scholarly journals High-Throughput Digitisation of Natural History Specimens

Author(s):  
Elizabeth Louise Allan ◽  
Steen Dupont ◽  
Helen Hardy ◽  
Laurence Livermore ◽  
Benjamin Price ◽  
...  

The Natural History Museum, London (NHM) has embarked on an ambitious Digital Collections Programme to digitise its collections. One aim of the programme has been to improve the workflows and infrastructure needed to support high-throughput digitisation and create comprehensive digital inventories of large scientific collections. Pilot projects have been carried out for a variety of collection types, from which high-throughput imaging workflows have been developed and refined. These workflows have focused on pinned insect specimens (Blagoderov et al. 2012, Paterson et al. 2016, Blagoderov et al. 2017, Price et al. 2018), microscope slides (whole slide and specimen imaging; Allan et al. 2018, Allan et al. 2019) and herbarium sheets. The rate and time taken to digitise specimens is influenced by a number of factors that include, among others, the level of preparation and post-processing required, imaging approach, the type of specimens as well as the complexity and condition of the collection. As part of this presentation we will include information on the rate, cost and time to digitise various NHM collections, illustrating how our processes have improved digitisation efficiency and allowed us to maintain quality. The programme has run a variety of digitisation projects, gathering data about rates of digitisation (preparation, imaging, transcription etc.) and developing improvements. Collection types such as microscope slides and herbarium sheets lend themselves to higher imaging rates, while other collections such as pinned insects, which require greater amounts of specimen handling to remove labels, tend to have lower imaging rates (Fig. 1). In order to increase efficiency, we have developed approaches that minimise specimen handling. For example, workflows for pinned insects such as the Angled Label Image Capture and Extraction (ALICE) do not require the removal of specimen labels from the pin as the system can capture angled images of the labels, thus increasing the imaging rate three-fold (Fig. 1). Another approach taken is to semi-automate mass digitisation using a combination of temporary and permanent Data Matrix barcode labels (Allan et al. 2019). By using multiple barcodes at the imaging stage to encode information associated with each specimen (i.e. unique identifier, location in the collection, taxonomic name, type status etc.; Fig. 2), we can run a series of automated processes, including file renaming, image processing and bulk import into the Museum’s collection management system. Through adaptation of our workflows with this new approach we have increased the efficiency of digitisation processes, illustrating how simple activities, like automated file renaming, reduces image post-processing time, minimises human error and can be applied across multiple collection types.

2019 ◽  
Vol 7 ◽  
Author(s):  
E Louise Allan ◽  
Laurence Livermore ◽  
Benjamin Price ◽  
Olha Shchedrina ◽  
Vincent Smith

The Natural History Museum, London (NHM) has embarked on an ambitious programme to digitise its collections. One aim of the programme has been to improve the workflows and infrastructure needed to support high-throughput digitisation and create comprehensive digital inventories of large scientific collections. This paper presents the workflow developed to digitise the entire Phthiraptera (parasitic lice) microscope slide collection (70,663 slides). Here we describe a novel process of semi-automated mass digitisation using both temporary and permanent barcode labels applied before and during slide imaging. By using a series of barcodes encoding information associated with each slide (i.e. unique identifier, location in the collection and taxonomic name), we can run a series of automated processes, including file renaming, image processing and bulk import into the NHM’s collection management system. We provide data on the comparative efficiency of these processes, illustrating how simple activities, like automated file renaming, reduces image post-processing time, minimises human error and can be applied across multiple collection types.


ZooKeys ◽  
2018 ◽  
Vol 795 ◽  
pp. 49-65 ◽  
Author(s):  
Patina K. Mendez ◽  
Sangyeon Lee ◽  
Chris E. Venter

