scholarly journals A revision of European species of the genus Tetrastichus Haliday (Hymenoptera: Eulophidae) using integrative taxonomy

2020 ◽  
Vol 8 ◽  
Author(s):  
Christer Hansson ◽  
Stefan Schmidt
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Host Record ◽  
Current Data ◽  
Biological Information ◽  
Morphological Data ◽  
Integrative Approach ◽  
Similar Species ◽  
European Species ◽  
Wide Range

The European species of the genus Tetrastichus (Insecta, Hymenoptera, Eulophidae, Tetrastichinae) are revised with 93 species, including 50 species described as new. The revision was conducted using an integrative taxonomic approach, based on DNA barcoding in combination with morphological characters. The Tetrastichinae are a biologically diverse and species-rich group of parasitoid wasps with numerous complexes of morphologically often very similar species that attack a wide range of hosts in over 100 insect families in 10 different orders. The genus Tetrastichus is, with almost 500 described species, the third largest genus of Tetrastichinae. Although biological information is lacking for most species, current data indicate that Tetrastichus species are gregarious koinobiont endoparasitoids developing on juvenile stages of mainly holometabolous insects. Due to their host specificity, several species of Tetrastichus are used as biological control agents. The European species of Tetrastichus Haliday (Hymenoptera: Eulophidae) are revised using a combination of externo-morphological and DNA barcoding data. This is the first integrative approach for any of the large genera of the Tetrastichinae. A total of 93 species are included, of which 50 are described as new: T. agonus sp. n., T. antonjanssoni sp. n., T. argei sp. n., T. argutus sp. n., T. asilis sp. n., T. ballotus sp. n., T. bledius sp. n., T. broncus sp. n., T. calcarius sp. n., T. calmius sp. n., T. clisius sp. n., T. cosidis sp. n., T. cumulus sp. n., T. cyprus sp. n., T. delvarei sp. n., T. doczkali sp. n., T. elanus sp. n., T. elodius sp. n., T. ennis sp. n., T. enodis sp. n., T. erinus sp. n., T. evexus sp. n., T. fadus sp. n., T. fenrisi sp. n., T. flaccius sp. n., T. gredius sp. n., T. iasi sp. n., T. illydris sp. n., T. incanus sp. n., T. inscitus sp. n., T. intruitus sp. n., T. johnnoyesi sp. n., T. lacustrinus sp. n., T. ladrus sp. n., T. lanius sp. n., T. lazius sp. n., T. lixalius sp. n., T. lycus sp. n., T. marcusgrahami sp. n., T. minius sp. n., T. mixtus sp. n., T. nataliedaleskeyae sp. n., T. nymphae sp. n., T. pixius sp. n., T. scardiae sp. n., T. splendens sp. n., T. sti sp. n., T. suecus sp. n., T. tacitus sp. n. and T. tartus sp. n. Two keys for the identification of species are presented, one for females and one for males. Based on DNA barcode sequences for 70 of the species, a Maximum Likelihood tree to assess phylogenetic relationships within the genus is presented. These 70 species are also characterised by a combination of CO1 and morphological data. The remaining 23 species, without a DNA barcode, are characterised by morphological data. Using a combination of data from the morphology and CO1 or morphological data only, the species are separated into three species groups (clito-, hylotomarum-, murcia-groups) with 41 unplaced species outside these groups. Hosts are known for 27 of the species and they are gregarious, koinobiont endoparasitoids on a wide range of immature stages of holometabolous insects and appear to be very host specific. The first host record for Lepidoptera (Tineidae) in Europe is included.

A comprehensive and integrative re-description of Synchaeta tremula (Müller, 1786) and the newly rediscovered Synchaeta tremuloida Pourriot, 1965 (Rotifera: Synchaetidae)

Zootaxa ◽  
2017 ◽  
Vol 4276 (4) ◽  
pp. 503 ◽  
Author(s):  
TANJA WILKE ◽  
WILKO H. AHLRICHS ◽  
OLAF R.P. BININDA-EMONDS
Keyword(s):  
Taxonomic Status ◽  
Morphological Data ◽  
Integrative Approach ◽  
Similar Species ◽  
Ecological Data ◽  
Separate Species ◽  
Unique Character ◽  
Character Set ◽  
Initial Description ◽  
Scanning Electron

