scholarly journals Pythium huanghuaiense sp. nov. isolated from soybean: morphology, molecular phylogeny and pathogenicity

2021 ◽  
Vol 9 ◽  
Author(s):  
Jia-Jia Chen ◽  
Hui Feng ◽  
Jian Yu ◽  
Wenwu Ye ◽  
Xiaobo Zheng
Keyword(s):  
Glycine Max ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Protein A ◽  
Morphological Characters ◽  
Edible Oil ◽  
Molecular Evidence ◽  
Pathogenicity Test ◽  
Soybean Seedlings ◽  
Thin Walled

Soybean (Glycine max) is a major source of edible oil and protein. A novel species of the genus Pythium, Pythium huanghuaiense, isolated from soybean seedlings in China, is described and illustrated on the basis of morphological characters and molecular evidence. Pythium huanghuaiense sp. nov. is closely related to species of the genus Pythium in clade F, as evidenced by the presence of hyphal swellings and its relatively rapid morphological growth. However, it differs by having relatively small sporangia and plerotic or nearly plerotic and thin-walled oospores. A pathogenicity test confirmed the newly-identified species as a pathogen of soybean.

Three novel species of Russula from southern China based on morphological and molecular evidence

Phytotaxa ◽  
2019 ◽  
Vol 392 (4) ◽  
pp. 264 ◽  
Author(s):  
JING-WEI LI ◽  
JIAN-FEI ZHENG ◽  
YU SONG ◽  
FA YUAN ◽  
LI-HONG QIU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Southern China ◽  
Morphological Characters ◽  
Its Sequences ◽  
Molecular Evidence ◽  
Three New Species

Three new species of Russula collected from southern China are proposed based on morphological characters and phylogenetic analysis of internal transcribed spacer (ITS) sequences. Russula bubalina sp. nov. is characterized by cinnamon buff to pink pileus with striate margin, interveined and forked lamellae, basidiospores with warty ornamentations not forming reticulum, hymenial cystidia becoming brown in sulphovanillin (SV) and slender terminal cells in suprapellis. R. pseudobubalina sp. nov. is closely related to Russula bubalina in macro-morphology, but it can be recognized by its unforked lamellae, shorter cheilocystidia, bigger basidia and basidiospores with lower ornamentations. Russula subatropurpurea sp. nov. can be recognized by the purplish brown pileus, mild-tasted and white context changing to light purplish red with FeSO4, white and forking lamellae without lamellulae, long hymenial cystidia becoming brown in SV. Both morphological and phylogenetic analysis consistently confirmed the distinct positions of three new species in subg. Heterophyllidia subsection Heterophyllinae.

Heteroradulum yunnanensis sp. nov. (Auriculariales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses in China

Phytotaxa ◽  
2020 ◽  
Vol 437 (2) ◽  
pp. 51-59
Author(s):  
QIAN-XIN GUAN ◽  
CHAO-MAO LIU ◽  
TANG-JIE ZHAO ◽  
CHANG-LIN ZHAO
Keyword(s):  
Growth Habit ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Morphological Characters ◽  
Molecular Data ◽  
Fungal Species ◽  
Molecular Evidence ◽  
Monophyletic Lineage ◽  
Thin Walled ◽  
Inference Methods

A new wood-inhabiting fungal species, Heteroradulum yunnanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiomata with odontoid hymenial surface (50–100 µm long), more or less pronounced yellow stains in older basidiomata, a monomitic hyphal system with thin-walled, clamped generative hyphae and two to three-celled basidia and cylindrical, hyaline, thin-walled, smooth, IKI–, CB– basidiospores measuring as 17–24 ×5–8 µm. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that Heteroradulum yunnanensis formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped with H. adnatum.

Phlebia ailaoshanensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses

Phytotaxa ◽  
2018 ◽  
Vol 373 (3) ◽  
pp. 184 ◽  
Author(s):  
SHAN SHEN ◽  
XIANG MA ◽  
TAI-MIN XU ◽  
CHANG-LIN ZHAO
Keyword(s):  
Growth Habit ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Morphological Characters ◽  
Molecular Data ◽  
Molecular Evidence ◽  
Monophyletic Lineage ◽  
Phlebioid Clade ◽  
Thin Walled ◽  
Inference Methods

A new wood-inhabiting fungus, Phlebia ailaoshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with tuberculate to phlebioid hymenial surface, a monomitic hyphal system with slightly thick-walled generative hyphae bearing simple septa, IKI–, CB– and ellipsoid, hyaline, thin-walled, smooth, IKI–, CB– basidiospores measuring as 5.7–8.5 × 3–4.3 µm. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that P. ailaoshanensis belonged to the Meruliaceae and nested into the phlebioid clade. Further investigation was obtained for more representative taxa in the Phlebia based on ITS+nLSU sequences, in which the result demonstrated that the species P. ailaoshanensis formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped with P. acanthocystis, P. chrysocreas, P. ludoviciana, P. subcretacea and P. uda.

