scholarly journals DNA barcoding reveals an unknown Chironomidae diversity from the freshwater biodiversity hot-spot: comparison between local and the European datasets

2021 ◽  
Vol 4 ◽  
Author(s):  
Piotr Gadawski ◽  
Matteo Montagna ◽  
Bruno Rossaro ◽  
Wojciech Giłka ◽  
Vladimir Pesic ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Hot Spot ◽  
Distribution Patterns ◽  
Index Number ◽  
Lake Basin ◽  
Freshwater Biodiversity ◽  
Biting Midges ◽  
Chironomid Species ◽  
Traditional Taxonomy ◽  
Lake Skadar

In the present study, we developed and evaluated the first reference barcode library for non-biting midges (Diptera: Chironomidae) as flagship taxa of freshwater ecology from Skadar Lake system (Montenegro and Albania), a well-known hotspot of freshwater biodiversity composed by the young lake Skadar (originated 1200 before present) and by its old system of springs (originated during Pliocene). Using an expanded reference library and records deposited in Barcode of Life Database (BOLD), we estimated DNA barcoding efficiency for the European Chironomidae. Study provides COI barcodes for 770 Chironomidae individuals assigned, based on morphology, to 77 species collected in the Skadar Lake basin. Molecular analyses assigned sequences to 100 BINs and 104 OTUs (all records from this area are new for online repositories) and confirms the usefulness of DNA barcoding for the identification of non-biting midges. Additionally, we explored chironomid species distribution patterns in Europe using universal Barcode Index Number (BIN) with a discussion of problematic species groups, both for traditional taxonomy and DNA barcoding. The results of our study provide the first insight into the factual chironomid species diversity of the Lake Skadar basin, in comparison with chironomid fauna at the European scale. The results fill a significant gap in knowledge of biodiversity in the Balkan region. Based on the results of Chironomidae fauna investigation, we conclude that the Skadar Lake basin is now well sampled and such a high representation of species from various sampling sites provides reliable estimation of the non-biting midges fauna.

scholarly journals DNA barcode library revealed unknown diversity of chironomid midges in Montenegro

2021 ◽  
Vol 4 ◽  
Author(s):  
Piotr Gadawski ◽  
Bruno Rossaro ◽  
Wojciech Giłka ◽  
Tomasz Rewicz ◽  
Giulia Magoga ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Dna Barcode ◽  
Lake Basin ◽  
Freshwater Biodiversity ◽  
Biting Midges ◽  
First Results ◽  
Chironomid Midges ◽  
Balkan Region ◽  
Further Development ◽  
Insight Into

We present the first results of the study aiming to investigate the diversity of the non-biting midges (Diptera: Chironomidae) fauna of the Skadar Lake system (Montenegro and Albania), a well-known hotspot of freshwater biodiversity composed by the young lake Skadar (originated 1200 before present) and by its old system of springs (originated during Pliocene). During the study, 8,147 COI barcodes were obtained and revealed the presence of 474 BINs and 586 OTUs assigned to 148 species. Our results provide the first insight into the factual molecular diversity among chironomids inhabiting Skadar Lake basin and fill a significant gap in the knowledge of the biodiversity in the Balkan region. With 219 (46.2%) unique BINs from the Skadar Lake basin new for BOLD, we can expect that further development of barcode reference libraries will help to bind unidentified developmental stages with those identified based on morphology and will reveal hidden Chironomidae species diversity. Further studies should be focused on sampling developmental stages which provides the best species-level resolution, such as mature males. It will help to develop a reliable reference barcode library - fundamental during further assessments.

Exploring the utility of DNA barcoding in species delimitation of Polypedilum (Tripodura) non-biting midges (Diptera: Chironomidae) 

Zootaxa ◽  
2016 ◽  
Vol 4079 (5) ◽  
pp. 534 ◽  
Author(s):  
CHAO SONG ◽  
QIAN WANG ◽  
RUILEI ZHANG ◽  
BINGJIAO SUN ◽  
XINHUA WANG
Keyword(s):  
Dna Barcoding ◽  
Species Delimitation ◽  
Biting Midges

scholarly journals Pendugaan Kejadian Pemutihan Karang Berdasarkan Analisis Suhu Permukaan Laut (SPL) Tahun 2015-2016 di Perairan Bali