In this paper, we focus on the two morphologically similar species Synchaeta tremula (Müller, 1786) and Synchaeta tremuloida Pourriot, 1965. This study records one of the first detections of the latter species since its initial description and clarifies its uncertain taxonomic status. Using an integrative approach combining morphological data (from light and scanning electron microscopy) with molecular and ecological data, we present re-descriptions of S. tremula and S. tremuloida and confirm their status as separate species. Finally, we also provide a unique character set that can be readily applied to unambiguously identify each species using easily observable features. 

scholarly journals Integrative taxonomy reveals disjunct distribution and first record of Hoplias misionera (Characiformes: Erythrinidae) in the Amazon River basin: morphological, DNA barcoding and cytogenetic considerations

2021 ◽  
Vol 19 (2) ◽  
Author(s):  
Karen L. A. Guimarães ◽  
Juan J. Rosso ◽  
Mendelsohn F. B. Souza ◽  
Juan M. Díaz de Astarloa ◽  
Luís R. R. Rodrigues
Keyword(s):  
Dna Barcoding ◽  
Amazon Basin ◽  
First Record ◽  
Morphological Data ◽  
Disjunct Distribution ◽  
Valid Species ◽  
Integrative Approach ◽  
Molecular Evidence ◽  
La Plata ◽  
La Plata Basin

Abstract The Hoplias malabaricus group encompasses six valid species and still is believed to harbors cryptic diversity. In this work, an integrative approach including morphological, DNA barcoding, and cytogenetic considerations was conducted to characterize a population of H. malabaricus from the Amazon basin that was recently allocated in the same mitochondrial lineage with H. misionera, a species originally described from La Plata basin. The DNA barcoding analysis revealed that the Amazon population nested together with H. misionera specimens from the La Plata basin (BIN AAB1732) in the same cluster. The intragroup distance (0.5%) was 12 times lower than the nearest neighbor (6%) distance. The morphometric analysis demonstrated slightly variation between Amazon and La Plata populations, being the former composed by larger specimens. Further morphological data supported the molecular evidence of H. misionera inhabiting Amazon basin. The karyotype characterization of H. misionera in the Amazon population showed 2n=40 and karyotypic formulae 20m+20sm, that added to C-banding, Ag-NOR and 18S results are suggestive of the similarity to karyomorph C of H. malabaricus. This work reveals the first record of H. misionera outside of La Plata basin and expands the species distribution for 2500 km northward until the Marajó Island, estuary of Amazonas River.

scholarly journals Contribution of DNA barcoding to the study of the bees (Hymenoptera: Apoidea) of Canada: progress to date

2017 ◽  
Vol 149 (6) ◽  
pp. 736-754 ◽  
Author(s):  
Cory S. Sheffield ◽  
Jennifer Heron ◽  
Jason Gibbs ◽  
Thomas M. Onuferko ◽  
Ryan Oram ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Analysis ◽  
Dna Barcode ◽  
Ecological Studies ◽  
Wide Range ◽  
Bee Diversity ◽  
Cryptic Taxa ◽  
Morphological Species ◽  
Taxonomic Studies ◽  
Nesting Preferences

AbstractBees (Hymenoptera: Apoidea, Apiformes) are taxonomically and ecologically diverse, with a wide range of social complexity, nesting preferences, floral associations, and biogeographic restrictions. A Canadian bee checklist, greatly assisted by the gene-assisted approach of DNA barcoding, is nearing completion. Previous evaluation of bee diversity in Canada, assisted by DNA barcoding, was restricted to Nova Scotia, which contains about 25% of the bee species in the country. Here, we summarise efforts to date to build a comprehensive DNA barcode library supporting bee taxonomic studies in Canada, consisting of more than 12 500 barcode-compliant sequences yielding 811 distinct barcode index numbers (BINs). This appears to represent ~95% of the 856 bee species presently recorded from Canada, but comparison with known morphological species in each genus shows that some genera are still under-sampled or may contain cryptic taxa, with much taxonomic work still to be done on bees in Canada. This is particularly true within the taxonomically difficult generaAndrenaFabricius (Andrenidae), HylaeusFabricius (Colletidae),MelissodesLatreille (Apidae),NomadaScopoli (Apidae),OsmiaPanzer (Megachilidae), andSphecodesLatreille (Halictidae). DNA analysis will likely be a key asset in resolving bee taxonomic issues in Canada in the future, and to date has even assisted studies of well-known bee taxa. Here we present summaries of our results, and discuss the use of DNA barcoding to assist future taxonomic work, faunal lists, and ecological studies.