Availability of 3D-printed laboratory equipment holds promise to improve arthropod digitization efforts. A 3D-printed specimen scanning box was designed to image fluid-based arthropod collections using a consumer-grade flatbed scanner. The design was customized to accommodate double-width microscope slides and printed in both Polylactic Acid (PLA) and nylon (Polyamide). The workflow with two or three technicians imaged Trichoptera lots in batches of six scanning boxes. Individual images were cropped from batch imagess using an R script. PLA and nylon both performed similarly with no noticeable breakdown of the plastic; however, dyed nylon leeched color into the ethanol. The total time for handling, imaging, and cropping was ~8 minutes per vial, including returning material to vials and replacing ethanol. Image quality at 2400 dpi was the best and revealed several diagnostic structures valuable for partial identifications with higher utility if structures of the genitalia were captured; however, lower resolution scans may be adequate for natural history collection imaging. Image quality from this technique is similar to other natural history museum imaging techniques; yet, the scanning approach may have wider applications to morphometrics because of lack of distortion. The approach can also be applied to image vouchering for biomonitoring and other ecological studies.


Author(s):  
Steen Dupont ◽  
Benjamin Price

The world’s natural history collections contain at least 2 billion specimens (Ariño 2010), representing a unique data source for answering fundamental scientific questions about ecological, evolutionary, and geological processes. Unlocking this treasure trove of data, stored in thousands of museum drawers and cabinets, is crucial to help map a sustainable future for ourselves and the natural systems on which we depend. The rate-limiting steps in the digitisation of natural history collections often involve specimen handling, due to their fragile nature. Insects comprise the single largest collection type in the Natural History Museum, London (NHM) and in many other collections, reflecting their global diversity and multiplicity. The NHM pinned insect collection, estimated at 25 million specimens, will take over 700 person years to digitise at current rates (Price et al. 2018: estimated from Blagoderov et al. 2017). In order to ramp up digitisation, we have developed ALICE for Angled Label Image Capture and Extraction from pinned insects. This multi-camera setup (Fig. 1) and associated software processing pipeline, enables primary data capture from angled images, without removal of the labels from the specimen pin. As a result ALICE enables a single user to sustainably digitise (add a catalogue label, image and prepare images for database import) over 800 specimens per day (Price et al. 2018), allowing us to digitally unlock large parts of the insect collection (e.g., Hymenoptera, Diptera, Coleoptera) at up to seven times the previous rate. We are continuing to refine hardware approaches to reduce specimen handling and extract data, for both human and machine interpretation, from labels without removing them from the object. More recently we are also trialing multiple mirrors in our Mirror Angled Label Image Capture Equipment (MALICE) (Fig. 2) or a rotating stage for our Vial Image Label Extraction (VILE) (Fig. 3) aimed at spirit-preserved specimens housed in vials. In this talk, we will outline the current approaches in use at the Natural History Museum, next generation prototypes, and challenges that need to be addressed before these techniques can be fully optimized.


2018 ◽  
Author(s):  
Benjamin Wills Price ◽  
Steen Dupont ◽  
Elizabeth Louise Allan ◽  
Vladimir Blagoderov ◽  
Alice Jenny Butcher ◽  
...  

The world’s natural history collections contain at least 2 billion specimens, representing a unique data source for answering fundamental scientific questions about ecological, evolutionary, and geological processes. Unlocking this treasure trove of data, stored in thousands of museum drawers and cabinets, is crucial to help map a sustainable future for ourselves and the natural systems on which we depend. The rate-limiting steps in the digitisation of natural history collections often involve specimen handling due to their fragile nature. Insects comprise the single largest collection type in the Natural History Museum, London (NHM), reflecting their global diversity. The NHM pinned insect collection, estimated at 25 million specimens, will take over 700 person years to digitise at current rates. In order to ramp up digitisation we have developed ALICE for Angled Label Image Capture and Extraction. This multi-camera setup and associated software processing pipeline enables primary data capture from angled images, without removal of the labels from the specimen pin. As a result ALICE enables a single user to sustainably image over 1,000 specimens per day, allowing us to digitally unlock the insect collections at an unprecedented rate.


