scholarly journals Geometric morphometric discrimination of the three African honeybee subspecies Apis mellifera intermissa, A. m. sahariensis and A. m. capensis (Hymenoptera, Apidae): Fore wing and hind wing landmark configurations

2016 ◽  
Vol 52 ◽  
pp. 61-70 ◽  
Author(s):  
Choukri Barour ◽  
Michel Baylac
Keyword(s):  
Apis Mellifera ◽  
Fore Wing ◽  
Hind Wing ◽  
Geometric Morphometric ◽  
Apis Mellifera Intermissa ◽  
Honeybee Subspecies ◽  
African Honeybee

scholarly journals IDENTIFICATION OF THE HONEYBEE SUBSPECIES FROM SOME ROMANIAN COUNTIES USING A SEMIAUTOMATIC SYSTEM FOR ANALYZING WINGS

2018 ◽  
Vol 6 ◽  
pp. 1124-1128
Author(s):  
Elena Buescu ◽  
Maria Rodica Gurau ◽  
Doina Danes
Keyword(s):  
Apis Mellifera ◽  
Morphometric Analysis ◽  
Geometric Morphometric ◽  
Eastern Area ◽  
Three Samples ◽  
Morphometric Methods ◽  
Semiautomatic System ◽  
Geometric Morphometric Analysis ◽  
Honeybee Subspecies ◽  
Geometric Morphometric Method

Morphometric analysis of honeybee wings can be used to discriminate between honeybee subspecies. The classic morphometric methods used to identify honeybee subspecies are time consuming while geometric morphometric analysis are proven to be more efficient and rapid for the identification of honeybee subspecies.  Even if the geometric morphometric analysis involves several steps such as: obtaining the image of the honeybee wing,  processing the wings image (settlement of the 19 points), classification and validation, in terms of consumed time,it  takes less than classic morphometric methodes and it is easier to apply. The aim of this study was to identify honeybee subspecies from the Romanian South-Eastern area, using the geometric morphometric method. To accomplish this, the semiautomated French system ApiClass was used. This system  allows us to identify the honeybee subspecies based on the wing's image of the Apis mellifera honeybee worker. The program is using the recent approaches of geometric morphometry and is analyzing the wing considering 19 points  corresponding to the intersections of the main veins of the bee forewings. These coordinates are processed by the system before being analyzed  after which the system returns the probability of  the analyzed honeybee belonging to one of the honeybee subspecies from its reference system. The system has more than 5000 honeybee wing references  off the main lines and honeybee subspecies.  Using this system we analyzed  samples from the counties of Braila, Buzau, Galati and Ilfov. Each sample was composed from wings of  20 honeybees. Most of the analyzed samples were identified as hybrids without being possible  to specify the type or the level of the hybridization: only three samples were identified as being Apis mellifera carnica presenting a homology degree over 90%.

scholarly journals Procruste analysis of forewing shape in two endemic honeybee subspecies Apis mellifera intermissa and A. m. sahariensis from the Northwest of Algeria

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Fouzia Abed ◽  
Benabdellah Bachir-Bouiadjra ◽  
Lahouari Dahloum ◽  
Abdulmojeed Yakubu ◽  
Ahmed Haddad ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Geometric Approach ◽  
Wing Shape ◽  
Shape Variation ◽  
Apis Mellifera Intermissa ◽  
Significant Difference ◽  
Validation Procedure ◽  
Honeybee Subspecies ◽  
The Right

Abstract. Abed F, Bachir-Bouiadjra B, Dahloum L, Yakubu A, Haddad A, Homrani A. 2021. Procruste analysis of forewing shape in two endemic honeybee subspecies Apis mellifera intermissa and A. m. sahariensis from the Northwest of Algeria. Biodiversitas 22: 154-164. Honey bees play an important role as pollinators of many crops. Thus they are collectively considered as a veritable economic source. The present study was undertaken to describe variation in the right forewing geometry in two Algerian honeybee subspecies Apis mellifera intermissa and Apis mellifera sahariensis using landmark-based geometric morphometrics. A total of 1286 honeybees were sampled from 12 provinces in the northwest of Algeria. The forewing geometry was evaluated using 20 homologous landmarks by applying Procrustes superimposition analysis. The top four principal components accounted for only 41.1% of wing shape variation between the two subspecies. There was a significant difference in wing shape between the two subspecies (Mahalanobis distance = 1.0626 ; P<0.001), whereas their wing size seemed similar (P>0.05). Regarding the allometric effect, the percentage of variation in wing shape explained by size changes was relatively small, with 1.28% and 4.37% for A. m. intermissa and A.m sahariensis, respectively. The cross-validation procedure correctly classified 68.3% of specimens into their original groups. PERMANOVA test revealed significant differences in the right forewing shape among all geographic areas studied (P<0.001). The results clearly showed that the landmark-based geometric approach applied to forewings venation is a powerful and reliable tool in the discrimination of native honey bee subspecies and should be considered in local honey bee biodiversity improvement and conservation initiatives.

scholarly journals Characterization of an unusually conserved AluI highly reiterated DNA sequence family from the honeybee, Apis mellifera.