2017 ◽  
Vol 4 (2) ◽  
pp. 286
Author(s):  
Jajang Nuryana ◽  
I Gede Hendrawan ◽  
Widiastuti Karim
Keyword(s):  
Coral Bleaching ◽  
Hot Spot ◽  
Satellite Image ◽  
Distribution Patterns ◽  
Triangle Center ◽  
Moderate Resolution ◽  
Resolution Imaging ◽  
Moderate Resolution Imaging Spectroradiometer ◽  
The Difference ◽  
Level 2

National Ocean Atmospheric Administrations (NOAA) by the program coral reef Watch (CRW) has developed a method to estimate the potential of coral bleaching using Sea Surface Temperature (SST). The products are hot spot (HS) and degree heating week (DHW). HS is the SST 1°C (SSTL?1) above normal and DHW is the length of HS inhabits a place. The CRW product do not provided detail informations because it has a lower resolution. It is need a satellite image with a higher resolution to provide better informations. One of the satellite images that can be used is Moderate Resolution Imaging Spectroradiometer (MODIS) with a spatial resolution of 1 km. The purpose of this study was to know HS and DHW distribution patterns and status of coral bleaching in Bali waters seen from the analysis of HS and DHW. MODIS data is used daily, then do mosaicing process to get a weekly SPL (8 daily) and the monthly SST. Monthly SPL normally used to get maximum montly mean (MMM). HS obtained from the difference between 8 daily weekly SST and SST normal (MMM).).Location bleaching based on data Coral Triangle Center (CTC) and coralwatch.org.  SST results revealed difference of SPL in 2015 and 2016 amounted to 1.48°C. Highest DHW in Bali Hai, Nusa Penida is 10 465° C-weeks in April 2016. Based on the value HS and DHW coral reefs in Bali waters threatened bleaching level Alert 1 and Alert level 2.

scholarly journals Integrating DNA Barcoding and Traditional Taxonomy for the Identification of Dipterocarps in Remnant Lowland Forests of Sumatra

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 461 ◽  
Author(s):  
Carina Carneiro de Melo Moura ◽  
Fabian Brambach ◽  
Kevin Jair Hernandez Bado ◽  
Konstantin V. Krutovsky ◽  
Holger Kreft ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Evolutionary Rate ◽  
Molecular Data ◽  
Morphological Identification ◽  
Chloroplast Gene ◽  
Taxonomic Level ◽  
Morphological Taxonomy ◽  
Traditional Taxonomy ◽  
Lowland Forests ◽  
Interspecific Genetic Distance

DNA barcoding has been used as a universal tool for phylogenetic inferences and diversity assessments, especially in poorly studied species and regions. The aim of this study was to contrast morphological taxonomy and DNA barcoding, using the three frequently used markers matK, rbcL, and trnL-F, to assess the efficiency of DNA barcoding in the identification of dipterocarps in Sumatra, Indonesia. The chloroplast gene matK was the most polymorphic among these three markers with an average interspecific genetic distance of 0.020. The results of the molecular data were mostly in agreement with the morphological identification for the clades of Anthoshorea, Hopea, Richetia, Parashorea, and Anisoptera, nonetheless these markers were inefficient to resolve the relationships within the Rubroshorea group. The maximum likelihood and Bayesian inference phylogenies identified Shorea as a paraphyletic genus, Anthoshorea appeared as sister to Hopea, and Richetia was sister to Parashorea. A better discriminatory power among dipterocarp species provided by matK and observed in our study suggests that this marker has a higher evolutionary rate than the other two markers tested. However, a combination of several different barcoding markers is essential for reliable identification of the species at a lower taxonomic level.

scholarly journals Biological aspects of the associations of biting midges (Diptera: Ceratopogonidae) in two saline rivers of the Elton Lake Basin, Russia

2018 ◽  
Vol 69 (6) ◽  
pp. 906 ◽  
Author(s):  
Larisa V. Golovatyuk ◽  
Tatiana D. Zinchenko ◽  
Nadezhda N. Sushchik ◽  
Galina S. Kalachova ◽  
Michail I. Gladyshev
Keyword(s):  
Organic Matter ◽  
Dry Weight ◽  
Lake Basin ◽  
Biting Midges ◽  
Nutritive Quality ◽  
Volgograd Region ◽  
Saline Rivers ◽  
The Family ◽  
Biological Aspects ◽  
First Time

