Wild-type p53 gene transfection in human cultured sarcomas: Effect of CDDP

2001 ◽  
Author(s):  
Koji Endo ◽  
Itaru Kuratate ◽  
Mari Watanabe ◽  
Haruhiko Yoshida ◽  
Ryota Teshima ◽  
...  
Keyword(s):  
Gene Transfection ◽  
P53 Gene ◽  
Wild Type ◽  
Wild Type P53

scholarly journals Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells

2012 ◽  
Vol 3 (6) ◽  
pp. 999-1004 ◽  
Author(s):  
WEN-JUAN CHEN ◽  
ZHENG-AI XIONG ◽  
YAN TANG ◽  
PEI-TING DONG ◽  
PAN LI ◽  
...  
Keyword(s):  
Hela Cells ◽  
Gene Transfection ◽  
P53 Gene ◽  
Wild Type ◽  
Wild Type P53

Effect of p53 gene transfection on proliferation, apoptosis, and radiosensitivity of cervical cancer cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e22024-e22024
Author(s):  
Xiaofan Li ◽  
Shao Wen ◽  
Shanwen Zhang
Keyword(s):  
Cell Proliferation ◽  
Gene Transfection ◽  
P53 Gene ◽  
Wild Type ◽  
Transfected Cells ◽  
Siha Cells ◽  
Protein Expressions ◽  
Cervical Cancer Cells ◽  
Wild Type P53 ◽  
Human Wild Type

e22024 Background: To investigate the effects of p53 gene transfection on proliferation, apoptosis and radio-sensitivity of cervical cancer cells. Methods: Two cervical cell lines: Siha with wild-type p53 gene and C33a with mutant p53 gene, were transfected with human wild-type p53 gene. Un-transfected cells and transfected cells were exposed to 6 Gy of radiation. Cell proliferation, apoptosis, and protein expressions of p53, MDM2, p21, and bax in the un-treated, p53- transfected, and irradiated cells were analyzed. Results: The protein expression of p53, MDM2, p21, and bax in the untreated Siha cells is very low; High level p53 and MDM2 proteins and low level of p21 and bax proteins were found in the untreated C33a cells. When transfected with recombinant adenoviral human wild-type p53 gene (rAd-p53) the protein levels of p53, MDM2, p21, and bax in both Siha and C33a increased. Highest levels of these proteins were detected at 48 hours after transfection. The increased protein expressions were accompanied with inhibition of cell proliferation. At 72 hours after transfection, 12.3% and 5.5% of Siha and C33a cells became apoptotic, respectively. When exposed to 6 Gy of radiation after 48 hours of the transfection, 46.2% and 38.3% apoptotic Siha and C33a cells were detected, respectively; but only 5.6% and 6.3% of apoptotic cells of their un-transfected counterparts detected at 24 hours after the radiation treatment. Conclusions: Wild-type p53 gene transfection can inhibit cell proliferation, promote apoptosis, and sensitize to radiation of both Siha and C33a cells. The effects on Siha cells are stronger.

scholarly journals Tumor Eradication by Wild-type p53-specific Cytotoxic T Lymphocytes

1997 ◽  
Vol 186 (5) ◽  
pp. 695-704 ◽  
Author(s):  
Michel P.M. Vierboom ◽  
Hans W. Nijman ◽  
Rienk Offringa ◽  
Ellen I.H. van der Voort ◽  
Thorbald van Hall ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Tumor Cells ◽  
Cytotoxic T Lymphocytes ◽  
Normal Tissue ◽  
P53 Protein ◽  
P53 Gene ◽  
Wild Type ◽  
Wild Type P53 ◽  
Tumor Outgrowth ◽  
Tumor Eradication

The tumor suppressor protein p53 is overexpressed in close to 50% of all human malignancies. The p53 protein is therefore an attractive target for immunotherapy. Cytotoxic T lymphocytes (CTLs) recognizing a murine wild-type p53 peptide, presented by the major histocompatibility complex class I molecule H-2Kb, were generated by immunizing p53 gene deficient (p53 −/−) C57BL/6 mice with syngeneic p53-overexpressing tumor cells. Adoptive transfer of these CTLs into tumor-bearing p53 +/+ nude mice caused complete and permanent tumor eradication. Importantly, this occurred in the absence of any demonstrable damage to normal tissue. When transferred into p53 +/+ immunocompetent C57BL/6 mice, the CTLs persisted for weeks in the absence of immunopathology and were capable of preventing tumor outgrowth. Wild-type p53-specific CTLs can apparently discriminate between p53-overexpressing tumor cells and normal tissue, indicating that widely expressed autologous molecules such as p53 can serve as a target for CTL-mediated immunotherapy of tumors.

scholarly journals Transferrin receptor targeting nanomedicine delivering wild-type p53 gene sensitizes pancreatic cancer to gemcitabine therapy

2013 ◽  
Vol 20 (4) ◽  
pp. 222-228 ◽  
Author(s):  
E R Camp ◽  
C Wang ◽  
E C Little ◽  
P M Watson ◽  
K F Pirollo ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Transferrin Receptor ◽  
P53 Gene ◽  
Wild Type ◽  
Receptor Targeting ◽  
Wild Type P53
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