Expression of the SART1 tumor-rejection antigen in hepatocellular carcinomas

2001 ◽  
Author(s):  
S. Yutani ◽  
S. Shichijo ◽  
Y. Inoue ◽  
N. Kawagoe ◽  
K. Okuda ◽  
...  
Keyword(s):  
Tumor Rejection ◽  
Hepatocellular Carcinomas ◽  
Tumor Rejection Antigen

scholarly journals Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa

2010 ◽  
Vol 12 (3) ◽  
pp. 344-355 ◽  
Author(s):  
Duvvuri Butchi Kameshwari ◽  
Satish Bhande ◽  
Curam Sreenivasacharlu Sundaram ◽  
Venkatesh Kota ◽  
Archana B. Siva ◽  
...  
Keyword(s):  
Tumor Rejection ◽  
Protein Precursor ◽  
Tumor Rejection Antigen ◽  
Epididymal Spermatozoa ◽  
Glucose Regulated Protein

scholarly journals The gene coding for a major tumor rejection antigen of tumor P815 is identical to the normal gene of syngeneic DBA/2 mice.

1991 ◽  
Vol 173 (6) ◽  
pp. 1373-1384 ◽  
Author(s):  
B Van den Eynde ◽  
B Lethé ◽  
A Van Pel ◽  
E De Plaen ◽  
T Boon
Keyword(s):  
Mast Cell ◽  
Tumor Rejection ◽  
Cosmid Library ◽  
Adult Mice ◽  
Gene Coding ◽  
Tumor Rejection Antigen ◽  
Normal Gene ◽  
Mast Cell Line ◽  
High Level

We showed previously that mouse mastocytoma P815 expresses several distinct antigens that are recognized by cytolytic T lymphocytes (CTL) of syngeneic DBA/2 mice. Antigens P815A and P815B are usually lost jointly and are targets for immune rejection responses in vivo. We used a cosmid library and a CTL stimulation assay to obtain transfectants expressing tumor rejection antigen P815A. From these transfectants we retrieved gene P1A which transferred the expression of both P815A and B. This gene is unrelated to three previously isolated genes coding for tum-antigens. It encodes a putative protein of 224 amino acids which contains two highly acidic domains showing homology with similar regions of nuclear proteins. The P1A gene expressed by tumor P815 is completely identical to the gene present in normal DBA/2 cells. Expression of the gene was tested by Northern blots. Cells from liver, spleen, and a number of mast cell lines were negative, but mast cell line L138.8A produced a high level of P1A message and was lysed by CTL directed against antigens P815A and B. We conclude that major tumor rejection antigens of P815 are encoded by a gene showing little or no expression in most normal cells of adult mice.

Identification and expression distribution of esophageal carcinoma autoantigens.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16520-e16520
Author(s):  
Fuchun Si
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Esophageal Carcinoma ◽  
Normal Tissue ◽  
Proteome Analysis ◽  
Tumor Rejection ◽  
Cancer Tissue ◽  
Carcinoma Tissue ◽  
Heat Shock Protein Gp96 ◽  
Tumor Rejection Antigen

e16520 Background: To identify the autoantigen protein molecules with autoserum in the tissues from the esophageal carcinoma (EC) patients, analyze autoantigen expression distribution in EC tissues, so as to provide basis for the molecular pathogenesis and clinical medication of EC. Methods: 69 cases of EC patients tissues and serum and 81 cases of healthy people serum were collected, serological proteome analysis (SERPA) was modified with sequential extraction of subcellular protein fractions to identify esophageal oncopathgensis stages autoantigen with autoserum in the tissues from the EC patients. Another 93 cases of EC patients tissue were collected, immunohistochemical and western blot method were used to detect expression distribution of EC autoantigens in esophageal carcinoma tissue, para-carcinoma tissue and normal tissue. Results: Autoantigens CK13, CK16, CaD, ACTG2, tumor rejection antigen (gp96) 1 variant, heat shock protein gp96 precursor were identified, among wihich, CK16, CaD, ACTG2, tumor rejection antigen (gp96)1 variant, heat shock protein gp96 precursor were firstly reported as EC autoantigens. Expression of autoantigens CK16, CaD, ACTG2 were increased in EC carcinoma tissue than para-carcinoma tissue and normal tissue, while CK13 were decreased. Positive expression level of CK16 in normal tissue, para-carcinoma tissue and cancer tissue of EC patients was 0.0076±0.0033, 0.0158±0.0065, 0.0356±0.0165 respectively, CaD was 0.0085±0.0048, 0.0107±0.0056, 0.0177±0.0103 respectively, ACTG2 was 0.0091±0.0039, 0.0136±0.0043, 0.0214±0.0110 respectively, and CK13 was 0.2053±0.0311, 0.1633±0.0280, 0.0412±0.0239 respectively. Conclusions: 6 EC autoantigens were identified, and 5 were first reported. Autoantigens CK13, CK16, CaD and ACTG2 were expressed in EC patients carcinoma tissue, which can be the potential biomarkers of esophageal carcinoma. This study provides new basis for the EC molecular mechanism and development of molecular drugs.

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