Prediction of cell kill kinetics of anticancer agents using the collagen gel droplet embedded-culture drug sensitivity test

2002 ◽  
Author(s):  
Takeshi Mori ◽  
Masafumi Ohnishi ◽  
Megumi Komiyama ◽  
Arisa Tsutsui ◽  
Hiromitsu Yabushita ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test ◽  
Cell Kill ◽  
Kinetics Of

scholarly journals Stratified phase II trial to establish the usefulness of the collagen gel droplet embedded culture-drug sensitivity test (CD-DST) for advanced gastric cancer

2013 ◽  
Vol 17 (4) ◽  
pp. 630-637 ◽  
Author(s):  
Hiroyuki Naitoh ◽  
Hiroshi Yamamoto ◽  
Satoshi Murata ◽  
Hisayuki Kobayashi ◽  
Katsunori Inoue ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Phase Ii ◽  
Drug Sensitivity ◽  
Phase Ii Trial ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test

The Collagen Gel Droplet–embedded Culture Drug Sensitivity Test in Relapsed Hepatoblastoma

2017 ◽  
Vol 39 (5) ◽  
pp. 395-401 ◽  
Author(s):  
Hiroaki Goto ◽  
Norihiko Kitagawa ◽  
Hironobu Sekiguchi ◽  
Yohei Miyagi ◽  
Dai Keino ◽  
...  
Keyword(s):  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test

Personalized first-line chemotherapy for advanced colorectal cancer using the collagen gel droplet-embedded culture drug sensitivity test.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e14523-e14523
Author(s):  
Takumi Ochiai ◽  
Kazuhiko Nishimura ◽  
Tomoo Watanabe ◽  
Masayuki Kitajima ◽  
Akinori Nakatani ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Sensitivity ◽  
Advanced Colorectal Cancer ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
First Line ◽  
Drug Sensitivity Test ◽  
Line Chemotherapy

In vitro-in vivo Correlation in Anticancer Drug Sensitivity Test Using AUC-Based Concentrations and Collagen Gel Droplet-Embedded Culture

Oncology ◽  
1996 ◽  
Vol 53 (3) ◽  
pp. 250-257 ◽  
Author(s):  
Makoto Inaba ◽  
Tazuko Tashiro ◽  
Shigeo Sato ◽  
Yasuyuki Ohnishi ◽  
Keizo Tanisaka ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test

A study for establishment of individualized chemotherapy for colorectal cancer using the area under the concentration curve (AUC) based on the collagen gel droplet embedded culture-drug sensitivity test (CD-DST)

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 13560-13560
Author(s):  
T. Ochiai ◽  
K. Nishimura ◽  
H. Noguchi ◽  
M. Kitajima ◽  
A. Tsukada ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Concentration Curve ◽  
Drug Sensitivity Test ◽  
Logarithmic Curve ◽  
The Individual ◽  
Individualized Chemotherapy

13560 Background: The drug sensitivity of tumor cells is one of the key issues to explore for individualized chemotherapy for cancer patients. We reported the 5-fluorouracil (5-FU) sensitivity of cancer cells from colorectal cancer (CRC) patients using the collagen gel droplet embedded culture-drug sensitivity test (CD-DST) under multiple drug concentrations and contact duration. We also reported that growth inhibition rate (IR) and area under the concentration curve (AUC) approximated to logarithmic curve in ASCO 2003 (#1283). The aim of this study was to evaluate the individual 50% inhibitory AUC (AUCIR50) and research the usefulness of individual AUCIR50 for establishment of individualized chemotherapy. Methods: Surgical specimen was obtained from resectable 53 CRC patients without any preoperative chemotherapy during 2002 to 2005. CD-DST was performed under nine different conditions: concentration of 5-FU tested: 0.2, 1 and 10μg/ml; duration of exposure: 3, 24 and 120 hours. After converting drug concentration and contact time to AUC and plotting against growth IR, individual correlation between AUC and growth IR was evaluated. The histogram of individual AUCIR50 was also evaluated. Results: The curve between AUC and growth IR approximated to logarithmic curve in all of the patients (R2=0.69–0.96). We could calculate the individual AUCIR50 in all of the patients (AUCIR50= 23.7–98796298.1μg*hr/ml). The histogram of the individual AUCIR50 (AUCIR50<2500μg*hr/ml) indicated CURT(X) pattern (SK=0.0076). Conclusions: We could obtain a position for each patient on the histogram of AUCIR50. We could also indicate the potential for 5-FU sensitivity to each patient. Some patients demonstrating low 5-FU sensitivity could not be recommended for 5-FU based chemotherapy, and non-5-FU chemotherapy should be explored for them. This study demonstrated that the individual AUCIR50 is useful to distinguish the individualized chemotherapy of colorectal cancer patients. Utilization of CD-DST will facilitate establishment of individualized chemotherapy for colorectal cancer. No significant financial relationships to disclose.

scholarly journals Individualized chemotherapy for colorectal cancer based on the collagen gel droplet-embedded drug sensitivity test

2012 ◽  
Vol 4 (4) ◽  
pp. 621-624 ◽  
Author(s):  
TAKUMI OCHIAI ◽  
KAZUHIKO NISHIMURA ◽  
TOMOO WATANABE ◽  
MASAYUKI KITAJIMA ◽  
AKINORI NAKATANI ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test ◽  
Individualized Chemotherapy
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