, De Meijere 1912), and the Nearctic Andrena regularis Malloch, for the Holarctic M. vicaria Walker (Miliczky & Osgood 1995). Here we report on a fourth host association for the genus Myopa, since a second instar larva of M. pellucida Robineau-Desvoidy was found in the abdomen of an Andrena nitida (Müller) female and identified using DNA barcoding. 


MycoKeys ◽  
2020 ◽  
Vol 70 ◽  
pp. 59-88
Author(s):  
Danushka S. Tennakoon ◽  
Kasun M. Thambugala ◽  
Dhanushka N. Wanasinghe ◽  
Eleni Gentekaki ◽  
Itthayakorn Promputtha ◽  
...  

A novel ascomycetous genus, Elongaticollum, occurring on leaf litter of Hedychium coronarium (Zingiberaceae) in Taiwan, is described and illustrated. Elongaticollum is characterized by dark brown to black, superficial, obpyriform, pycnidial conidiomata with a distinct elongate neck, and oval to oblong, hyaline, aseptate conidia. Phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian) of combined ITS, LSU, SSU and tef1-α sequence data revealed Elongaticollum as a distinct genus within the family Phaeosphaeriaceae with high statistical support. In addition, Ophiosphaerella taiwanensis and Phaeosphaeriopsis beaucarneae are described as new species from dead leaves of Agave tequilana and Beaucarnea recurvata (Asparagaceae), respectively. Neosetophoma poaceicola is reported as a new host record from dead leaves of Musa acuminata (Musaceae). Newly described taxa are compared with other similar species and comprehensive descriptions and micrographs are provided.


MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 91-109 ◽  
Author(s):  
Danushka S. Tennakoon ◽  
Kasun M. Thambugala ◽  
Rajesh Jeewon ◽  
Sinang Hongsanan ◽  
Chang-Hsin Kuo ◽  
...  

A novel ascomycete genus, Longihyalospora, occurring on leaf litter of Ficus ampelas in Dahu Forest Area in Chiayi, Taiwan is described and illustrated. Longihyalospora is characterized by dark mycelium covering the upper leaf surface, elongate mycelial pellicle with ring of setae, pale brown to brown peridium, broadly obovoid, short pedicellate asci and hyaline, fusiform, elongated (tapering ends) and multi-septate ascospores with a thin mucilaginous sheath. Phylogenetic analyses of combined ITS, LSU and SSU sequence data revealed Longihyalospora as a distinct genus within the Chaetothyriaceae with high bootstrap support. Moreover, based on morphological similarities, Chaetothyrium vermisporum transferred to the new genus. In addition, Ceramothyrium longivolcaniforme is reported for the first time on Ficus ampelas. Newly added species are compared with other similar species and comprehensive descriptions and micrographs are provided.


Author(s):  
A.K. Verma ◽  
J. Verma

Background: Heteraxinoides atlanticus Gayevskaya et Kovaleva (1979) is described from the locality of Arabian Sea, India. This monogenean species is first time reported from India, infesting the gills of Nemipterus japonicus (Bloch). The present study redescribes H. atlanticus and its phylogenetic status using morphometric and molecular tools. Methods: A total of 35 parasites were collected from the 234 specimens of Nemipterus japonicus at Mumbai, India. The temporary and permanent mounts were prepared for the morphometric analyses of H. atlanticus. For PCR, the genomic DNA was isolated from the parasites using primers for 18S rDNA, 28S rDNA and mtCOI gene. The obtained DNA sequences were subjected to different bioinformatics softwares (BLASTn, Clustal Omega and MEGA) for phlyogenetic analyses.Result: Phylogenetic analyses with the help of partial 18S and 28S rDNA sequences of H. atlanticus and other available sequences of heteraxinids provided the better understanding of relationship in the family Heteraxinidae. Another species, H. karachiensis Hadi and Bilqees (2014) showed similar morphological features such as number of clamps and structure of genital atrium to H. atlanticus except few minor differences, so it must be considered as synonym of H. atlanticus and placed into Heteraxinidae instead of Axinidae.


MycoKeys ◽  
2021 ◽  
Vol 79 ◽  
pp. 173-192
Author(s):  
Digvijayini Bundhun ◽  
Rajesh Jeewon ◽  
Indunil C. Senanayake ◽  
Erio Camporesi ◽  
Janith V. S. Aluthmuhandiram ◽  
...  

Collections of fungal samples from two dead leaf specimens from Italy were subjected to morphological examination and phylogenetic analyses. Two coelomycetous taxa belonging to two different genera in Xylariomycetidae, Sordariomycetes, namely Discosia and Sporocadus, were identified. The Discosia taxon is revealed as a new species and is herein introduced as Discosia ravennicasp. nov. while the Sporocadus taxon is identified as Sporocadus rosigena. Multi-locus phylogeny based on DNA sequence data of the large subunit (LSU) and internal transcribed spacer (ITS) of nuclear ribosomal genes, β-tubulin (β-tub) and RNA polymerase II second largest subunit (rpb2) showed that D. ravennica is related to D. neofraxinea but it forms an independent lineage that supports its new species status. The new taxon also differs from other Discosia species by its unilocular to bilocular, superficial and applanate conidiomata with basal stroma composed of cells of textura angularis, elongate-ampulliform conidiogenous cells and conidia smaller in size. Sporocadus rosigena is here reported as a new host record from Quercus ilex from Italy. Descriptions, illustrations and molecular data for both species are provided in this paper.


MycoKeys ◽  
2019 ◽  
Vol 60 ◽  
pp. 69-92 ◽  
Author(s):  
De-Ping Wei ◽  
Dhanushka N. Wanasinghe ◽  
Kevin D. Hyde ◽  
Peter E. Mortimer ◽  
Jianchu Xu ◽  
...  

Simplicillium species have a wide host range and an extensive distribution. Some species are associated with rusts, as well as other plant pathogenic fungi and play an important role in biological control. In this study, two specimens of Simplicillium were collected from Chiang Mai Province, Thailand. Simplicillium formicae sp. nov. was isolated from an infected ant and S. lanosoniveum from Ophiocordyceps unilateralis which is a new host record. Species were initially identified using ITS gene sequences and confirmed using morphology coupled with phylogenetic analyses of a combined nrLSU, nrSSU, TEF and RPB1 dataset. Simplicillium formicae differs from other species in the genus by the presence of flask-shaped synnemata and phialides with intercalary nodes. Simplicillium lanosoniveum resembles other collections of the species by its completely solitary, tapering phialides and globose to ellipsoidal conidia which adhere in a slimly head. A key to species of Simplicillium is also provided.


2010 ◽  
Vol 55 (3) ◽  
Author(s):  
María Rosario Robles ◽  
James Loudon ◽  
John Kinsella ◽  
Mandala Hunter-Ishikawa ◽  
David Miller ◽  
...  

AbstractLemuricola (Madoxyuris) bauchoti Chabaud, Brygoo et Petter, 1965 is redescribed from material collected from the ring-tailed lemur, Lemur catta, from the Beza Mahafaly Special Reserve in Madagascar using the scanning electron microscope. This is a new host record and the first oxyurid reported from the ring-tailed lemur. Previously, records of each species of the subgenus Madoxyuris have been restricted to a single host species, but the close relationship between these nematodes and their Strepsirrhini hosts will only be proven when additional records fill in the gaps in their distribution.


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