scholarly journals Single-Laboratory Validation of a Two-Tiered DNA Barcoding Method for Raw Botanical Identification

2019 ◽  
Vol 102 (5) ◽  
pp. 1435-1447 ◽  
Author(s):  
Zhengfei Lu ◽  
Christopher M Thompson ◽  
Tiffany Chua ◽  
Silva Babajanian ◽  
Yanjun Zhang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Raw Materials ◽  
Dna Barcode ◽  
Sampling Technique ◽  
Regulatory Compliance ◽  
Raw Material ◽  
Animal Products ◽  
Policy Makers ◽  
Plant Parts ◽  
Wide Range

Abstract Background: The applications of deoxyribonucleic acid (DNA) barcoding methods have been extended from authenticating taxonomic provenance of animal products to identifying botanicals used as herbal medicine and in botanical dietary supplements. DNA barcoding methods for botanical identification must be adequately validated to meet regulatory compliance. Objective: The goal of this study is to provide a validation protocol for a two-tiered DNA barcoding method that aims to identify raw botanicals. Methods: A barcode database was computationally validated to define the barcode combinations that can unambiguously identify botanicals in the database. A maximum variation sampling technique was used to capture a wide range of perspectives relating to DNA barcode-based botanical identification, including plant parts and species distance, for the experimental validation. Twenty-two authenticated botanicals were purposively sampled from different plant parts—covering both closely related and distantly related species—to validate the two-tiered DNA barcoding method. The performance of the method was assessed on accuracy, precision, ruggedness, and uncertainty. Results: High accuracy (100%) and precision (1.0) were obtained from the validation samples. The method was also found to be rugged and have acceptable uncertainty. Conclusions: The method was validated and suitable for DNA-based identification of botanical raw materials listed in the current database. Highlights: This work will provide support guidance for manufacturers and regulatory policy makers to implement equivalent validated and compliant DNA-based testing in quality control processes to improve botanical raw material identification and authentication.

scholarly journals Neither molecular nor morphological data have all the answers; with an example from Macrobrachium (Decapoda: Palaemonidae) from Australia

Zootaxa ◽  
2011 ◽  
Vol 2874 (1) ◽  
pp. 65 ◽  
Author(s):  
TIMOTHY J. PAGE ◽  
JANE M. HUGHES
Keyword(s):  
Dna Barcoding ◽  
Molecular Data ◽  
Morphological Data ◽  
Integrative Approach ◽  
Max Planck ◽  
Call And Response

Much controversy still seems to surround the role of molecular data in general, and DNA barcoding in particular, within the taxonomic community. This has lead to numerous “call and response” pairs of papers, most recently Ebach (2011) and Mitchell (2011), but preceded by many other pairs, such as Packer et al. (2009) and Hołyński (2010). There have been numerous calls for a more “integrative” approach to taxonomy (Mitchell 2011; Stevens et al. 2011), which itself has generated point and counterpoint papers (Dayrat 2005; Valdecasas et al. 2008). This of course is how science progresses (although Max Planck suggested that science advances “one funeral at a time”, en.wikiquote.org/wiki/Max_Planck, accessed 20 March 2011).

Synaptus iranicus sp. nov., a second species of the genus Synaptus Eschscholtz, 1829 from Iran (Coleoptera: Elateridae) discovered by an integrative approach

Zootaxa ◽  
2017 ◽  
Vol 4232 (4) ◽  
pp. 568 ◽  
Author(s):  
ANDREA JARZABEK-MÜLLER ◽  
JÉRÔME MORINIÈRE ◽  
HASSAN BARIMANI VARANDI ◽  
JÖRG MÜLLER
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Single Species ◽  
Integrative Approach ◽  
Index Number ◽  
Similar Species ◽  
Distinctive Features ◽  
Coi Barcoding ◽  
A New Species

Synaptus filiformis Fabricius, 1781 has been recognized as a rather variable elaterid species. Based on morphological distinctness of 3 specimens (2 males, 1 female) from Mazandaran, Iran, a new species is here hypothesised. COI barcoding supports the new species as a new Barcode Index Number with a distance of mean 13.5% to the three other BINs available on the Barcode of Life Database. The new species Synaptus iranicus sp. nov. (BOLD: ACZ9929) and its distinctive features are described. Moreover the results of the DNA barcoding suggest that Synaptus filiformis as yet described is not a single species, but rather a complex of several morphologically similar species. 