We studied species composition, density, biomass and production of larvae of the family Ceratopogonidae in two saline rivers (Volgograd region, Russia). Ceratopoponids make up an important part of macroinvertebrate community in these rivers. Average monthly production (dry weight) of ceratopogonid larvae in the rivers was 3.5–4.8gm–2 month–1 in May and ~0.9gm–2 month–1 in August. For the first time, feeding spectra of ceratopogonid larvae, Palpomyia schmidti Goetghebuer, 1934, was studied using fatty acid analyses. The larvae of P. schmidti appeared to selectively consume diatoms and other algae and to avoid bacteria and decomposed dead organic matter (detritus) of low nutritive quality.

scholarly journals DNA barcoding and traditional taxonomy unified through Integrative Taxonomy: a view that challenges the debate questioning both methodologies

2010 ◽  
Vol 10 (2) ◽  
pp. 339-346 ◽  
Author(s):  
Amanda Ciprandi Pires ◽  
Luciane Marinoni
Keyword(s):  
Dna Barcoding ◽  
Financial Incentives ◽  
Scientific Community ◽  
New Technologies ◽  
Integrative Taxonomy ◽  
Taxonomic Impediment ◽  
Traditional Taxonomy

The taxonomic crisis, emphasized in recent years, is marked by the lack of popularity (lack of interest in taxonomy) and financial incentives to study biodiversity. This situation, coupled with the issues involved with the necessity of knowing many yet undiscovered species, has meant that new technologies, including the use of DNA, have emerged to revitalize taxonomy. Part of the scientific community, however, has rejected the use of these innovative ideas. DNA barcoding has especially been the target of numerous criticisms regarding its application, as opposed to the use of morphology. This paper aims to highlight the inconsistency of the debate involving DNA versus morphology, since there is a proposal for the integration of traditional taxonomy and DNA barcoding - the integrative taxonomy. The positive and negative points of this proposal will be discussed, as well as its validity and application. From it, the importance of morphology is recognized and the revitalization of traditional taxonomy is achieved by the addition of technologies to overcome the taxonomic impediment.

scholarly journals Diversity in a Cold Hot-Spot: DNA-Barcoding Reveals Patterns of Evolution among Antarctic Demosponges (Class Demospongiae, Phylum Porifera)

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0127573 ◽  
Author(s):  
Sergio Vargas ◽  
Michelle Kelly ◽  
Kareen Schnabel ◽  
Sadie Mills ◽  
David Bowden ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Hot Spot

scholarly journals DNA barcoding and traditional taxonomy: an integrated approach for biodiversity conservation

Genome ◽  
2017 ◽  
Vol 60 (7) ◽  
pp. 618-628 ◽  
Author(s):  
Bhavisha P. Sheth ◽  
Vrinda S. Thaker
Keyword(s):  
Dna Barcoding ◽  
Biodiversity Conservation ◽  
Biological Diversity ◽  
Integrated Approach ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Conservation Practice ◽  
Dna Taxonomy ◽  
Research Areas ◽  
Traditional Taxonomy

Biological diversity is depleting at an alarming rate. Additionally, a vast amount of biodiversity still remains undiscovered. Taxonomy has been serving the purpose of describing, naming, and classifying species for more than 250 years. DNA taxonomy and barcoding have accelerated the rate of this process, thereby providing a tool for conservation practice. DNA barcoding and traditional taxonomy have their own inherent merits and demerits. The synergistic use of both methods, in the form of integrative taxonomy, has the potential to contribute to biodiversity conservation in a pragmatic timeframe and overcome their individual drawbacks. In this review, we discuss the basics of both these methods of biological identification (traditional taxonomy and DNA barcoding), the technical advances in integrative taxonomy, and future trends. We also present a comprehensive compilation of published examples of integrative taxonomy that refer to nine topics within biodiversity conservation. Morphological and molecular species limits were observed to be congruent in ∼41% of the 58 source studies. The majority of the studies highlighted the description of cryptic diversity through the use of molecular data, whereas research areas like endemism, biological invasion, and threatened species were less discussed in the literature.

Sign in / Sign up

Export Citation Format

Share Document