Zootaxa ◽  
2017 ◽  
Vol 4318 (2) ◽  
pp. 201
Author(s):  
D.J. WILLIAMS

In 1940, E.E. Green’s collection of scale insects, consisting of 6505 microscope slides and 2172 boxes of dry material, was donated to the The Natural History Museum, London, U.K. (then the British Museum (Natural History)). Green was a tea and coffee planter in Sri Lanka, and later became Government Agricultural Entomologist there before retiring to England in 1913. He continued to work on scale insects and became one of the foremost scale insect specialists at the time. His collection includes most of the species he described as new, but is also important because it contains authentic material sent to Green by other contemporary workers on scale insects.                         The collection is listed as it was when originally donated, firstly giving the names of species that Green recognised at the time, followed by the number of microscope slides, followed by numbers of developmental stages in the material; lastly is provided the current name of each species. The list is divided into the 31 extant families represented in Green’s collection. 


Zootaxa ◽  
2017 ◽  
Vol 4363 (1) ◽  
pp. 79 ◽  
Author(s):  
ŁUKASZ KACZMAREK ◽  
ŁUKASZ MICHALCZYK

We examined microscope slides from Horning and Iharos tardigrade collections from Museum of New Zealand Te Papa Tongarewa in Wellington and Hungarian Natural History Museum in Budapest with species of the Macrobiotus hufelandi group. Based on this material we describe one new species, Macrobiotus horningi sp. nov., and re-describe two others, M. maculatus comb. nov. Iharos, 1973 and M. rawsoni Horning et al., 1978. With the oral cavity armature of the patagonicus type and chorion of the hufelandi type, Macrobiotus horningi sp. nov. is most similar to: M. personatus Biserov, 1990, M. sandrae Bertolani & Rebecchi, 1993, M. serratus Bertolani et al., 1996, M. sottilei Pilato et al., 2012, M. terminalis Bertolani & Rebecchi, 1993 and M. vladimiri Bertolani et al., 2011, but it differs from them in morphological and morphometric traits. With eggs of the maculatus type, M. maculatus comb. nov. is most similar to: M. biserovi Bertolani et al., 1996, M. denticulatus Dastych, 2002, M. macrocalix Bertolani & Rebecchi, 1993 and M. ramoli Dastych, 2005, but differs from them in morphological and morphometric characters. 


2021 ◽  
Vol 7 ◽  
Author(s):  
Danail Popov ◽  
Priyanka Roychoudhury ◽  
Helen Hardy ◽  
Laurence Livermore ◽  
Ken Norris

The Natural History Museum, London has been creating digital data about collections for many years, with a formal Digital Collections Programme since 2014. Efforts to monitor the outcomes and impact of this work have focused on metrics of digital access, such as download events, and on citations of digital specimens as a measure of use. Digitisation projects and resulting research have also been used as impact case studies, highlighting areas such as human health and conservation. In 2021, the Museum decided to explore the economic impacts of collections data in more depth, and commissioned Frontier Economics to undertake modelling, resulting in this report. While the methods in this report are relevant to collections globally, this modelling focuses on benefits to the UK, and is intended to support the Museum’s own digitisation work, as well as a current scoping study funded by the Arts & Humanities Research Council about the case for digitising all UK natural science collections as a research infrastructure. This study focuses on digitisation in the round, not distinguishing between different collection types or levels of data creation at this stage. Three methods have been used: first, analysing five key thematic areas or sectors where data from natural science collections are likely to lead to benefits; secondly, analysing typical returns on investment in scientific research; and thirdly, examining the efficiency savings that can be reinvested in research if data are available freely and openly. Together, these methods confirm benefits in excess of £2 billion over 30 years, representing a seven to ten times return on investment.


2004 ◽  
Vol 31 (1) ◽  
pp. 6-29 ◽  
Author(s):  
Richard L. Hodgkinson ◽  
John E. Whittaker

ABSTRACT: In spite of his many other interests, Edward Heron-Allen also worked for nearly 50 years as a scientist on minute shelled protists, called foraminifera, much of it in an unpaid, unofficial capacity at The Natural History Museum, London, and notably in collaboration with Arthur Earland. During this career he published more than 70 papers and obtained several fellowships, culminating in 1919 in his election to the Royal Society. Subsequently, he bequeathed his foraminiferal collections and fine library to the Museum, and both are housed today in a room named in his honour. In this paper, for the first time, an assessment of his scientific accomplishments is given, together with a full annotated bibliography of his publications held in the Heron-Allen Library. This is part of a project to produce a bibliography of his complete publications, recently initiated by the Heron-Allen Society.


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