scholarly journals Hydrodroma angelieri (Acari, Hydrachnidia: Hydrodromidae) a new water mite species from Corsica based on morphological and DNA barcode evidence

Acarologia ◽  
2022 ◽  
Vol 62 (1) ◽  
pp. 3-11
Author(s):  
Vladimir Pešić ◽  
Harry Smit
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Distinct Species ◽  
Mite Species ◽  
Morphological Data ◽  
Dna Barcodes ◽  
Water Mite ◽  
European Species ◽  
A New Species ◽  
High Genetic Distance

In the present study we used morphological data and DNA barcodes to describe a new species, Hydrodroma angelieri sp. nov. from Corsica, France. A high genetic distance of 17.3±0.017% K2P from its molecularly most closely related European congener, H. despiciens (Müller, 1776), supports H. angelieri sp. nov. as a distinct species. Morphologically the new species can be identified on the basis of relatively small leg claws, the presence of only one swimming seta on II-L-5 and 4-6 swimming setae on the anterior surface of IV-L-5. An updated key for the European species of Hydrodroma is provided.

scholarly journals Unheeded biodiversity: perspectives of DNA barcoding of Trichoptera in Bosnia and Herzegovina

2021 ◽  
Vol 4 ◽  
Author(s):  
Dalila Destanović ◽  
Lejla Ušanović ◽  
Jasna Hanjalić ◽  
Lejla Lasić ◽  
Belma Kalamujić Stroil
Keyword(s):  
Species Diversity ◽  
Dna Barcoding ◽  
Bosnia And Herzegovina ◽  
Dna Barcode ◽  
Freshwater Ecosystems ◽  
Morphological Data ◽  
Morphological Description ◽  
General Notion ◽  
Sequential Data ◽  
Public Data

Bosnia and Herzegovina has valuable natural resources with a high percentage of endemic and autochthonous species (Kučinić et al. 2008, Đug and Drešković 2012). The freshwater fauna of Trichoptera in this area is under-investigated, with a lack of morphological description of different life stages and DNA barcode data. Public data show 58,993 barcode entries for Trichoptera in the Barcode of Life Data Systems (BOLD) submitted from 92 countries, and none from Bosnia and Herzegovina (B&H) (BOLD 2021). Previous research in Bosnia and Herzegovina has provided the first DNA barcode for the endemic species Rhyacophila bosnica, stored in GeneBank, under accession number MK211322 by a domestic institution (Kalamujić Stroil et al. 2018). A few DNA barcodes of adult individuals of Trichoptera from Bosnia and Herzegovina were found in BOLD. However, these specimens were collected on B&H territory, but analyzed, processed, and stored by foreign institutions. To change the current state of DNA barcoding of Trichoptera in Bosnia and Herzegovina, we aimed to employ this approach in investigating caddisflies in selected habitats in the Sarajevo Canton. Our fieldwork was done in all five protected areas (spring of the Bosna River, Bijambare, Trebević, Skakavac, and Bentbaša) in which larvae samples were collected according to the AQEM sampling methodology. The standard animal DNA barcode was successfully obtained using degenerated primers LCO1490- JJ and HCO2198-JJ (Astrin and Stüben 2008). Out of 684 collected individuals (313 Trebević, 130 spring of the Bosna River, 117 Bijambare, 71 Bentbaša, 53 Skakavac), a subset of specimens were sequenced. We uncovered 14 different taxa, 11 genera and six families (Limnephilidae, Glossosomatidae, Rhyacophilidae, Goeridae, Hydropsychidae, Polycentropodidae). The preliminary data of Trichoptera composition in the Sarajevo Canton indicated species richness. Based on our sequential data, a new subspecies was discovered in two investigated areas (Valladolid et al. 2020), proving that Trichoptera species diversity in our country is far from entirely uncovered. The benefit and power of the DNA barcoding approach are that it can pinpoint the areas of vast and unknown species diversity more economically, both financially and temporarily, than the morphological approach. Therefore, we believe that it is critical to support the development of DNA barcoding for the bioassessment of freshwater ecosystems in Bosnia and Herzegovina. Several problems prevented us from exploiting sequential data to the fullest. Despite a general notion among scientists that European Trichoptera species are well covered in the BOLD database, most of the sequences we obtained were absent from the database. Secondly, we recognized that morphological data about the larval developmental stage of B&H Trichoptera species are largely missing. The unified, updated, and complete data on this order of insects is urgently needed. However, insufficient financial support by governmental institutions and lack of systematic approach to barcoding the wildlife of Bosnia and Herzegovina hampers this process. Further attempts to collaborate with the stakeholders can be crucial with profound and substantial implications for biomonitoring of aquatic macroinvertebrates in general. New approaches, such as novel DNA barcoding-based methodology can fill an important gap in our knowledge of Balkan caddisflies haplotypes, lineages, and their diversification and distribution patterns.

