Cyclic AMP-dependent secretion of Ca 19-9 by LS174T human colon carcinoma cells

2002 ◽  
Author(s):  
Vincenzo Macchia ◽  
Maria Gargiulo ◽  
Daniela Terracciano ◽  
Angelina Di Carlo ◽  
Angela Mariano
Keyword(s):  
Cyclic Amp ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

scholarly journals Cyclic AMP-independent secretion of mucin by SW1116 human colon carcinoma cells. Differential control by Ca2+ ionophore A23187 and arachidonic acid

1992 ◽  
Vol 283 (2) ◽  
pp. 421-426 ◽  
Author(s):  
S Yedgar ◽  
O Eidelman ◽  
E Malden ◽  
D Roberts ◽  
R Etcheberrigaray ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Arachidonic Acid ◽  
Cyclic Amp ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Ionophore A23187 ◽  
Mucin Secretion ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

The regulation of mucin secretion by SW1116 human colon carcinoma cells has been studied using monoclonal antibody 19-9, which has previously been used to detect mucin in the serum of cancer and cystic fibrosis patients. We found that SW1116 cells constitutively secrete considerable amounts of mucin as the predominant glycoprotein. The secretion of mucin by these cells is independent of cyclic AMP levels, but can be further stimulated by the Ca2+ ionophore A23187. However, arachidonic acid and its metabolites inhibit mucin secretion. Electron microscope studies reveal that the mucin is located near the plasma membrane as well as in vesicular and lysosome-like structures. However, the secretion pathway of mucin is different than that of the lysosomal contents, since arachidonic acid, while inhibiting mucin secretion, actually activates the secretion of the lysosomal enzyme beta-glucuronidase. We suggest that the mechanism of mucin secretion by SW1116 cells occurs by a pathway different from common exocytosis, and possibly by more than one pathway. The response of mucin secretion by SW1116 cells to common secretagogues resembles that of epithelial cells obtained from cystic fibrosis patients. Thus SW1116 cells are an especially interesting system for studying processes related to pathological states associated with excessive constitutive secretion of mucin.

The influence of extracts from Rubus caesius leaves on human colon carcinoma cells cultured in vitro

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
R Paduch ◽  
M Tomczyk ◽  
A Wiater ◽  
A Dudek ◽  
M Pleszczynska ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma ◽  
Cells Cultured

scholarly journals Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.

1990 ◽  
Vol 265 (32) ◽  
pp. 19980-19989
Author(s):  
R V Iozzo ◽  
I Kovalszky ◽  
N Hacobian ◽  
P K Schick ◽  
J S Ellingson ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Heparan Sulfate Proteoglycan ◽  
Carcinoma Cells ◽  
Sulfate Proteoglycan ◽  
Fatty Acylation ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

scholarly journals Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

2009 ◽  
Vol 15 (9) ◽  
pp. 1079 ◽  
Author(s):  
Piet Habbel ◽  
Karsten H Weylandt ◽  
Katja Lichopoj ◽  
Johannes Nowak ◽  
Martin Purschke ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Docosahexaenoic Acid ◽  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma
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