A molecular phylogeny of rainbow skinks (Scincidae: Carlia): taxonomic and biogeographic implications

2002 ◽  
Vol 50 (1) ◽  
pp. 39 ◽  
Author(s):  
Devi M. Stuart-Fox ◽  
Andrew F. Hugall ◽  
Craig Moritz
Keyword(s):  
Molecular Phylogeny ◽  
Phylogenetic Signal ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
Previous Suggestion ◽  
Protein Coding ◽  
Single Clade ◽  
Form Part ◽  
Rapid Diversification ◽  
Interspecific Distance

The phylogenetic relationships amongst 29 species of Carlia and Lygisaurus were estimated using a 726-base-pair segment of the protein-coding mitochondrial ND4 gene. Results do not support the recent resurrection of the genus Lygisaurus. Although most Lygisaurus species formed a single clade, this clade is nested within Carlia and includes Carlia parrhasius. Due to this new molecular evidence, and the paucity of diagnostic morphological characters separating the genera, Lygisaurus de Vis 1884 is re-synonymised with Carlia Gray 1845. Our analysis is also inconsistent with a previous suggestion that Lygisaurus timlowi should be removed to Menetia, a genus that is distantly related relative to outgroups used here. Intraspecific variation in Carlia is, in several instances, greater than interspecific distance. The most strikingly divergent lineages are found within C. rubrigularis, which appears to be paraphyletic, with southern populations more closely related to C. rhomboidalis than to northern populations of C. rubrigularis. The two C. rubrigularis–C. rhomboidalis lineages form part of a major polytomy at an intermediate level of divergence. Lack of resolution at this level, however, does not appear to be due to saturation or loss of phylogenetic signal. Rather, the polytomy probably reflects a period of relatively rapid diversification that occurred sometime during the Miocene.

scholarly journals Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446

MycoKeys ◽  
2020 ◽  
Vol 63 ◽  
pp. 163-172
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

scholarly journals Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l.

MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 1-26
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India

Phytotaxa ◽  
2021 ◽  
Vol 525 (3) ◽  
pp. 205-222
Author(s):  
SANJEET KUMAR VERMA ◽  
SANJAY YADAV ◽  
RAGHVENDRA SINGH
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Murraya Koenigii ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Asexual State

Aplosporella abexaminans, a new sexual morph fungus belongs to family Aplosporellaceae, was discovered on the bark of stem of Murraya koenigii (Rutaceae) and identified by morphological characteristics and analysis of combined ITS and LSU sequence data. This is the second report of a sexual morph with molecular evidence for this genus and the second record of conidiogenesis and chlamydospores associated with the asexual state of this family. It is characterized by its larger ascostromata, locules without ostioles, thinner locule peridium, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, larger and hyaline to light olivaceous ascospores, asexual state without conidiomata formation, conidiogenous cells light brown to brown, conidia brown without granular content, smooth and presence of chlamydospores. The sexual morph of Aplosporella abexaminans resembles Bagnisiella and the asexual morph resembles Aplosporella, thus proving the sexual-asexual connection for the second time for this family.

scholarly journals Two new species of Fuscoporia (Hymenochaetales, Basidiomycota) from southern China based on morphological characters and molecular evidence

MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 75-89
Author(s):  
Qian Chen ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Related Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
Two New Species ◽  
Thin Walled ◽  
The Individual

Fuscoporia (Hymenochaetaceae) is characterized by annual to perennial, resupinate to pileate basidiocarps, a dimitic hyphal system, presence of hymenial setae, and hyaline, thin-walled, smooth basidiospores. Phylogenetic analyses based on the nLSU and a combined ITS, nLSU and RPB2 datasets of 18 species of Fuscoporia revealed two new lineages that are equated to two new species; Fuscoporia ramulicolasp. nov. grouped together with F. ferrea, F. punctatiformis, F. subferrea and F. yunnanensis with a strong support; Fuscoporia acutimarginatasp. nov. formed a strongly supported lineage distinct from other species. The individual morphological characters of the new species and their related species are discussed. A key to Chinese species of Fuscoporia is provided.

Morphological characteristics and phylogenetic analyses reveal Antrodia yunnanensis sp. nov. (Polyporales, Basidiomycota) from China

Phytotaxa ◽  
2020 ◽  
Vol 460 (1) ◽  
pp. 1-11
Author(s):  
MEI-LING HAN ◽  
QI AN ◽  
WEN-XIAN FU ◽  
XUAN CHENG ◽  
TIAN BU ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Sister Group ◽  
Southwestern China ◽  
Molecular Evidence ◽  
Pore Surface ◽  
Hyphal System ◽  
Thin Walled

A new polypore, Antrodia yunnanensis, collected from southwestern China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by annual, resupinate basidiocarps with greyish blue to dark greyish blue pore surface upon drying, round to angular pores measuring 2–3 per mm, a dimitic hyphal system with clamped generative hyphae, subicular hyphae bearing fine crystals, and cylindrical, thin-walled, smooth basidiospores measuring 7–9.9 × 2.5–3.1 µm. Molecular phylogeny inferred from ITS and nLSU sequence data showed that samples of A. yunnanensis formed a distinctive lineage in Antrodia sensu lato, and it nested a sister group with A. tropica.

Porpomyces submucidus (Hydnodontaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidence

Phytotaxa ◽  
2015 ◽  
Vol 230 (1) ◽  
pp. 61 ◽  
Author(s):  
Fang Wu ◽  
CHANG-LIN ZHAO
Keyword(s):  
New Species ◽  
Sister Group ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
Its Sequence ◽  
Thin Walled ◽  
Tropical China ◽  
A New Species

A new species is described from tropical China as Porpomyces submucidus on the basis of both morphological characters and molecular evidence. Phylogenetic analyse based on the ITS sequence, LSU sequence and the ITS+LSU sequence show that the new species belongs to Porpomyces and forms a distict clade as a sister group of Porpomyces mucidus. The fungus is characterized by thin, white to cream, resupinate basidiome with cottony to rhizomorphic margin, small pores (7–9 per mm), a monomitic hyphal structure with clamped generative hyphae, usually ampullated at most septa, and small, hyaline, thin-walled, ellipsoid basidiospores measuring 2.2–2.8 × 1.2–1.8 μm. It is closely related to Porpomyces mucidus, but the latter has larger pores (4–5 per mm) and larger basidiospores (2.8–3.9 × 2–2.8 μm).

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