The Management of Post-Tonsillectomy Morbidity in Patients With Sleep Apnoea

2019 ◽  
Vol 70 (10) ◽  
pp. 3738-3740
Keyword(s):  
Day Surgery ◽  
Sleep Apnoea ◽  
Integrative Approach ◽  
Multicenter Studies ◽  
Therapeutic Modalities ◽  
Advantages And Disadvantages ◽  
Obstructive Sleep ◽  
Wide Range ◽  
The Impact ◽  
Surgery Center

The Tonsillectomy in children or adults is an intervention commonly encountered in the ENT (Ear Nose and Throat) and Head and Neck surgeon practice. The current tendency is to perform this type of surgery in major ambulatory surgery centers. Two objectives are thus pursued: first of all, the increase of the patient quality of life through the reintegration into the family as quickly as possible and secondly, the expenses associated with continuous hospitalization are reduced. Any tertiary (multidisciplinary) sleep center must ensure the complete diagnosis and treatment (including surgery) of sleep respiratory disorders. Under these conditions the selection of patients and especially the implementation of the specific protocols in order to control the postoperative complications it becomes essential. The present paper describes our experience of tonsillectomy as treatment for selected patients with chronic rhonchopathy (snoring) and mild to moderate obstructive sleep apnoea. It was presented the impact of antibiotics protocols in reducing the main morbid outcomes following tonsillectomy, in our day surgery center. The obtained results can also be a prerequisite for the integrative approach of the patients with sleep apnoea who were recommended surgical treatment. Considering the wide range of therapeutic modalities used in sleep apnoea, each with its specific advantages and disadvantages, more extensive and multicenter studies are needed. Keywords: post-tonsillectomy morbidity, day surgery center, sleep disorders

Nanoparticles Functionalized with Venom-Derived Peptides and Toxins for Pharmaceutical Applications

2020 ◽  
Vol 21 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Ana P. dos Santos ◽  
Tamara G. de Araújo ◽  
Gandhi Rádis-Baptista
Keyword(s):  
Oral Absorption ◽  
Current Data ◽  
Pharmacological Activities ◽  
Venom Peptides ◽  
Essential Components ◽  
Wide Range ◽  
Different Types ◽  
Direct Use ◽  
Animal Venoms ◽  
Pharmaceutical Biotechnology

Venom-derived peptides display diverse biological and pharmacological activities, making them useful in drug discovery platforms and for a wide range of applications in medicine and pharmaceutical biotechnology. Due to their target specificities, venom peptides have the potential to be developed into biopharmaceuticals to treat various health conditions such as diabetes mellitus, hypertension, and chronic pain. Despite the high potential for drug development, several limitations preclude the direct use of peptides as therapeutics and hamper the process of converting venom peptides into pharmaceuticals. These limitations include, for instance, chemical instability, poor oral absorption, short halflife, and off-target cytotoxicity. One strategy to overcome these disadvantages relies on the formulation of bioactive peptides with nanocarriers. A range of biocompatible materials are now available that can serve as nanocarriers and can improve the bioavailability of therapeutic and venom-derived peptides for clinical and diagnostic application. Examples of isolated venom peptides and crude animal venoms that have been encapsulated and formulated with different types of nanomaterials with promising results are increasingly reported. Based on the current data, a wealth of information can be collected regarding the utilization of nanocarriers to encapsulate venom peptides and render them bioavailable for pharmaceutical use. Overall, nanomaterials arise as essential components in the preparation of biopharmaceuticals that are based on biological and pharmacological active venom-derived peptides.

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