Genetics ◽  
1993 ◽  
Vol 134 (4) ◽  
pp. 1195-1204
Author(s):  
S Tarès ◽  
J M Cornuet ◽  
P Abad
Keyword(s):  
Apis Mellifera ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Repeated Sequence ◽  
Consensus Sequences ◽  
Dna Sequence Data ◽  
Repeat Class ◽  
Honeybee Subspecies

Abstract An AluI family of highly reiterated nontranscribed sequences has been found in the genome of the honeybee Apis mellifera. This repeated sequence is shown to be present at approximately 23,000 copies per haploid genome constituting about 2% of the total genomic DNA. The nucleotide sequence of 10 monomers was determined. The consensus sequences is 176 nucleotides long and has an A + T content of 58%. There are clusters of both direct and inverted repeats. Internal subrepeating units ranging from 11 to 17 nucleotides are observed, suggesting that it could have evolved from a shorter sequence. DNA sequence data reveal that this repeat class is unusually homogeneous compared to the other class of invertebrate highly reiterated DNA sequences. The average pairwise sequence divergence between the repeats is 2.5%. In spite of this unusual homogeneity, divergence has been found in the repeated sequence hybridization ladder between four different honeybee subspecies. Therefore, the AluI highly reiterated sequences provide a new probe for fingerprinting in A. m. mellifera.

scholarly journals Low fertility, fecundity and numbers of mated female offspring explain the lower reproductive success of the parasitic mite Varroa destructor in African honeybees

Parasitology ◽  
2018 ◽  
Vol 145 (12) ◽  
pp. 1633-1639 ◽  
Author(s):  
Beatrice T. Nganso ◽  
Ayuka T. Fombong ◽  
Abdullahi A. Yusuf ◽  
Christian W. W. Pirk ◽  
Charles Stuhl ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Reproductive Success ◽  
Varroa Destructor ◽  
Female Offspring ◽  
Low Fertility ◽  
Mite Infestation ◽  
Unmated Female ◽  
Mite Reproduction ◽  
Worker Brood ◽  
Honeybee Subspecies

AbstractAlthough Varroa destructor is the most serious ecto-parasite to the honeybee, Apis mellifera L., some honeybee populations such as Apis mellifera scutellata in Kenya can survive mite infestations without treatment. Previously, we reported that grooming behaviour could be a potential tolerant mechanism expressed by this honeybee subspecies towards mite infestation. However, both hygienic and grooming behaviours could not explain the lower mite-infestation levels recorded in these colonies. Here, we investigated the involvement of other potential resistant mechanisms including suppression of mite reproduction in worker brood cells of A. m. scutellata to explain the low mite numbers in their colonies. High infertility rates (26–27%) and percentages of unmated female offspring (39–58%) as well as low fecundity (1.7–2.2, average offspring produced) were identified as key parameters that seem to interact with one another during different seasons to suppress mite reproduction in A. m. scutellata colonies. We also identified offspring mortality in both sexes and absence of male offspring as key factors accounting for the low numbers of mated daughter mites produced in A. m. scutellata colonies. These results suggest that reduced mite reproductive success could explain the slow mite population growth in A. m. scutellata colonies.

scholarly journals New Viruses from the Ectoparasite Mite Varroa destructor Infesting Apis mellifera and Apis cerana

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 94 ◽  
Author(s):  
Sofia Levin ◽  
Noa Sela ◽  
Tal Erez ◽  
David Nestel ◽  
Jeffery Pettis ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Varroa Destructor ◽  
Rna Virus ◽  
Apis Cerana ◽  
Virus Genome ◽  
Deformed Wing Virus ◽  
Viral Loads ◽  
Short Period ◽  
Honeybee Subspecies ◽  
Colony Survival

Varroa destructor is an ectoparasitic mite of Asian or Eastern honeybees Apis cerana (A. cerana) which has become a serious threat to European subspecies of Western honeybees Apis mellifera (A. mellifera) within the last century. V. destructor and its vectored honeybee viruses became serious threats for colony survival. This is a short period for pathogen- and host-populations to adapt. To look for possible variation in the composition of viral populations we performed RNA metagenomic analysis of the Western honeybee subspecies A. m. ligustica, A. m. syriaca, A. m. intermissa, and A. cerana and their respective V. destructor mites. The analysis revealed two novel viruses: Varroa orthomyxovirus-1 (VOV-1) in A. mellifera and V. destructor and a Hubei like-virga virus-14 homolog in V. destructor. VOV-1 was more prevalent in V. destructor than in A. mellifera and we found evidence for viral replication in both hosts. Interestingly, we found differences in viral loads of A. cerana and their V. destructor, A. m. intermissa, and its V. destructor showed partial similarity, while A. m. ligustica and A. m. syriaca and their varroa where very similar. Deformed wing virus exhibited 82.20%, 99.20%, 97.90%, and 0.76% of total viral reads in A. m. ligustica, A. m. syriaca, A. m. intermissa, and A. cerana, respectively. This is the first report of a complete segmented-single-stranded negative-sense RNA virus genome in honeybees and V. destructor mites.

scholarly journals Both geometric morphometric and microsatellite data consistently support the differentiation of the Apis mellifera M evolutionary branch

Apidologie ◽  
2011 ◽  
Vol 42 (2) ◽  
pp. 150-161 ◽  
Author(s):  
Irati Miguel ◽  
Michel Baylac ◽  
Mikel Iriondo ◽  
Carmen Manzano ◽  
Lionel Garnery ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Microsatellite Data ◽  
Geometric Morphometric ◽  
Evolutionary Branch
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